P39483 (DHG2_BACME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose 1-dehydrogenase 2
EC=1.1.1.47

Alternative name(s):
GLCDH-II

Gene names

Name:

gdhII

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer.
Miscellaneous	Prefers NADP to NAD.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	glucose 1-dehydrogenase [NAD(P)] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Glucose 1-dehydrogenase 2		PRO_0000054610
Regions
	Nucleotide binding	11 – 35	25	NADP By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39483 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: CEF9AA1425D2999C	FASTA	261	28,251
	10 20 30 40 50 60 MYTDLKDKVV VVTGGSKGLG RAMAVRFGQE QSKVVVNYRS NEEEALEVKK EIEEAGGQAI 70 80 90 100 110 120 IVRGDVTKEE DVVNLVETAV KEFGSLDVMI NNAGVENPVP SHELSLENWN QVIDTNLTGA 130 140 150 160 170 180 FLGSREAIKY FVENDIKGNV INMSSVHEMI PWPLFVHYAA SKGGMKLMTE TLALEYAPKG 190 200 210 220 230 240 IRVNNIGPGA IDTPINAEKF ADPEQRADVE SMIPMGYIGK PEEIASVAAF LASSQASYVT 250 260 GITLFADGGM TKYPSFQAGR G

References

[1]	"Structure of isozyme genes of glucose dehydrogenase from Bacillus megaterium IAM1030." Mitamura T., Ebora R.V., Nakai T., Makino Y., Negoro S., Urabe I., Okada H. J. Ferment. Bioeng. 70:363-369(1990) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1030.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90044 Genomic DNA. Translation: BAA14100.1.
PIR	I39853.
3D structure databases
ProteinModelPortal	P39483.
SMR	P39483. Positions 1-261.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHG2_BACME

Accession

Primary (citable) accession number: P39483

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program