Reviewed, UniProtKB/Swiss-Prot P39482 (DHG1_BACME)

Names and origin

Protein names

Recommended name:
    Glucose 1-dehydrogenase 1
    EC=1.1.1.47
Alternative name(s):
    GLCDH-I

Gene names

Name:

gdhI

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play some role in spore germination.
Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer.
Developmental stage	Expressed during sporulation.
Miscellaneous	Prefers NADP to NAD.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Germination Sporulation
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Glucose 1-dehydrogenase 1		PRO_0000054609
Regions
	Nucleotide binding	11 – 35	25	NADP By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39482-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B92B72B89A160335	FASTA	261	28,117
	10 20 30 40 50 60 MYKDLEGKVV VITGSSTGLG KAMAIRFATE KAKVVVNYRS KEEEANSVLE EIKKVGGEAI 70 80 90 100 110 120 AVKGDVTVES DVINLVQSSI KEFGKLDVMI NNAGMENPVS SHEMSLSDWN KVIDTNLTGA 130 140 150 160 170 180 FLGSREAIKY FVENDIKGTV INMSSVHEKI PWPLFVHYAA SKGGMKLMTE TLALEYAPKG 190 200 210 220 230 240 IRVNNIGPGA INTPINAEKF ADPEQRADVE SMIPMGYIGE PEEIAAVAAW LASSEASYVT 250 260 GITLFADGGM TQYPSFQAGR G

References

[1]	"Structure of isozyme genes of glucose dehydrogenase from Bacillus megaterium IAM1030." Mitamura T., Ebora R.V., Nakai T., Makino Y., Negoro S., Urabe I., Okada H. J. Ferment. Bioeng. 70:363-369(1990) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1030.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90043 Genomic DNA. Translation: BAA14099.1.
PIR	JS0385. I39852.
3D structure databases
SMR	P39482. Positions 1-261.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.47. 325.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHG1_BACME

Accession

Primary (citable) accession number: P39482

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)