Reviewed, UniProtKB/Swiss-Prot P39475 (DHE4_SULSH)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:

gdhA

Organism

Sulfolobus shibatae

Taxonomic identifier

2286 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	390 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 390	›390	NADP-specific glutamate dehydrogenase		PRO_0000182781
Sites
	Active site	81	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P39475-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BCB9ACFE6AC9CEB2	FASTA	390	42,091
	10 20 30 40 50 60 LDTLEALSQP ERVIQVKIQI RGSDGKLKTF MGWRSQHNSA LGPYKGGVRY SPNVTQDEVI 70 80 90 100 110 120 ALSMIMTWKN SLLLLPYGGG KGGIRVDPKK LTLKELEDLS RKYVQLIHNY LGSDVDIPAP 130 140 150 160 170 180 DINTNPQTMA WFLDEYIKIT GEVDFAVFTG KPSELGGIGV RLYSTGLGVA TIAREAANKF 190 200 210 220 230 240 IGGIEGSRVI IQGFGNVGSF TAKFLNEMGA KIIGVSDIGG GVISDDGIDV NKALEVVQST 250 260 270 280 290 300 GSVVNYPEGK KVTNEELLTS DCDILIPAAV ENVINKFNAP KVKAKLIVEG ANGPLAADAD 310 320 330 340 350 360 EIIKQRGIVV IPDILANAGG VVGSYVEWAN NKSGGIISDE EAKKLIIDRM TNAFNALYEF 370 380 390 HKRKFADQDL RTVAMALRVD RVVGMKARAI

References

[1]

"PCR-mediated cloning and sequencing of the gene encoding glutamate dehydrogenase from the archaeon Sulfolobus shibatae: identification of putative amino-acid signatures for extremophilic adaptation." Benachenhou-Lahfa N., Labedan B., Forterre P. Gene 140:17-24(1994) [PubMed: 8125334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73990 Genomic DNA. Translation: CAA52168.1.
PIR	S37407.
3D structure databases
HSSP	HSSP built from PDB template 2TMG based on UniProtKB P96110.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 2332.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_SULSH

Accession

Primary (citable) accession number: P39475

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)