Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase subunit E

Gene

rpoE

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BRENDAi2.7.7.6. 6160.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit E (EC:2.7.7.6)
Gene namesi
Name:rpoE
Ordered Locus Names:Saci_0834
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000740381 – 183DNA-directed RNA polymerase subunit EAdd BLAST183

Interactioni

Subunit structurei

The S.acidocaldarius RNAP is composed of 13 subunits.

Protein-protein interaction databases

STRINGi330779.Saci_0834.

Structurei

3D structure databases

ProteinModelPortaliP39466.
SMRiP39466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 164S1 motifPROSITE-ProRule annotationAdd BLAST83

Phylogenomic databases

eggNOGiarCOG00675. Archaea.
COG1095. LUCA.
HOGENOMiHOG000229315.
KOiK03049.
OMAiSYFGPRM.
OrthoDBiPOG093Z0C8D.

Family and domain databases

Gene3Di3.30.1490.120. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR005576. RNA_pol_Rpb7_N.
IPR004519. RNAP_E/RPC8.
IPR022967. S1_dom.
IPR003029. S1_domain.
PfamiView protein in Pfam
PF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
SMARTiView protein in SMART
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
TIGRFAMsiTIGR00448. rpoE. 1 hit.
PROSITEiView protein in PROSITE
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

P39466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKLVRAKGI VRIPPEYFGQ SVDEIAIKIL RQEYQEKLIK DIGVVLGIVN
60 70 80 90 100
AKASEEGFII FGDGATYHEV EFDMLVYTPI IHEVIEGEVS QVDNYGVYVN
110 120 130 140 150
MGPVDGLVHI SQITDDNLKF DSNRGILIGE KSKKSIQKGD RVRAMIISAS
160 170 180
MSSGRLPRIA LTMKQPYLGK IEWINQEIAK ASK
Length:183
Mass (Da):20,471
Last modified:August 30, 2005 - v2
Checksum:iDA621E0357A2E2B9
GO

Sequence cautioni

The sequence CAA53164 differs from that shown. Reason: Frameshift at position 170.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137Q → T in CAA53164 (PubMed:8127719).Curated1
Sequence conflicti182S → R AA sequence (PubMed:8127719).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75411 Genomic DNA. Translation: CAA53164.1. Frameshift.
CP000077 Genomic DNA. Translation: AAY80201.1.
PIRiS42389. S38658.
RefSeqiWP_011277703.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY80201; AAY80201; Saci_0834.
GeneIDi3472705.
KEGGisai:Saci_0834.
PATRICifig|330779.12.peg.798.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRPOE_SULAC
AccessioniPrimary (citable) accession number: P39466
Secondary accession number(s): Q4JAH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 30, 2005
Last modified: June 7, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome