P39465 (GPT_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
EC=2.7.8.15

Alternative name(s):
GlcNAc-1-P transferase
Short name=G1PT
Short name=GPT
N-acetylglucosamine-1-phosphate transferase

Gene names

Name:	gnpTA
Ordered Locus Names:	Saci_0093

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus acidocaldarius

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.
Enzyme regulation	Inhibited by tunicamycin By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 4 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Inferred from electronic annotation. Source: EC phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase		PRO_0000108766
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	107 – 127	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Transmembrane	228 – 248	21	Helical; Potential
	Transmembrane	301 – 321	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P39465 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 01798FA2BD3297D9	FASTA	328	35,472
	10 20 30 40 50 60 MLVSLLGILL SVIVGFVVTL ISTKWVIGLC KKRGFTGKDI NKLTKDDVPV LGGIGIVAGF 70 80 90 100 110 120 VAGSFTFLLT SYNLSPGIEN VVVSILLSSL IIGFLGLLDD IFNISQATRA FLPIFASIPL 130 140 150 160 170 180 ILYSVGHTII SIPFLGKVNF GILFYIIILP ATLTITANAF NMLEGLNGLG AGMGLIMALA 190 200 210 220 230 240 LAYIGLKSGG TSFYAGIVSI ILASVLFGFL IFNFYPAKTF PGNIGTYFIG SVIGSIGISG 250 260 270 280 290 300 YMYTALFFLY LPYVIEFVLK AKTRFKGVSF GKIDDQGYLH WDSKPNSLTH IVMRIGKFKE 310 320 YHIVLIIWGI EILFAILAVV FQTVTITI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Archaebacterial ether-linked lipid biosynthetic gene. Expression cloning, sequencing, and characterization of geranylgeranyl-diphosphate synthase." Ohnuma S., Suzuki M., Nishino T. J. Biol. Chem. 269:14792-14797(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28748 Genomic DNA. Translation: BAA05941.1. CP000077 Genomic DNA. Translation: AAY79520.1.
PIR	B54058.
RefSeq	YP_254813.1. NC_007181.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	330779.Saci_0093.
Protocols and materials databases
DNASU	3473869.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY79520; AAY79520; Saci_0093.
GeneID	3473869.
KEGG	sai:Saci_0093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0472.
HOGENOM	HOG000227679.
KO	K01001.
OMA	IVLLPNI.
ProtClustDB	CLSK802540.
Enzyme and pathway databases
BioCyc	SACI330779:GH9J-178-MONOMER.
Family and domain databases
InterPro	IPR000715. Glycosyl_transferase_4. [Graphical view]
PANTHER	PTHR22926. PTHR22926. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPT_SULAC

Accession

Primary (citable) accession number: P39465
Secondary accession number(s): Q4JCF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program