Reviewed, UniProtKB/Swiss-Prot P39461 (FUMC_SULSO)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	SSO1077

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as an auxiliary aerobic enzyme in the citric acid cycle. HAMAP MF_00743
Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer. HAMAP MF_00743
Subcellular location	Cytoplasm. HAMAP MF_00743
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 8.0. HAMAP MF_00743 Temperature dependence: Optimum temperature is 85 degrees Celsius. Active from 40 to 90 degrees Celsius.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161334
Regions
	Region	101 – 104	4	B site By similarity
	Region	111 – 113	3	Substrate binding By similarity
Sites
	Binding site	78	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39461-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8D2A9CF37817EA6C	FASTA	438	47,911
	10 20 30 40 50 60 MKYTDTAPKL FMNTGTKFPR RIIWSMGVLK KSCAKVNADL GLLDKKIADS IIKASDDLID 70 80 90 100 110 120 GKLDDKIVLD VFQTGSGTGL NMNVNEVIAE VASSYSNLKV HPNDHVNFGQ SSNDTVPTAI 130 140 150 160 170 180 RIAAVAEVTN RLLPALQQII SSLNKKAEEY KDVIKAGRTH LRDALPVTLG QELSAYADAF 190 200 210 220 230 240 QHEHEQVMNI LEYVKELPIG GTATGTGLNS HPEFQERVIN EINRITGLGF KPANRFRAMR 250 260 270 280 290 300 LLTDLLLLSG ALRNIAVDLY RLGQDIRLMF SGPLTGLNEI DLPTQEEIAG SSIMPGKTNP 310 320 330 340 350 360 VTVEATLLIS AQVVGLDHAN QFASMLGEFE LSMGIPLVGY NIVTQVNFIS EALEKMSRLV 370 380 390 400 410 420 IDGMVANVEK MKRYAESSPS LITIVSPVIG YDKATEIGKK LNKGMSIREA LRELGYSDNE 430 INKILDLSKL VKPGFTAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, nucleotide sequence and expression of a Sulfolobus solfataricus gene encoding a class II fumarase." Colombo S., Grisa M., Tortora P., Vanoni M. FEBS Lett. 337:93-98(1994) [PubMed: 8276121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14. Strain: DSM 5833 / MT-4.
[2]	Erratum Colombo S., Grisa M., Tortora P., Vanoni M. FEBS Lett. 340:151-153(1994) [PubMed: 8119400] [Abstract]
[3]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[4]	"Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus." Puchegger S., Redl B., Stoeffler G. J. Gen. Microbiol. 136:1537-1541(1990) [PubMed: 2124611] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75402 Genomic DNA. Translation: CAA53156.1. AE006641 Genomic DNA. Translation: AAK41339.1. Different initiation.
PIR	D90260. S40448.
3D structure databases
HSSP	HSSP built from PDB template 1YFM based on UniProtKB P08417.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus SSO1077 in contig AE006641_GR.
KEGG	sso:SSO1077.
NMPDR	fig|273057.1.peg.972.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P39461.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1077-MON.
BRENDA	4.2.1.2. 2070.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_SULSO

Accession

Primary (citable) accession number: P39461

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)