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Protein

Nitrate reductase

Gene

narB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi26 – 261Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi30 – 301Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi70 – 701Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. molybdenum ion binding Source: InterPro
  4. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. denitrification pathway Source: UniProtKB-UniPathway
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13441.
SYNEL:SYNPCC7942_1235-MONOMER.
BRENDAi1.7.7.2. 6187.
UniPathwayiUPA00652; UER00706.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:narB
Ordered Locus Names:Synpcc7942_1235
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Nitrate reductasePRO_0000063237Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PETFP002212EBI-932578,EBI-864933From a different organism.

Protein-protein interaction databases

IntActiP39458. 2 interactions.
STRINGi1140.Synpcc7942_1235.

Structurei

3D structure databases

ProteinModelPortaliP39458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 84694Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
KOiK00367.
OrthoDBiEOG6CVV7G.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39458-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG
60 70 80 90 100
TPIWQIRGDR QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE
110 120 130 140 150
ISWDEALDRL CDRIQQTQAD YGKDGICFYG SGQFQTEDYY IAQKLVKGCL
160 170 180 190 200
GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP ACYEDLDLAD CLLIVGSNTA
210 220 230 240 250
ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA LKPGSDVALL
260 270 280 290 300
NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE
310 320 330 340 350
LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC
360 370 380 390 400
GPFSLTGQPN AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI
410 420 430 440 450
SPQPGRTAWQ MIEGLEQGAV GFLWVAATNP AVSLPDVKRA QAALKRSPFT
460 470 480 490 500
VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT MTNSERRVTL CQAFRQPPGE
510 520 530 540 550
ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC DLSGLSHELL
560 570 580 590 600
AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP
610 620 630 640 650
DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI
660 670 680 690 700
SEGQAVVIRS RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT
710 720
HPAACPISGE PELKACAVQI EAASSTFTI
Length:729
Mass (Da):79,854
Last modified:April 18, 2006 - v2
Checksum:i455798C8631755A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti615 – 6162GH → PD in CAA52675. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74597 Genomic DNA. Translation: CAA52675.1.
CP000100 Genomic DNA. Translation: ABB57265.1.
X61625 Genomic DNA. Translation: CAA43813.1.
PIRiS36605.
RefSeqiYP_400252.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB57265; ABB57265; Synpcc7942_1235.
GeneIDi3773523.
KEGGisyf:Synpcc7942_1235.
PATRICi23787779. VBISynElo51371_1417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74597 Genomic DNA. Translation: CAA52675.1.
CP000100 Genomic DNA. Translation: ABB57265.1.
X61625 Genomic DNA. Translation: CAA43813.1.
PIRiS36605.
RefSeqiYP_400252.1. NC_007604.1.

3D structure databases

ProteinModelPortaliP39458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP39458. 2 interactions.
STRINGi1140.Synpcc7942_1235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB57265; ABB57265; Synpcc7942_1235.
GeneIDi3773523.
KEGGisyf:Synpcc7942_1235.
PATRICi23787779. VBISynElo51371_1417.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
KOiK00367.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

UniPathwayiUPA00652; UER00706.
BioCyciMetaCyc:MONOMER-13441.
SYNEL:SYNPCC7942_1235-MONOMER.
BRENDAi1.7.7.2. 6187.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Andriesse A.J., Bakker H.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.
  3. "Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942."
    Omata T., Andriesse X., Hirano A.
    Mol. Gen. Genet. 236:193-202(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

Entry informationi

Entry nameiNARB_SYNE7
AccessioniPrimary (citable) accession number: P39458
Secondary accession number(s): Q31NV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 18, 2006
Last modified: January 7, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.