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P39458 (NARB_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase

EC=1.7.99.4
Gene names
Name:narB
Ordered Locus Names:Synpcc7942_1235
OrganismSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]
Taxonomic identifier1140 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 1 4Fe-4S cluster Potential.

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 1/4.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PETFP002212EBI-932578,EBI-864933From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Nitrate reductase
PRO_0000063237

Regions

Domain16 – 84694Fe-4S Mo/W bis-MGD-type

Sites

Metal binding231Iron-sulfur (4Fe-4S) By similarity
Metal binding261Iron-sulfur (4Fe-4S) By similarity
Metal binding301Iron-sulfur (4Fe-4S) By similarity
Metal binding701Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict615 – 6162GH → PD in CAA52675. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39458 [UniParc].

Last modified April 18, 2006. Version 2.
Checksum: 455798C8631755A6

FASTA72979,854
        10         20         30         40         50         60 
MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG TPIWQIRGDR 

        70         80         90        100        110        120 
QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE ISWDEALDRL CDRIQQTQAD 

       130        140        150        160        170        180 
YGKDGICFYG SGQFQTEDYY IAQKLVKGCL GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP 

       190        200        210        220        230        240 
ACYEDLDLAD CLLIVGSNTA ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA 

       250        260        270        280        290        300 
LKPGSDVALL NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE 

       310        320        330        340        350        360 
LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC GPFSLTGQPN 

       370        380        390        400        410        420 
AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI SPQPGRTAWQ MIEGLEQGAV 

       430        440        450        460        470        480 
GFLWVAATNP AVSLPDVKRA QAALKRSPFT VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT 

       490        500        510        520        530        540 
MTNSERRVTL CQAFRQPPGE ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC 

       550        560        570        580        590        600 
DLSGLSHELL AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP 

       610        620        630        640        650        660 
DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI SEGQAVVIRS 

       670        680        690        700        710        720 
RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT HPAACPISGE PELKACAVQI 


EAASSTFTI 

« Hide

References

« Hide 'large scale' references
[1]Andriesse A.J., Bakker H.
Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7942.
[3]"Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942."
Omata T., Andriesse X., Hirano A.
Mol. Gen. Genet. 236:193-202(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74597 Genomic DNA. Translation: CAA52675.1.
CP000100 Genomic DNA. Translation: ABB57265.1.
X61625 Genomic DNA. Translation: CAA43813.1.
PIRS36605.
RefSeqYP_400252.1. NC_007604.1.

3D structure databases

ProteinModelPortalP39458.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP39458. 2 interactions.
STRING1140.Synpcc7942_1235.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB57265; ABB57265; Synpcc7942_1235.
GeneID3773523.
KEGGsyf:Synpcc7942_1235.
PATRIC23787779. VBISynElo51371_1417.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031440.
KOK00367.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13441.
SYNEL:SYNPCC7942_1235-MONOMER.
BRENDA1.7.7.2. 6187.
UniPathwayUPA00652; UER00706.

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARB_SYNE7
AccessionPrimary (citable) accession number: P39458
Secondary accession number(s): Q31NV4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways