P39458 (NARB_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase
EC=1.7.99.4

Gene names

Name:	narB
Ordered Locus Names:	Synpcc7942_1235

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus › Synechococcus elongatus

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin).
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 1/4.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	denitrification pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PETF	P00221	2	EBI-932578,EBI-864933	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Nitrate reductase		PRO_0000063237
Sites
	Metal binding	23	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	26	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	30	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	70	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Sequence conflict	615 – 616	2	GH → PD in CAA52675. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39458 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 455798C8631755A6	FASTA	729	79,854
	10 20 30 40 50 60 MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG TPIWQIRGDR 70 80 90 100 110 120 QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE ISWDEALDRL CDRIQQTQAD 130 140 150 160 170 180 YGKDGICFYG SGQFQTEDYY IAQKLVKGCL GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP 190 200 210 220 230 240 ACYEDLDLAD CLLIVGSNTA ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA 250 260 270 280 290 300 LKPGSDVALL NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE 310 320 330 340 350 360 LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC GPFSLTGQPN 370 380 390 400 410 420 AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI SPQPGRTAWQ MIEGLEQGAV 430 440 450 460 470 480 GFLWVAATNP AVSLPDVKRA QAALKRSPFT VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT 490 500 510 520 530 540 MTNSERRVTL CQAFRQPPGE ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC 550 560 570 580 590 600 DLSGLSHELL AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP 610 620 630 640 650 660 DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI SEGQAVVIRS 670 680 690 700 710 720 RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT HPAACPISGE PELKACAVQI EAASSTFTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Andriesse A.J., Bakker H. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7942.
[3]	"Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942." Omata T., Andriesse X., Hirano A. Mol. Gen. Genet. 236:193-202(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74597 Genomic DNA. Translation: CAA52675.1. CP000100 Genomic DNA. Translation: ABB57265.1. X61625 Genomic DNA. Translation: CAA43813.1.
PIR	S36605.
RefSeq	YP_400252.1. NC_007604.1.
3D structure databases
ProteinModelPortal	P39458.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39458. 2 interactions.
STRING	1140.Synpcc7942_1235.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABB57265; ABB57265; Synpcc7942_1235.
GeneID	3773523.
KEGG	syf:Synpcc7942_1235.
PATRIC	23787779. VBISynElo51371_1417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031440.
KO	K00367.
ProtClustDB	CLSK892855.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13441. SELO1140:GJWQ-1258-MONOMER.
BRENDA	1.7.7.2. 6187.
UniPathway	UPA00652; UER00706.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARB_SYNE7

Accession

Primary (citable) accession number: P39458
Secondary accession number(s): Q31NV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	April 18, 2006
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program