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P39458

- NARB_SYNE7

UniProt

P39458 - NARB_SYNE7

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Protein

Nitrate reductase

Gene
narB, Synpcc7942_1235
Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster Reviewed prediction.
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron-sulfur (4Fe-4S) By similarity
Metal bindingi26 – 261Iron-sulfur (4Fe-4S) By similarity
Metal bindingi30 – 301Iron-sulfur (4Fe-4S) By similarity
Metal bindingi70 – 701Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. molybdenum ion binding Source: InterPro
  4. nitrate reductase activity Source: UniProtKB-EC
  5. protein binding Source: IntAct

GO - Biological processi

  1. denitrification pathway Source: UniProtKB-UniPathway
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13441.
SYNEL:SYNPCC7942_1235-MONOMER.
BRENDAi1.7.7.2. 6187.
UniPathwayiUPA00652; UER00706.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:narB
Ordered Locus Names:Synpcc7942_1235
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Nitrate reductasePRO_0000063237Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PETFP002212EBI-932578,EBI-864933From a different organism.

Protein-protein interaction databases

IntActiP39458. 2 interactions.
STRINGi1140.Synpcc7942_1235.

Structurei

3D structure databases

ProteinModelPortaliP39458.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 84694Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031440.
KOiK00367.
OrthoDBiEOG6CVV7G.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39458-1 [UniParc]FASTAAdd to Basket

« Hide

MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG    50
TPIWQIRGDR QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE 100
ISWDEALDRL CDRIQQTQAD YGKDGICFYG SGQFQTEDYY IAQKLVKGCL 150
GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP ACYEDLDLAD CLLIVGSNTA 200
ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA LKPGSDVALL 250
NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE 300
LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC 350
GPFSLTGQPN AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI 400
SPQPGRTAWQ MIEGLEQGAV GFLWVAATNP AVSLPDVKRA QAALKRSPFT 450
VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT MTNSERRVTL CQAFRQPPGE 500
ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC DLSGLSHELL 550
AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP 600
DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI 650
SEGQAVVIRS RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT 700
HPAACPISGE PELKACAVQI EAASSTFTI 729
Length:729
Mass (Da):79,854
Last modified:April 18, 2006 - v2
Checksum:i455798C8631755A6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti615 – 6162GH → PD in CAA52675. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74597 Genomic DNA. Translation: CAA52675.1.
CP000100 Genomic DNA. Translation: ABB57265.1.
X61625 Genomic DNA. Translation: CAA43813.1.
PIRiS36605.
RefSeqiYP_400252.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB57265; ABB57265; Synpcc7942_1235.
GeneIDi3773523.
KEGGisyf:Synpcc7942_1235.
PATRICi23787779. VBISynElo51371_1417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74597 Genomic DNA. Translation: CAA52675.1 .
CP000100 Genomic DNA. Translation: ABB57265.1 .
X61625 Genomic DNA. Translation: CAA43813.1 .
PIRi S36605.
RefSeqi YP_400252.1. NC_007604.1.

3D structure databases

ProteinModelPortali P39458.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P39458. 2 interactions.
STRINGi 1140.Synpcc7942_1235.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB57265 ; ABB57265 ; Synpcc7942_1235 .
GeneIDi 3773523.
KEGGi syf:Synpcc7942_1235.
PATRICi 23787779. VBISynElo51371_1417.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031440.
KOi K00367.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00652 ; UER00706 .
BioCyci MetaCyc:MONOMER-13441.
SYNEL:SYNPCC7942_1235-MONOMER.
BRENDAi 1.7.7.2. 6187.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Andriesse A.J., Bakker H.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.
  3. "Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942."
    Omata T., Andriesse X., Hirano A.
    Mol. Gen. Genet. 236:193-202(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

Entry informationi

Entry nameiNARB_SYNE7
AccessioniPrimary (citable) accession number: P39458
Secondary accession number(s): Q31NV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 18, 2006
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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