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P39458

- NARB_SYNE7

UniProt

P39458 - NARB_SYNE7

Protein

Nitrate reductase

Gene

narB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.Curated
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi23 – 231Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi26 – 261Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi30 – 301Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi70 – 701Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. molybdenum ion binding Source: InterPro
    4. nitrate reductase activity Source: UniProtKB-EC
    5. protein binding Source: IntAct

    GO - Biological processi

    1. denitrification pathway Source: UniProtKB-UniPathway
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13441.
    SYNEL:SYNPCC7942_1235-MONOMER.
    BRENDAi1.7.7.2. 6187.
    UniPathwayiUPA00652; UER00706.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase (EC:1.7.99.4)
    Gene namesi
    Name:narB
    Ordered Locus Names:Synpcc7942_1235
    OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
    Taxonomic identifieri1140 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000002717: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Nitrate reductasePRO_0000063237Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PETFP002212EBI-932578,EBI-864933From a different organism.

    Protein-protein interaction databases

    IntActiP39458. 2 interactions.
    STRINGi1140.Synpcc7942_1235.

    Structurei

    3D structure databases

    ProteinModelPortaliP39458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 84694Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031440.
    KOiK00367.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39458-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG    50
    TPIWQIRGDR QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE 100
    ISWDEALDRL CDRIQQTQAD YGKDGICFYG SGQFQTEDYY IAQKLVKGCL 150
    GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP ACYEDLDLAD CLLIVGSNTA 200
    ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA LKPGSDVALL 250
    NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE 300
    LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC 350
    GPFSLTGQPN AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI 400
    SPQPGRTAWQ MIEGLEQGAV GFLWVAATNP AVSLPDVKRA QAALKRSPFT 450
    VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT MTNSERRVTL CQAFRQPPGE 500
    ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC DLSGLSHELL 550
    AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP 600
    DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI 650
    SEGQAVVIRS RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT 700
    HPAACPISGE PELKACAVQI EAASSTFTI 729
    Length:729
    Mass (Da):79,854
    Last modified:April 18, 2006 - v2
    Checksum:i455798C8631755A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti615 – 6162GH → PD in CAA52675. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74597 Genomic DNA. Translation: CAA52675.1.
    CP000100 Genomic DNA. Translation: ABB57265.1.
    X61625 Genomic DNA. Translation: CAA43813.1.
    PIRiS36605.
    RefSeqiYP_400252.1. NC_007604.1.

    Genome annotation databases

    EnsemblBacteriaiABB57265; ABB57265; Synpcc7942_1235.
    GeneIDi3773523.
    KEGGisyf:Synpcc7942_1235.
    PATRICi23787779. VBISynElo51371_1417.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74597 Genomic DNA. Translation: CAA52675.1 .
    CP000100 Genomic DNA. Translation: ABB57265.1 .
    X61625 Genomic DNA. Translation: CAA43813.1 .
    PIRi S36605.
    RefSeqi YP_400252.1. NC_007604.1.

    3D structure databases

    ProteinModelPortali P39458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P39458. 2 interactions.
    STRINGi 1140.Synpcc7942_1235.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB57265 ; ABB57265 ; Synpcc7942_1235 .
    GeneIDi 3773523.
    KEGGi syf:Synpcc7942_1235.
    PATRICi 23787779. VBISynElo51371_1417.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031440.
    KOi K00367.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    UniPathwayi UPA00652 ; UER00706 .
    BioCyci MetaCyc:MONOMER-13441.
    SYNEL:SYNPCC7942_1235-MONOMER.
    BRENDAi 1.7.7.2. 6187.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Andriesse A.J., Bakker H.
      Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7942.
    3. "Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942."
      Omata T., Andriesse X., Hirano A.
      Mol. Gen. Genet. 236:193-202(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

    Entry informationi

    Entry nameiNARB_SYNE7
    AccessioniPrimary (citable) accession number: P39458
    Secondary accession number(s): Q31NV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3