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Protein

Nitrate reductase

Gene

narB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrate + a quinol = nitrite + a quinone + H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: nitrate reduction (denitrification)

This protein is involved in step 1 of the subpathway that synthesizes dinitrogen from nitrate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Nitrate reductase (narB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway nitrate reduction (denitrification), which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dinitrogen from nitrate, the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi26Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi30Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi70Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13441
SYNEL:SYNPCC7942_1235-MONOMER
BRENDAi1.7.7.2 6187
UniPathwayiUPA00652; UER00706

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase (EC:1.7.5.1)
Gene namesi
Name:narB
Ordered Locus Names:Synpcc7942_1235
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000632371 – 729Nitrate reductaseAdd BLAST729

Proteomic databases

PRIDEiP39458

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PETFP002212EBI-932578,EBI-864933From Spinacia oleracea.

Protein-protein interaction databases

IntActiP39458, 2 interactors
STRINGi1140.Synpcc7942_1235

Structurei

3D structure databases

ProteinModelPortaliP39458
SMRiP39458
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 844Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031440
KOiK00367
OrthoDBiPOG091H04T5

Family and domain databases

InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR006655 Mopterin_OxRdtase_prok_CS
IPR027467 MopterinOxRdtase_cofactor_BS
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit

Sequencei

Sequence statusi: Complete.

P39458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDLSKFLPV ITPLMIDTAK TLCPYCGVGC GLEAVPPAQP GRATVRDREG
60 70 80 90 100
TPIWQIRGDR QHPSSQGMVC VKGATVAESV SKSRLKYPMF RASLDDPFTE
110 120 130 140 150
ISWDEALDRL CDRIQQTQAD YGKDGICFYG SGQFQTEDYY IAQKLVKGCL
160 170 180 190 200
GTNNFDTNSR LCMSSAVSAY SLCLGSDGPP ACYEDLDLAD CLLIVGSNTA
210 220 230 240 250
ECHPILFNRY RKRHKQGGTN LIVVDPRCTP TAEVADLHLA LKPGSDVALL
260 270 280 290 300
NGLGWLLYQM GYVKKDFIAN QTEGFEDWLA IIEDYPPQRT AELTGLAVAE
310 320 330 340 350
LVRAADLIAS AQRWLSLWSM GVNQSIQGTA KATSLINLHL LTRQIGLPGC
360 370 380 390 400
GPFSLTGQPN AMGGRETGGL AHLLPGYRKV IDPQHRADVE TIWGLPMGSI
410 420 430 440 450
SPQPGRTAWQ MIEGLEQGAV GFLWVAATNP AVSLPDVKRA QAALKRSPFT
460 470 480 490 500
VLQDAYHPTE TTTYAHLLLP AAQWSEKTGT MTNSERRVTL CQAFRQPPGE
510 520 530 540 550
ARADWQIFAE VGRRLGFAFD YTDAAAVFAE YVQVTAGRLC DLSGLSHELL
560 570 580 590 600
AQAGPQQWPF PAGAEPTTES KRLYTKHHFA YADGRARFQP FHHLGVAEPP
610 620 630 640 650
DDRYPLVLTV GRLYGHWHTQ TRTGRIDKIN KLHPSAFVEI HPRDADRYNI
660 670 680 690 700
SEGQAVVIRS RRGEGCFPAK VTTAISPGVL FVPMHWGALW GDRTEANALT
710 720
HPAACPISGE PELKACAVQI EAASSTFTI
Length:729
Mass (Da):79,854
Last modified:April 18, 2006 - v2
Checksum:i455798C8631755A6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti615 – 616GH → PD in CAA52675 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74597 Genomic DNA Translation: CAA52675.1
CP000100 Genomic DNA Translation: ABB57265.1
X61625 Genomic DNA Translation: CAA43813.1
PIRiS36605
RefSeqiWP_011377931.1, NC_007604.1

Genome annotation databases

EnsemblBacteriaiABB57265; ABB57265; Synpcc7942_1235
KEGGisyf:Synpcc7942_1235

Similar proteinsi

Entry informationi

Entry nameiNARB_SYNE7
AccessioniPrimary (citable) accession number: P39458
Secondary accession number(s): Q31NV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 18, 2006
Last modified: April 25, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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