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Protein

Sensor protein TorS

Gene

torS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates TorR via a four-step phosphorelay. When TMAO is removed, TorS can dephosphorylate TorR, probably by a reverse phosphorelay involving His-860 and Asp-733.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Inhibited by TorC apocytochrome.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • intramolecular transferase activity, phosphotransferases Source: EcoCyc
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
  • transferase activity, transferring phosphorus-containing groups Source: EcoCyc

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • cellular response to organic substance Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORS-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein TorS (EC:2.7.13.3)
Gene namesi
Name:torS
Synonyms:yccI
Ordered Locus Names:b0993, JW5135
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12617 torS

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 332PeriplasmicSequence analysisAdd BLAST303
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 914CytoplasmicSequence analysisAdd BLAST561

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi453H → Q: Loss of activity. 1 Publication1
Mutagenesisi733D → A or E: Loss of activity. 1 Publication1
Mutagenesisi860H → Q: Decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748881 – 914Sensor protein TorSAdd BLAST914

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453Phosphohistidine; by autocatalysisCurated1
Modified residuei7334-aspartylphosphateCurated1
Modified residuei860PhosphohistidineCurated1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39453
PRIDEiP39453

PTM databases

iPTMnetiP39453

Interactioni

Subunit structurei

May form homomultimers. Seems to interact with TorT and TorC apocytochrome.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9149134,EBI-9149134

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262839, 4 interactors
DIPiDIP-11017N
IntActiP39453, 1 interactor
STRINGi316385.ECDH10B_1065

Structurei

Secondary structure

1914
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 71Combined sources23
Helixi75 – 100Combined sources26
Turni101 – 103Combined sources3
Helixi107 – 173Combined sources67
Turni177 – 179Combined sources3
Helixi182 – 218Combined sources37
Helixi223 – 227Combined sources5
Helixi229 – 247Combined sources19
Helixi253 – 266Combined sources14
Helixi269 – 313Combined sources45

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I9WX-ray2.70A35-320[»]
ProteinModelPortaliP39453
SMRiP39453
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39453

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 407HAMPPROSITE-ProRule annotationAdd BLAST54
Domaini450 – 664Histidine kinasePROSITE-ProRule annotationAdd BLAST215
Domaini683 – 798Response regulatoryPROSITE-ProRule annotationAdd BLAST116
Domaini821 – 914HPtPROSITE-ProRule annotationAdd BLAST94

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
COG0784 LUCA
COG2198 LUCA
HOGENOMiHOG000118852
InParanoidiP39453
KOiK07647
OMAiIAVSAHV
PhylomeDBiP39453

Family and domain databases

CDDicd06225 HAMP, 1 hit
cd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
cd00088 HPT, 1 hit
cd00156 REC, 1 hit
cd16172 TorS_sensor_domain, 1 hit
Gene3Di1.20.120.160, 1 hit
1.20.58.920, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR036641 HPT_dom_sf
IPR037952 Sensor_TorS
IPR004358 Sig_transdc_His_kin-like_C
IPR008207 Sig_transdc_His_kin_Hpt_dom
IPR014302 Sig_transdc_His_kinase_TorS
IPR001789 Sig_transdc_resp-reg_receiver
IPR038188 TorS_sensor_sf
PANTHERiPTHR43719:SF22 PTHR43719:SF22, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PF01627 Hpt, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF036437 HK_TorS, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00304 HAMP, 1 hit
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SM00073 HPT, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF47226 SSF47226, 1 hit
SSF47384 SSF47384, 1 hit
SSF52172 SSF52172, 1 hit
SSF55874 SSF55874, 1 hit
TIGRFAMsiTIGR02956 TMAO_torS, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit
PS50894 HPT, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P39453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTLTRRLW MGFALMALLT LTSTLVGWYN LRFISQVEKD NTQALIPTMN
60 70 80 90 100
MARQLSEASA WELFAAQNLT SADNEKMWQA QGRMLTAQSL KINALLQALR
110 120 130 140 150
EQGFDTTAIE QQEQEISRSL RQQGELVGQR LQLRQQQQQL SQQIVAAADE
160 170 180 190 200
IARLAQGQAN NATTSAGATQ AGIYDLIEQD QRQAAESALD RLIDIDLEYV
210 220 230 240 250
NQMNELRLSA LRVQQMVMNL GLEQIQKNAP TLEKQLNNAV KILQRRQIRI
260 270 280 290 300
EDPGVRAQVA TTLTTVSQYS DLLALYQQDS EISNHLQTLA QNNIAQFAQF
310 320 330 340 350
SSEVSQLVDT IELRNQHGLA HLEKASARGQ YSLLLLGMVS LCALILILWR
360 370 380 390 400
VVYRSVTRPL AEQTQALQRL LDGDIDSPFP ETAGVRELDT IGRLMDAFRS
410 420 430 440 450
NVHALNRHRE QLAAQVKART AELQELVIEH RQARAEAEKA SQAKSAFLAA
460 470 480 490 500
MSHEIRTPLY GILGTAQLLA DNPALNAQRD DLRAITDSGE SLLTILNDIL
510 520 530 540 550
DYSAIEAGGK NVSVSDEPFE PRPLLESTLQ LMSGRVKGRP IRLATAIADD
560 570 580 590 600
MPCALMGDPR RIRQVITNLL SNALRFTDEG YIILRSRTDG EQWLVEVEDS
610 620 630 640 650
GCGIDPAKLA EIFQPFVQVS GKRGGTGLGL TISSRLAQAM GGELSATSTP
660 670 680 690 700
EVGSCFCLRL PLRVATAPVP KTVNQAVRLD GLRLLLIEDN PLTQRITIEM
710 720 730 740 750
LKTSGAQIVA VGNAAQALET LQNSEPFAAA LVDFDLPDID GITLARQLAQ
760 770 780 790 800
QYPSLVLIGF SAHVIDETLR QRTSSLFRGI IPKPVPREVL GQLLAHYLQL
810 820 830 840 850
QVNNDQSLDV SQLNEDAQLM GTEKIHEWLV LFTQHALPLL DEIDIARASQ
860 870 880 890 900
DSEKIKRAAH QLKSSCSSLG MHIASQLCAQ LEQQPLSAPL PHEEITRSVA
910
ALEAWLHKKD LNAI
Length:914
Mass (Da):101,024
Last modified:August 29, 2001 - v4
Checksum:i45D66BD027B69FCD
GO

Sequence cautioni

The sequence CAA63920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti771 – 772QR → HG in CAA63920 (PubMed:8809780).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti295 – 297Missing in torS726; constitutively active. 3
Natural varianti408H → L in torS729; partial activation. 1
Natural varianti414A → P in torS13; partial activation. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA Translation: CAA63920.1 Different initiation.
U00096 Genomic DNA Translation: AAC74078.2
AP009048 Genomic DNA Translation: BAA36135.2
PIRiG64840
RefSeqiNP_415513.2, NC_000913.3
WP_001300633.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74078; AAC74078; b0993
BAA36135; BAA36135; BAA36135
GeneIDi945595
KEGGiecj:JW5135
eco:b0993
PATRICifig|511145.12.peg.1029

Similar proteinsi

Entry informationi

Entry nameiTORS_ECOLI
AccessioniPrimary (citable) accession number: P39453
Secondary accession number(s): P75887
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 29, 2001
Last modified: March 28, 2018
This is version 169 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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