P39453 (TORS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein torS
EC=2.7.13.3

Gene names

Name:	torS
Synonyms:	yccI
Ordered Locus Names:	b0993, JW5135

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system torS/torR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates torR via a four-step phosphorelay. When TMAO is removed, torS can dephosphorylate torR, probably by a reverse phosphorelay involving His-860 and Asp-733.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Enzyme regulation	Inhibited by torC apocytochrome.
Subunit structure	May form homomultimers. Seems to interact with torT and torC apocytochrome.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.8.
Post-translational modification	Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 HPt domain. Contains 1 response regulatory domain.
Sequence caution	The sequence CAA63920.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anaerobic respiration Inferred from mutant phenotype Ref.1. Source: EcoCyc cellular response to organic substance Inferred from mutant phenotype Ref.1. Source: EcoCyc peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro protein autophosphorylation Inferred from mutant phenotype Ref.6. Source: EcoCyc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW intramolecular transferase activity, phosphotransferases Inferred from mutant phenotype Ref.6. Source: EcoCyc phosphoprotein phosphatase activity Inferred from mutant phenotype Ref.7. Source: EcoCyc protein homodimerization activity Inferred from mutant phenotype Ref.6. Source: EcoCyc two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from mutant phenotype Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 914

914

Sensor protein torS

PRO_0000074888

Regions

Topological domain

1 – 8

8

Cytoplasmic Potential

Transmembrane

9 – 29

21

Helical; Potential

Topological domain

30 – 332

303

Periplasmic Potential

Transmembrane

333 – 353

21

Helical; Potential

Topological domain

354 – 914

561

Cytoplasmic Potential

Domain

354 – 407

54

HAMP

Domain

450 – 664

215

Histidine kinase

Domain

683 – 798

116

Response regulatory

Domain

821 – 914

94

HPt

Amino acid modifications

Modified residue

453

1

Phosphohistidine; by autocatalysis Probable

Modified residue

733

1

4-aspartylphosphate Probable

Modified residue

860

1

Phosphohistidine Probable

Natural variations

Natural variant

295 – 297

3

Missing in torS726; constitutively active.

Natural variant

408

1

H → L in torS729; partial activation.

Natural variant

414

1

A → P in torS13; partial activation.

Experimental info

Mutagenesis

453

1

H → Q: Loss of activity. Ref.6

Mutagenesis

733

1

D → A or E: Loss of activity. Ref.6

Mutagenesis

860

1

H → Q: Decrease in activity. Ref.6

Sequence conflict

771 – 772

2

QR → HG in CAA63920. Ref.1

Secondary structure

1

.

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914

Helix Strand Turn

Details...

Helix

49 – 71

23

Helix

75 – 80

6

Helix

84 – 100

17

Helix

107 – 122

16

Helix

130 – 155

26

Helix

159 – 165

7

Turn

177 – 179

3

Helix

182 – 218

37

Helix

223 – 227

5

Helix

229 – 247

19

Helix

258 – 266

9

Helix

269 – 313

45

Sequences

	Sequence		Length	Mass (Da)
	P39453 [UniParc]. Last modified August 29, 2001. Version 4. Checksum: 45D66BD027B69FCD	FASTA	914	101,024
	10 20 30 40 50 60 MNLTLTRRLW MGFALMALLT LTSTLVGWYN LRFISQVEKD NTQALIPTMN MARQLSEASA 70 80 90 100 110 120 WELFAAQNLT SADNEKMWQA QGRMLTAQSL KINALLQALR EQGFDTTAIE QQEQEISRSL 130 140 150 160 170 180 RQQGELVGQR LQLRQQQQQL SQQIVAAADE IARLAQGQAN NATTSAGATQ AGIYDLIEQD 190 200 210 220 230 240 QRQAAESALD RLIDIDLEYV NQMNELRLSA LRVQQMVMNL GLEQIQKNAP TLEKQLNNAV 250 260 270 280 290 300 KILQRRQIRI EDPGVRAQVA TTLTTVSQYS DLLALYQQDS EISNHLQTLA QNNIAQFAQF 310 320 330 340 350 360 SSEVSQLVDT IELRNQHGLA HLEKASARGQ YSLLLLGMVS LCALILILWR VVYRSVTRPL 370 380 390 400 410 420 AEQTQALQRL LDGDIDSPFP ETAGVRELDT IGRLMDAFRS NVHALNRHRE QLAAQVKART 430 440 450 460 470 480 AELQELVIEH RQARAEAEKA SQAKSAFLAA MSHEIRTPLY GILGTAQLLA DNPALNAQRD 490 500 510 520 530 540 DLRAITDSGE SLLTILNDIL DYSAIEAGGK NVSVSDEPFE PRPLLESTLQ LMSGRVKGRP 550 560 570 580 590 600 IRLATAIADD MPCALMGDPR RIRQVITNLL SNALRFTDEG YIILRSRTDG EQWLVEVEDS 610 620 630 640 650 660 GCGIDPAKLA EIFQPFVQVS GKRGGTGLGL TISSRLAQAM GGELSATSTP EVGSCFCLRL 670 680 690 700 710 720 PLRVATAPVP KTVNQAVRLD GLRLLLIEDN PLTQRITIEM LKTSGAQIVA VGNAAQALET 730 740 750 760 770 780 LQNSEPFAAA LVDFDLPDID GITLARQLAQ QYPSLVLIGF SAHVIDETLR QRTSSLFRGI 790 800 810 820 830 840 IPKPVPREVL GQLLAHYLQL QVNNDQSLDV SQLNEDAQLM GTEKIHEWLV LFTQHALPLL 850 860 870 880 890 900 DEIDIARASQ DSEKIKRAAH QLKSSCSSLG MHIASQLCAQ LEQQPLSAPL PHEEITRSVA 910 ALEAWLHKKD LNAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An unorthodox sensor protein (TorS) mediates the induction of the tor structural genes in response to trimethylamine N-oxide in Escherichia coli." Jourlin C., Bengrine A., Chippaux M., Mejean V. Mol. Microbiol. 20:1297-1306(1996) [PubMed: 8809780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TORS13; TORS726 AND TORS729. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes." Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C. J. Bacteriol. 176:5601-5606(1994) [PubMed: 8083154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[6]	"Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli." Jourlin C., Ansaldi M., Mejean V. J. Mol. Biol. 267:770-777(1997) [PubMed: 9135110] [Abstract] Cited for: MUTAGENESIS OF HIS-453; ASP-733 AND HIS-860.
[7]	"Rapid dephosphorylation of the TorR response regulator by the TorS unorthodox sensor in Escherichia coli." Ansaldi M., Jourlin-Castelli C., Lepelletier M., Theraulaz L., Mejean V. J. Bacteriol. 183:2691-2695(2001) [PubMed: 11274133] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X94231 Genomic DNA. Translation: CAA63920.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74078.2.
AP009048 Genomic DNA. Translation: BAA36135.2.

PIR

G64840.

RefSeq

NP_415513.2. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I9W	X-ray	2.70	A	35-320	[»]

ProteinModelPortal

P39453.

SMR

P39453. Positions 47-316, 436-663, 680-800, 821-891.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-11017N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002836; EBESCP00000002836; EBESCG00000002313.
EBESCT00000017695; EBESCP00000016986; EBESCG00000016751.

GeneID

945595.

GenomeReviews

Gene locus JW5135 in contig AP009048_GR.
Gene locus b0993 in contig U00096_GR.

KEGG

ecj:JW5135.
eco:b0993.

PATRIC

32117211. VBIEscCol129921_1029.

Organism-specific databases

EchoBASE

EB2501.

EcoGene

EG12617. torS.

Phylogenomic databases

eggNOG

COG0642.

GeneTree

EBGT00070000031695.

HOGENOM

HBG466899.

OMA

HNARAML.

PhylomeDB

P39453.

ProtClustDB

PRK11466.

Enzyme and pathway databases

BioCyc

EcoCyc:TORS-MONOMER.

BRENDA

2.7.13.3. 2026.

Gene expression databases

Genevestigator

P39453.

Family and domain databases

InterPro

IPR003594. ATPase-like_ATP-bd.
IPR011006. CheY-like_superfamily.
IPR003660. HAMP_linker_domain.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR014302. Sig_transdc_His_kinase_TorS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:1.20.120.160. Sig_transdc_His_kin_Hpt_dom. 1 hit.

KO

K07647.

Pfam

PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]

PIRSF

PIRSF036437. HK_TorS. 1 hit.

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.
SSF52172. CheY_like. 1 hit.
SSF47384. His_kin_homodim. 1 hit.
SSF47226. Hpt. 1 hit.

TIGRFAMs

TIGR02956. TMAO_torS. 1 hit.

PROSITE

PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TORS_ECOLI

Accession

Primary (citable) accession number: P39453
Secondary accession number(s): P75887

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 119 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program