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Protein

Sensor protein TorS

Gene

torS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates TorR via a four-step phosphorelay. When TMAO is removed, TorS can dephosphorylate TorR, probably by a reverse phosphorelay involving His-860 and Asp-733.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Inhibited by TorC apocytochrome.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • intramolecular transferase activity, phosphotransferases Source: EcoCyc
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
  • transferase activity, transferring phosphorus-containing groups Source: EcoCyc

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • cellular response to organic substance Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORS-MONOMER.
ECOL316407:JW5135-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein TorS (EC:2.7.13.3)
Gene namesi
Name:torS
Synonyms:yccI
Ordered Locus Names:b0993, JW5135
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12617. torS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 332PeriplasmicSequence analysisAdd BLAST303
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 914CytoplasmicSequence analysisAdd BLAST561

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi453H → Q: Loss of activity. 1 Publication1
Mutagenesisi733D → A or E: Loss of activity. 1 Publication1
Mutagenesisi860H → Q: Decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748881 – 914Sensor protein TorSAdd BLAST914

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453Phosphohistidine; by autocatalysisCurated1
Modified residuei7334-aspartylphosphateCurated1
Modified residuei860PhosphohistidineCurated1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39453.
PRIDEiP39453.

Interactioni

Subunit structurei

May form homomultimers. Seems to interact with TorT and TorC apocytochrome.

Protein-protein interaction databases

BioGridi4262839. 3 interactors.
DIPiDIP-11017N.
IntActiP39453. 1 interactor.
STRINGi511145.b0993.

Structurei

Secondary structure

1914
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 71Combined sources23
Helixi75 – 100Combined sources26
Turni101 – 103Combined sources3
Helixi107 – 173Combined sources67
Turni177 – 179Combined sources3
Helixi182 – 218Combined sources37
Helixi223 – 227Combined sources5
Helixi229 – 247Combined sources19
Helixi253 – 266Combined sources14
Helixi269 – 313Combined sources45

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I9WX-ray2.70A35-320[»]
ProteinModelPortaliP39453.
SMRiP39453.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39453.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 407HAMPPROSITE-ProRule annotationAdd BLAST54
Domaini450 – 664Histidine kinasePROSITE-ProRule annotationAdd BLAST215
Domaini683 – 798Response regulatoryPROSITE-ProRule annotationAdd BLAST116
Domaini821 – 914HPtPROSITE-ProRule annotationAdd BLAST94

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 HPt domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000118852.
InParanoidiP39453.
KOiK07647.
OMAiIAVSAHV.
PhylomeDBiP39453.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014302. Sig_transdc_His_kinase_TorS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF036437. HK_TorS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02956. TMAO_torS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTLTRRLW MGFALMALLT LTSTLVGWYN LRFISQVEKD NTQALIPTMN
60 70 80 90 100
MARQLSEASA WELFAAQNLT SADNEKMWQA QGRMLTAQSL KINALLQALR
110 120 130 140 150
EQGFDTTAIE QQEQEISRSL RQQGELVGQR LQLRQQQQQL SQQIVAAADE
160 170 180 190 200
IARLAQGQAN NATTSAGATQ AGIYDLIEQD QRQAAESALD RLIDIDLEYV
210 220 230 240 250
NQMNELRLSA LRVQQMVMNL GLEQIQKNAP TLEKQLNNAV KILQRRQIRI
260 270 280 290 300
EDPGVRAQVA TTLTTVSQYS DLLALYQQDS EISNHLQTLA QNNIAQFAQF
310 320 330 340 350
SSEVSQLVDT IELRNQHGLA HLEKASARGQ YSLLLLGMVS LCALILILWR
360 370 380 390 400
VVYRSVTRPL AEQTQALQRL LDGDIDSPFP ETAGVRELDT IGRLMDAFRS
410 420 430 440 450
NVHALNRHRE QLAAQVKART AELQELVIEH RQARAEAEKA SQAKSAFLAA
460 470 480 490 500
MSHEIRTPLY GILGTAQLLA DNPALNAQRD DLRAITDSGE SLLTILNDIL
510 520 530 540 550
DYSAIEAGGK NVSVSDEPFE PRPLLESTLQ LMSGRVKGRP IRLATAIADD
560 570 580 590 600
MPCALMGDPR RIRQVITNLL SNALRFTDEG YIILRSRTDG EQWLVEVEDS
610 620 630 640 650
GCGIDPAKLA EIFQPFVQVS GKRGGTGLGL TISSRLAQAM GGELSATSTP
660 670 680 690 700
EVGSCFCLRL PLRVATAPVP KTVNQAVRLD GLRLLLIEDN PLTQRITIEM
710 720 730 740 750
LKTSGAQIVA VGNAAQALET LQNSEPFAAA LVDFDLPDID GITLARQLAQ
760 770 780 790 800
QYPSLVLIGF SAHVIDETLR QRTSSLFRGI IPKPVPREVL GQLLAHYLQL
810 820 830 840 850
QVNNDQSLDV SQLNEDAQLM GTEKIHEWLV LFTQHALPLL DEIDIARASQ
860 870 880 890 900
DSEKIKRAAH QLKSSCSSLG MHIASQLCAQ LEQQPLSAPL PHEEITRSVA
910
ALEAWLHKKD LNAI
Length:914
Mass (Da):101,024
Last modified:August 29, 2001 - v4
Checksum:i45D66BD027B69FCD
GO

Sequence cautioni

The sequence CAA63920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti771 – 772QR → HG in CAA63920 (PubMed:8809780).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti295 – 297Missing in torS726; constitutively active. 3
Natural varianti408H → L in torS729; partial activation. 1
Natural varianti414A → P in torS13; partial activation. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63920.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74078.2.
AP009048 Genomic DNA. Translation: BAA36135.2.
PIRiG64840.
RefSeqiNP_415513.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74078; AAC74078; b0993.
BAA36135; BAA36135; BAA36135.
GeneIDi945595.
KEGGiecj:JW5135.
eco:b0993.
PATRICi32117211. VBIEscCol129921_1029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63920.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74078.2.
AP009048 Genomic DNA. Translation: BAA36135.2.
PIRiG64840.
RefSeqiNP_415513.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I9WX-ray2.70A35-320[»]
ProteinModelPortaliP39453.
SMRiP39453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262839. 3 interactors.
DIPiDIP-11017N.
IntActiP39453. 1 interactor.
STRINGi511145.b0993.

Proteomic databases

PaxDbiP39453.
PRIDEiP39453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74078; AAC74078; b0993.
BAA36135; BAA36135; BAA36135.
GeneIDi945595.
KEGGiecj:JW5135.
eco:b0993.
PATRICi32117211. VBIEscCol129921_1029.

Organism-specific databases

EchoBASEiEB2501.
EcoGeneiEG12617. torS.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000118852.
InParanoidiP39453.
KOiK07647.
OMAiIAVSAHV.
PhylomeDBiP39453.

Enzyme and pathway databases

BioCyciEcoCyc:TORS-MONOMER.
ECOL316407:JW5135-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP39453.
PROiP39453.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014302. Sig_transdc_His_kinase_TorS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF036437. HK_TorS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02956. TMAO_torS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTORS_ECOLI
AccessioniPrimary (citable) accession number: P39453
Secondary accession number(s): P75887
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 29, 2001
Last modified: November 2, 2016
This is version 158 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.