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Protein

Sensor protein TorS

Gene

torS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TorS/TorR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates TorR via a four-step phosphorelay. When TMAO is removed, TorS can dephosphorylate TorR, probably by a reverse phosphorelay involving His-860 and Asp-733.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Inhibited by TorC apocytochrome.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • intramolecular transferase activity, phosphotransferases Source: EcoCyc
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
  • transferase activity, transferring phosphorus-containing groups Source: EcoCyc

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • cellular response to organic substance Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:TORS-MONOMER.
ECOL316407:JW5135-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein TorS (EC:2.7.13.3)
Gene namesi
Name:torS
Synonyms:yccI
Ordered Locus Names:b0993, JW5135
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12617. torS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 332303PeriplasmicSequence analysisAdd
BLAST
Transmembranei333 – 35321HelicalSequence analysisAdd
BLAST
Topological domaini354 – 914561CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi453 – 4531H → Q: Loss of activity. 1 Publication
Mutagenesisi733 – 7331D → A or E: Loss of activity. 1 Publication
Mutagenesisi860 – 8601H → Q: Decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Sensor protein TorSPRO_0000074888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei453 – 4531Phosphohistidine; by autocatalysisCurated
Modified residuei733 – 73314-aspartylphosphateCurated
Modified residuei860 – 8601PhosphohistidineCurated

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39453.
PRIDEiP39453.

Interactioni

Subunit structurei

May form homomultimers. Seems to interact with TorT and TorC apocytochrome.

Protein-protein interaction databases

BioGridi4262839. 3 interactions.
DIPiDIP-11017N.
IntActiP39453. 1 interaction.
STRINGi511145.b0993.

Structurei

Secondary structure

1
914
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi49 – 7123Combined sources
Helixi75 – 10026Combined sources
Turni101 – 1033Combined sources
Helixi107 – 17367Combined sources
Turni177 – 1793Combined sources
Helixi182 – 21837Combined sources
Helixi223 – 2275Combined sources
Helixi229 – 24719Combined sources
Helixi253 – 26614Combined sources
Helixi269 – 31345Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I9WX-ray2.70A35-320[»]
ProteinModelPortaliP39453.
SMRiP39453. Positions 47-316, 415-661, 680-799.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39453.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini354 – 40754HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini450 – 664215Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini683 – 798116Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini821 – 91494HPtPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 HPt domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000118852.
InParanoidiP39453.
KOiK07647.
OMAiIAVSAHV.
OrthoDBiEOG6G4VQG.
PhylomeDBiP39453.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014302. Sig_transdc_His_kinase_TorS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF036437. HK_TorS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02956. TMAO_torS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTLTRRLW MGFALMALLT LTSTLVGWYN LRFISQVEKD NTQALIPTMN
60 70 80 90 100
MARQLSEASA WELFAAQNLT SADNEKMWQA QGRMLTAQSL KINALLQALR
110 120 130 140 150
EQGFDTTAIE QQEQEISRSL RQQGELVGQR LQLRQQQQQL SQQIVAAADE
160 170 180 190 200
IARLAQGQAN NATTSAGATQ AGIYDLIEQD QRQAAESALD RLIDIDLEYV
210 220 230 240 250
NQMNELRLSA LRVQQMVMNL GLEQIQKNAP TLEKQLNNAV KILQRRQIRI
260 270 280 290 300
EDPGVRAQVA TTLTTVSQYS DLLALYQQDS EISNHLQTLA QNNIAQFAQF
310 320 330 340 350
SSEVSQLVDT IELRNQHGLA HLEKASARGQ YSLLLLGMVS LCALILILWR
360 370 380 390 400
VVYRSVTRPL AEQTQALQRL LDGDIDSPFP ETAGVRELDT IGRLMDAFRS
410 420 430 440 450
NVHALNRHRE QLAAQVKART AELQELVIEH RQARAEAEKA SQAKSAFLAA
460 470 480 490 500
MSHEIRTPLY GILGTAQLLA DNPALNAQRD DLRAITDSGE SLLTILNDIL
510 520 530 540 550
DYSAIEAGGK NVSVSDEPFE PRPLLESTLQ LMSGRVKGRP IRLATAIADD
560 570 580 590 600
MPCALMGDPR RIRQVITNLL SNALRFTDEG YIILRSRTDG EQWLVEVEDS
610 620 630 640 650
GCGIDPAKLA EIFQPFVQVS GKRGGTGLGL TISSRLAQAM GGELSATSTP
660 670 680 690 700
EVGSCFCLRL PLRVATAPVP KTVNQAVRLD GLRLLLIEDN PLTQRITIEM
710 720 730 740 750
LKTSGAQIVA VGNAAQALET LQNSEPFAAA LVDFDLPDID GITLARQLAQ
760 770 780 790 800
QYPSLVLIGF SAHVIDETLR QRTSSLFRGI IPKPVPREVL GQLLAHYLQL
810 820 830 840 850
QVNNDQSLDV SQLNEDAQLM GTEKIHEWLV LFTQHALPLL DEIDIARASQ
860 870 880 890 900
DSEKIKRAAH QLKSSCSSLG MHIASQLCAQ LEQQPLSAPL PHEEITRSVA
910
ALEAWLHKKD LNAI
Length:914
Mass (Da):101,024
Last modified:August 29, 2001 - v4
Checksum:i45D66BD027B69FCD
GO

Sequence cautioni

The sequence CAA63920.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti771 – 7722QR → HG in CAA63920 (PubMed:8809780).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti295 – 2973Missing in torS726; constitutively active.
Natural varianti408 – 4081H → L in torS729; partial activation.
Natural varianti414 – 4141A → P in torS13; partial activation.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63920.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74078.2.
AP009048 Genomic DNA. Translation: BAA36135.2.
PIRiG64840.
RefSeqiNP_415513.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74078; AAC74078; b0993.
BAA36135; BAA36135; BAA36135.
GeneIDi945595.
KEGGiecj:JW5135.
eco:b0993.
PATRICi32117211. VBIEscCol129921_1029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94231 Genomic DNA. Translation: CAA63920.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74078.2.
AP009048 Genomic DNA. Translation: BAA36135.2.
PIRiG64840.
RefSeqiNP_415513.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I9WX-ray2.70A35-320[»]
ProteinModelPortaliP39453.
SMRiP39453. Positions 47-316, 415-661, 680-799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262839. 3 interactions.
DIPiDIP-11017N.
IntActiP39453. 1 interaction.
STRINGi511145.b0993.

Proteomic databases

PaxDbiP39453.
PRIDEiP39453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74078; AAC74078; b0993.
BAA36135; BAA36135; BAA36135.
GeneIDi945595.
KEGGiecj:JW5135.
eco:b0993.
PATRICi32117211. VBIEscCol129921_1029.

Organism-specific databases

EchoBASEiEB2501.
EcoGeneiEG12617. torS.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
HOGENOMiHOG000118852.
InParanoidiP39453.
KOiK07647.
OMAiIAVSAHV.
OrthoDBiEOG6G4VQG.
PhylomeDBiP39453.

Enzyme and pathway databases

BioCyciEcoCyc:TORS-MONOMER.
ECOL316407:JW5135-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP39453.
PROiP39453.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR014302. Sig_transdc_His_kinase_TorS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF036437. HK_TorS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02956. TMAO_torS. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An unorthodox sensor protein (TorS) mediates the induction of the tor structural genes in response to trimethylamine N-oxide in Escherichia coli."
    Jourlin C., Bengrine A., Chippaux M., Mejean V.
    Mol. Microbiol. 20:1297-1306(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TORS13; TORS726 AND TORS729.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes."
    Simon G., Mejean V., Jourlin C., Chippaux M., Pascal M.-C.
    J. Bacteriol. 176:5601-5606(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. "Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli."
    Jourlin C., Ansaldi M., Mejean V.
    J. Mol. Biol. 267:770-777(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-453; ASP-733 AND HIS-860.
  7. "Rapid dephosphorylation of the TorR response regulator by the TorS unorthodox sensor in Escherichia coli."
    Ansaldi M., Jourlin-Castelli C., Lepelletier M., Theraulaz L., Mejean V.
    J. Bacteriol. 183:2691-2695(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiTORS_ECOLI
AccessioniPrimary (citable) accession number: P39453
Secondary accession number(s): P75887
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 29, 2001
Last modified: July 6, 2016
This is version 155 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.