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Protein

Alcohol dehydrogenase, propanol-preferring

Gene

adhP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Preferred specificity is towards 1-propanol.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Zinc 1; catalyticBy similarity
Metal bindingi58 – 581Zinc 1; catalyticBy similarity
Metal bindingi89 – 891Zinc 2By similarity
Metal bindingi92 – 921Zinc 2By similarity
Metal bindingi95 – 951Zinc 2By similarity
Metal bindingi103 – 1031Zinc 2By similarity
Metal bindingi145 – 1451Zinc 1; catalyticBy similarity

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • acetaldehyde catabolic process Source: EcoliWiki
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • response to ethanol Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ADHP-MONOMER.
ECOL316407:JW1474-MONOMER.
MetaCyc:ADHP-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase, propanol-preferring (EC:1.1.1.1)
Gene namesi
Name:adhP
Synonyms:yddN
Ordered Locus Names:b1478, JW1474
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12622. adhP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Alcohol dehydrogenase, propanol-preferringPRO_0000160740Add
BLAST

Proteomic databases

EPDiP39451.
PaxDbiP39451.
PRIDEiP39451.

Interactioni

Protein-protein interaction databases

BioGridi4262899. 10 interactions.
IntActiP39451. 5 interactions.
STRINGi511145.b1478.

Structurei

Secondary structure

1
336
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Beta strandi12 – 165Combined sources
Beta strandi26 – 3510Combined sources
Helixi38 – 458Combined sources
Turni46 – 483Combined sources
Beta strandi58 – 6710Combined sources
Beta strandi79 – 879Combined sources
Beta strandi90 – 923Combined sources
Helixi95 – 973Combined sources
Helixi100 – 1023Combined sources
Turni109 – 1113Combined sources
Beta strandi116 – 1249Combined sources
Helixi125 – 1273Combined sources
Helixi137 – 1437Combined sources
Helixi146 – 15611Combined sources
Beta strandi164 – 1685Combined sources
Helixi172 – 18312Combined sources
Beta strandi188 – 1947Combined sources
Helixi196 – 2049Combined sources
Beta strandi208 – 2125Combined sources
Turni213 – 2153Combined sources
Helixi218 – 2269Combined sources
Beta strandi227 – 2348Combined sources
Helixi239 – 24810Combined sources
Beta strandi249 – 2579Combined sources
Beta strandi262 – 2687Combined sources
Helixi269 – 2746Combined sources
Beta strandi278 – 2814Combined sources
Helixi287 – 29812Combined sources
Beta strandi306 – 3094Combined sources
Helixi311 – 3133Combined sources
Helixi314 – 3229Combined sources
Beta strandi327 – 3337Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GKVX-ray2.01A/B/C/D1-336[»]
ProteinModelPortaliP39451.
SMRiP39451. Positions 1-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DQ4. Bacteria.
COG1064. LUCA.
HOGENOMiHOG000294685.
InParanoidiP39451.
KOiK13953.
OMAiATHCIVS.
PhylomeDBiP39451.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39451-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAVVTKDH HVDVTYKTLR SLKHGEALLK MECCGVCHTD LHVKNGDFGD
60 70 80 90 100
KTGVILGHEG IGVVAEVGPG VTSLKPGDRA SVAWFYEGCG HCEYCNSGNE
110 120 130 140 150
TLCRSVKNAG YSVDGGMAEE CIVVADYAVK VPDGLDSAAA SSITCAGVTT
160 170 180 190 200
YKAVKLSKIR PGQWIAIYGL GGLGNLALQY AKNVFNAKVI AIDVNDEQLK
210 220 230 240 250
LATEMGADLA INSHTEDAAK IVQEKTGGAH AAVVTAVAKA AFNSAVDAVR
260 270 280 290 300
AGGRVVAVGL PPESMSLDIP RLVLDGIEVV GSLVGTRQDL TEAFQFAAEG
310 320 330
KVVPKVALRP LADINTIFTE MEEGKIRGRM VIDFRH
Length:336
Mass (Da):35,380
Last modified:November 1, 1997 - v1
Checksum:i8A9080F273866947
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341C → P AA sequence (Ref. 4) Curated
Sequence conflicti90 – 901G → R in M31532 (PubMed:2158980).Curated
Sequence conflicti104 – 1041R → P in M31532 (PubMed:2158980).Curated
Sequence conflicti108 – 1081N → K in M31532 (PubMed:2158980).Curated
Sequence conflicti112 – 1121S → G in M31532 (PubMed:2158980).Curated
Sequence conflicti117 – 1182MA → RV in M31532 (PubMed:2158980).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74551.2.
AP009048 Genomic DNA. Translation: BAA15126.1.
M31532 Genomic DNA. No translation available.
PIRiA64901.
RefSeqiNP_415995.4. NC_000913.3.
WP_000642433.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74551; AAC74551; b1478.
BAA15126; BAA15126; BAA15126.
GeneIDi946036.
KEGGiecj:JW1474.
eco:b1478.
PATRICi32118250. VBIEscCol129921_1545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74551.2.
AP009048 Genomic DNA. Translation: BAA15126.1.
M31532 Genomic DNA. No translation available.
PIRiA64901.
RefSeqiNP_415995.4. NC_000913.3.
WP_000642433.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GKVX-ray2.01A/B/C/D1-336[»]
ProteinModelPortaliP39451.
SMRiP39451. Positions 1-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262899. 10 interactions.
IntActiP39451. 5 interactions.
STRINGi511145.b1478.

Proteomic databases

EPDiP39451.
PaxDbiP39451.
PRIDEiP39451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74551; AAC74551; b1478.
BAA15126; BAA15126; BAA15126.
GeneIDi946036.
KEGGiecj:JW1474.
eco:b1478.
PATRICi32118250. VBIEscCol129921_1545.

Organism-specific databases

EchoBASEiEB2506.
EcoGeneiEG12622. adhP.

Phylogenomic databases

eggNOGiENOG4105DQ4. Bacteria.
COG1064. LUCA.
HOGENOMiHOG000294685.
InParanoidiP39451.
KOiK13953.
OMAiATHCIVS.
PhylomeDBiP39451.

Enzyme and pathway databases

BioCyciEcoCyc:ADHP-MONOMER.
ECOL316407:JW1474-MONOMER.
MetaCyc:ADHP-MONOMER.

Miscellaneous databases

PROiP39451.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADHP_ECOLI
AccessioniPrimary (citable) accession number: P39451
Secondary accession number(s): P76126, P78157, P78268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.