P39451 (ADHP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase, propanol-preferring
EC=1.1.1.1

Gene names

Name:	adhP
Synonyms:	yddN
Ordered Locus Names:	b1478, JW1474

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Preferred specificity is towards 1-propanol.
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Alcohol dehydrogenase, propanol-preferring		PRO_0000160740
Sites
	Metal binding	37	1	Zinc 1; catalytic By similarity
	Metal binding	58	1	Zinc 1; catalytic By similarity
	Metal binding	89	1	Zinc 2 By similarity
	Metal binding	92	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	145	1	Zinc 1; catalytic By similarity
Experimental info
	Sequence conflict	34	1	C → P AA sequence Ref.4
	Sequence conflict	90	1	G → R in M31532. Ref.5
	Sequence conflict	104	1	R → P in M31532. Ref.5
	Sequence conflict	108	1	N → K in M31532. Ref.5
	Sequence conflict	112	1	S → G in M31532. Ref.5
	Sequence conflict	117 – 118	2	MA → RV in M31532. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P39451 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8A9080F273866947	FASTA	336	35,380
	10 20 30 40 50 60 MKAAVVTKDH HVDVTYKTLR SLKHGEALLK MECCGVCHTD LHVKNGDFGD KTGVILGHEG 70 80 90 100 110 120 IGVVAEVGPG VTSLKPGDRA SVAWFYEGCG HCEYCNSGNE TLCRSVKNAG YSVDGGMAEE 130 140 150 160 170 180 CIVVADYAVK VPDGLDSAAA SSITCAGVTT YKAVKLSKIR PGQWIAIYGL GGLGNLALQY 190 200 210 220 230 240 AKNVFNAKVI AIDVNDEQLK LATEMGADLA INSHTEDAAK IVQEKTGGAH AAVVTAVAKA 250 260 270 280 290 300 AFNSAVDAVR AGGRVVAVGL PPESMSLDIP RLVLDGIEVV GSLVGTRQDL TEAFQFAAEG 310 320 330 KVVPKVALRP LADINTIFTE MEEGKIRGRM VIDFRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Harayama S. Submitted (OCT-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-35, CHARACTERIZATION.
[5]	"Bacterial transposon Tn7 utilizes two different classes of target sites." Kubo K.M., Craig N.L. J. Bacteriol. 172:2774-2778(1990) [PubMed: 2158980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 87-118.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74551.2. AP009048 Genomic DNA. Translation: BAA15126.1. M31532 Genomic DNA. No translation available.
PIR	A64901.
RefSeq	NP_415995.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P39451.
SMR	P39451. Positions 1-336.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39451. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004089; EBESCP00000004089; EBESCG00000003340. EBESCT00000015957; EBESCP00000015248; EBESCG00000015017.
GeneID	946036.
GenomeReviews	Gene locus JW1474 in contig AP009048_GR. Gene locus b1478 in contig U00096_GR.
KEGG	ecj:JW1474. eco:b1478.
PATRIC	32118250. VBIEscCol129921_1545.
Organism-specific databases
EchoBASE	EB2506.
EcoGene	EG12622. adhP.
Phylogenomic databases
eggNOG	COG1064.
GeneTree	EBGT00050000008796.
HOGENOM	HBG753318.
OMA	VRPGQWI.
PhylomeDB	P39451.
ProtClustDB	PRK09422.
Enzyme and pathway databases
BioCyc	EcoCyc:ADHP-MONOMER. MetaCyc:ADHP-MONOMER.
Gene expression databases
Genevestigator	P39451.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K13953.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADHP_ECOLI

Accession

Primary (citable) accession number: P39451
Secondary accession number(s): P76126, P78157, P78268

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program