P39450 (FRMA_PASPI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase
EC=1.1.1.284

Alternative name(s):
Alcohol dehydrogenase class-3
EC=1.1.1.1
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase
Short name=FALDH
Short name=FDH
Short name=GSH-FDH
EC=1.1.1.-

Gene names

Name:

frmA

Encoded on

Plasmid

Organism

Pasteurella piscicida (Photobacterium damsela subsp. piscicida)

Taxonomic identifier

38294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H. An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
Sequence caution	The sequence BAA03719.1 differs from that shown. Reason: Frameshift at position 128.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	ethanol oxidation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	S-(hydroxymethyl)glutathione dehydrogenase activity Inferred from electronic annotation. Source: EC alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	S-(hydroxymethyl)glutathione dehydrogenase		PRO_0000160777
Sites
	Metal binding	40	1	Zinc 1; catalytic By similarity
	Metal binding	62	1	Zinc 1; catalytic By similarity
	Metal binding	92	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 2 By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	106	1	Zinc 2 By similarity
	Metal binding	169	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39450 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: ACAEA4581048901C	FASTA	369	39,094
	10 20 30 40 50 60 MKSRAAVAFG PGLPLEIVEI DVAPPKKGEV LVKISHTGVC HTDAYTLSGD DPEGLFPVVL 70 80 90 100 110 120 GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECDFCTS GKTNLCVAVR ETQGKGVMPD 130 140 150 160 170 180 ATSRFFVNGQ PLYHYMGCST FSEYTVVAEV SLAKINPQAN AEQVCLLGCG VTTGIGAVHN 190 200 210 220 230 240 TAKVQEGDSV AVFGLGGIGL AVVQGARQAK AGRIFAIDTN PSKFELAKQF GATDCINPND 250 260 270 280 290 300 YDKPVQQVLV EMTKWGVDHT FECIGNVNVM RSALESAHRG WGQSVIIGVA GAGKEISTRP 310 320 330 340 350 360 FQLVTGRVWK GTAFGGVKGR TQLPGMVEDA MSGKIELAPF VTHTMELDKI NEAFDLMHDG KSIRTVIHY

References

[1]	"The transposon-like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida." Kim E.H., Aoki T. Microbiol. Immunol. 38:31-38(1994) [PubMed: 8052160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Rudd K.E., Bairoch A. Unpublished observations (NOV-1994) Cited for: IDENTIFICATION, CONCEPTUAL TRANSLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16172 Genomic DNA. Translation: BAA03719.1. Frameshift.
RefSeq	YP_003023972.1. NC_012919.1. YP_908417.1. NC_008612.1. YP_908601.1. NC_008613.1.
3D structure databases
ProteinModelPortal	P39450.
SMR	P39450. Positions 1-367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4549487. 4549496. 8242395.
Phylogenomic databases
ProtClustDB	CLSK861793.
Family and domain databases
InterPro	IPR014183. ADH_3. IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
TIGRFAMs	TIGR02818. Adh_III_F_hyde. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRMA_PASPI

Accession

Primary (citable) accession number: P39450

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program