Tight junction protein ZO-1 - Mus musculus (Mouse)

Sequence

>sp|P39447|ZO1_MOUSE Tight junction protein ZO-1 OS=Mus musculus GN=Tjp1 PE=1 SV=1 MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVL KGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPD PEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQ PAKPTKVTLVKSRKNEEYGLRPASHIFVKEISQDSLAARDGDIQEGDVVLKINGTVTENM SLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHS GRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAISTPVKHVD DHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRP SMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIR EEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKG EVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRAD FWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREK LAREEPDIYQIAKSELRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQ WYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMND GWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHT SDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSP QAQPQAIHRIDSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPA PAPPTSHASQPGCLGAPSAEAAHVGLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILK QPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYRYEVSSYTDQFSRNYDHRLRF EDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQRFEEPAPLSYD SRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQ VPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLRSATKPQPPPPTLTEEEEDPAMKP QSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDK TLYRLPEPQKPQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHS EPAKPVHSQSQPNFPSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPL SSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKS FPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPEL SSKTPTSPKTLMKAHSSTQPPEFGSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEE DEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILP PLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSV LIDHF

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Reviewed, UniProtKB/Swiss-Prot P39447 (ZO1_MOUSE)

Last modified November 25, 2008. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Tight junction protein ZO-1
Alternative name(s):
    Zonula occludens protein 1
    Zona occludens protein 1
    Tight junction protein 1

Gene names

Name:	Tjp1
Synonyms:	Zo1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1745 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
Subunit structure	Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CXADR, GJD3 and HSPA4 By similarity. Interacts with occludin, claudins, GJA12 and KIRREL1.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic sideBy similarity. Cell junction › tight junctionBy similarity. Note= Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact By similarity.
Domain	The second PDZ domain mediates interaction with GJA12.
Sequence similarities	Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. Contains 1 ZU5 domain.

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Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cell-cell adherens junction Inferred from direct assay. Source: MGI cytoplasm Inferred from direct assay. Source: MGI intercellular canaliculus Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI tight junction Inferred from direct assay. Source: MGI
Molecular function	protein domain specific binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Actn4	P57780	3	EBI-79508,EBI-445071
CGN	Q9P2M7	1	EBI-79508,EBI-79537	From a different organism.
cgn	Q9PTD7	1	EBI-79508,EBI-79525	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1745

1745

Tight junction protein ZO-1

PRO_0000094541

Regions

Domain

23 – 110

PDZ 1

Domain

186 – 264

PDZ 2

Domain

421 – 502

PDZ 3

Domain

516 – 584

SH3

Domain

610 – 791

182

Guanylate kinase-like

Domain

1629 – 1721

ZU5

Compositional bias

1242 – 1247

Poly-Pro

Compositional bias

1424 – 1430

Poly-Pro

Amino acid modifications

Modified residue

125

Phosphoserine By similarity

Modified residue

131

Phosphoserine By similarity

Modified residue

132

Phosphotyrosine By similarity

Modified residue

166

Phosphoserine By similarity

Modified residue

168

Phosphoserine By similarity

Modified residue

175

Phosphoserine

Modified residue

275

Phosphoserine By similarity

Modified residue

277

Phosphoserine By similarity

Modified residue

280

Phosphoserine By similarity

Modified residue

284

Phosphoserine

Modified residue

297

Phosphoserine

Modified residue

300

Phosphoserine

Modified residue

329

Phosphoserine By similarity

Modified residue

337

Phosphoserine By similarity

Modified residue

402

Phosphoserine By similarity

Modified residue

617

Phosphoserine

Modified residue

868

Phosphothreonine By similarity

Modified residue

912

Phosphoserine

Modified residue

927

Phosphoserine By similarity

Modified residue

1139

Phosphotyrosine

Modified residue

1353

Phosphotyrosine

Modified residue

1365

Phosphoserine By similarity

Modified residue

1542

Phosphoserine By similarity

Modified residue

1614

Phosphoserine By similarity

Modified residue

1616

Phosphoserine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P39447-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: C3DA2C0A9F411F66
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FASTA

1,745

194,711

        10         20         30         40         50         60 
MSARAAAAKS TAMEETAIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG ETSIVISDVL 

        70         80         90        100        110        120 
KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK NAKITIRRKK KVQIPVSHPD 

       130        140        150        160        170        180 
PEPVSDNEDD SYDEEVHDPR AGRGALANRR SEKSWARDRS ASRERSLSPR SDRRSVASSQ 

       190        200        210        220        230        240 
PAKPTKVTLV KSRKNEEYGL RPASHIFVKE ISQDSLAARD GDIQEGDVVL KINGTVTENM 

       250        260        270        280        290        300 
SLTDAKTLIE RSKGKLKMVV QRDERATLLN VPDLSDSIHS ANASERDDIS EIQSLASDHS 

       310        320        330        340        350        360 
GRSHDRPPRR SQSRSPDQRS EPSDHSTQSP QQPSNGSLRS REEERMSKPG AISTPVKHVD 

       370        380        390        400        410        420 
DHPPKAVEEV TVEKNEKQTP TLPEPKPVYA QVGQPDVDLP VSPSDGALPN SAHEDGILRP 

       430        440        450        460        470        480 
SMKLVKFRKG DSVGLRLAGG NDVGIFVAGV LEDSPAAKEG LEEGDQILRV NNVDFTNIIR 

       490        500        510        520        530        540 
EEAVLFLLDL PKGEEVTILA QKKKDVYRRI VESDVGDSFY IRTHFEYEKE SPYGLSFNKG 

       550        560        570        580        590        600 
EVFRVVDTLY NGKLGSWLAI RIGKNHKEVE RGIIPNKNRA EQLASVQYTL PKTAGGDRAD 

       610        620        630        640        650        660 
FWRFRGLRSS KRNLRKSRED LSAQPVQTKF PAYERVVLRE AGFLRPVTIF GPIADVAREK 

       670        680        690        700        710        720 
LAREEPDIYQ IAKSELRDAG TDHRSSGIIR LHTIKQIIDQ DKHALLDVTP NAVDRLNYAQ 

       730        740        750        760        770        780 
WYPIVVFLNP DSKQGVKTMR MRLCPESRKS ARKLYERSHK LRKNNHHLFT TTINLNSMND 

       790        800        810        820        830        840 
GWYGALKEAI QQQQNQLVWV SEGKADGATS DDLDLHDDRL SYLSAPGSEY SMYSTDSRHT 

       850        860        870        880        890        900 
SDYEDTDTEG GAYTDQELDE TLNDEVGTPP ESAITRSSEP VREDSSGMHH ENQTYPPYSP 

       910        920        930        940        950        960 
QAQPQAIHRI DSPGLKPASQ QKAEASSPVP YLSPETTPAS SASAVNHNVS VTNVSLEEPA 

       970        980        990       1000       1010       1020 
PAPPTSHASQ PGCLGAPSAE AAHVGLRGEG PPLPPHADPA KVYRKEPYSE EMMRQNHILK 

      1030       1040       1050       1060       1070       1080 
QPALGHPGQR PDKEPNLAYE PQLPYIEKQA SRDLEQPSYR YEVSSYTDQF SRNYDHRLRF 

      1090       1100       1110       1120       1130       1140 
EDRIPTYEDQ WSYYDDKQPY QPRPFENQHP RDLDSRQHPE EASERGYFQR FEEPAPLSYD 

      1150       1160       1170       1180       1190       1200 
SRTRYEQLPR TSTLRHEEQP APAYEVHNRY RPEAQPYSST GPKSSEPKQY FDQYPRSYEQ 

      1210       1220       1230       1240       1250       1260 
VPPPGFTSKT GHYEPLHGAA VVPPLIPSSQ QKPEVLRSAT KPQPPPPTLT EEEEDPAMKP 

      1270       1280       1290       1300       1310       1320 
QSVLTRVKMF ENKRSASLEN KKDVNDTASF KPPEVASKPP GASLAGPKPV PQSQFSEHDK 

      1330       1340       1350       1360       1370       1380 
TLYRLPEPQK PQVKPPEDIV RSNHYDPEED EEYYRKQLSY FDRRSFESKP SAHLPAGHHS 

      1390       1400       1410       1420       1430       1440 
EPAKPVHSQS QPNFPSYSSK GKPETDAVDR SFSEKRYDPA QATPPPPPLP SQYSQPAPPL 

      1450       1460       1470       1480       1490       1500 
SSSSLHIHSK GAQGEGNSVS LDFQNSYMSK PDPPPSQSKP ATFRPPTRED PPQTFYPQKS 

      1510       1520       1530       1540       1550       1560 
FPDKAPVNGA EQTQKTITPV YNRFTPKPYT SSARPFERKF ESPKFNHNLL PSETVHKPEL 

      1570       1580       1590       1600       1610       1620 
SSKTPTSPKT LMKAHSSTQP PEFGSGVETF SVHTDKPKYQ MNNISTMPKA VPVSPSAVEE 

      1630       1640       1650       1660       1670       1680 
DEDEDGHTVV ATARGIFNSN GGVLSSIETG VSIIIPQGAI PEGIEQEIYF KVCRDNSILP 

      1690       1700       1710       1720       1730       1740 
PLDKEKGETL LSPLVMCGPH GLKFLKPVEL RLPHCDPKTW QNKCLPGDPN YLVGANCVSV 


LIDHF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy." Itoh M., Nagafuchi A., Yonemura S., Yasuda-Kitani T., Tsukita S., Tsukita S. J. Cell Biol. 121:491-502(1993) [PubMed: 8486731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129.
[2]	"NEPH1 defines a novel family of podocin interacting proteins." Sellin L., Huber T.B., Gerke P., Quack I., Pavenstaedt H., Walz G. FASEB J. 17:115-117(2003) [PubMed: 12424224] [Abstract] Cited for: INTERACTION WITH KIRREL1. Strain: Swiss Webster. Tissue: Brain.
[3]	"Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain." Li X., Ionescu A.V., Lynn B.D., Lu S., Kamasawa N., Morita M., Davidson K.G.V., Yasumura T., Rash J.E., Nagy J.I. Neuroscience 126:611-630(2004) [PubMed: 15183511] [Abstract] Cited for: INTERACTION WITH GJA12, DOMAIN.
[4]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1139 AND TYR-1353, MASS SPECTROMETRY. Tissue: Mast cell.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-175; SER-284; SER-297; SER-300 AND SER-617, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-912, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	D14340 mRNA. Translation: BAA03274.1.
PIR	A46431.
RefSeq	NP_033412.1.
UniGene	Mm.4342
3D structure databases
HSSP	HSSP built from PDB template 1JXO based on UniProtKB P31016.
SMR	P39447. Positions 18-110.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39447.
PTM databases
PhosphoSite	P39447.
Genome annotation databases
Ensembl	ENSMUSG00000030516. Mus musculus. [Contig view]
GeneID	21872.
KEGG	mmu:21872.
Organism-specific databases
MGI	MGI:98759. Tjp1.
Phylogenomic databases
HOGENOM	P39447.
HOVERGEN	P39447.
Gene expression databases
ArrayExpress	P39447.
CleanEx	MM_TJP1.
GermOnline	ENSMUSG00000030516. Mus musculus.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylt/Ca. IPR001478. PDZ. IPR001452. SH3. IPR011511. SH3_2. IPR005417. ZonOcculdens. IPR005418. ZonOcculS1. IPR000906. ZU5. [Graphical view]
PANTHER	PTHR13865:SF9. ZonOcculS1. 1 hit.
Pfam	PF00625. Guanylate_kin. 1 hit. PF00595. PDZ. 3 hits. PF07653. SH3_2. 1 hit. PF00791. ZU5. 1 hit. [Graphical view]
PRINTS	PR01597. ZONOCCLUDNS. PR01598. ZONOCCLUDNS1.
SMART	SM00072. GuKc. 1 hit. SM00228. PDZ. 3 hits. SM00326. SH3. 1 hit. SM00218. ZU5. 1 hit. [Graphical view]
PROSITE	PS00856. GUANYLATE_KINASE_1. False negative. PS50052. GUANYLATE_KINASE_2. 1 hit. PS50106. PDZ. 3 hits. PS50002. SH3. 1 hit. PS51145. ZU5. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301376.
SOURCE	Search...

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Entry information

Entry name

ZO1_MOUSE

Accession

Primary (citable) accession number: P39447

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents