Reviewed, UniProtKB/Swiss-Prot P39442 (HCY_NATPH)

Names and origin

Protein names

Recommended name:
    Halocyanin

Gene names

Name:

hcy

Organism

Natronomonas pharaonis (Natronobacterium pharaonis)

Taxonomic identifier

2257 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Natronomonas

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Electron donor. Binds one copper ion.
Subcellular location	Cell membrane; Lipid-anchor.
Sequence similarities	Contains 1 plastocyanin-like domain.
Biophysicochemical properties	Redox potential: E0 is +183 mV.
Mass spectrometry	Molecular mass is 15456±1.5 Da from positions 25 - 163. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Copper Metal-binding
PTM	Acetylation Lipoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 163

139

Halocyanin

PRO_0000002871

Regions

Domain

48 – 163

116

Plastocyanin-like

Sites

Metal binding

110

1

Copper By similarity

Metal binding

148

1

Copper By similarity

Metal binding

151

1

Copper By similarity

Metal binding

156

1

Copper By similarity

Amino acid modifications

Modified residue

25

1

N-acetylcysteine

Lipidation

25

1

S-archaeol cysteine

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

.

.

.

163

Helix Strand Turn

Details...

Beta strand

72 – 76

5

Beta strand

78 – 82

5

Beta strand

85 – 92

8

Beta strand

98 – 103

6

Beta strand

105 – 108

4

Turn

116 – 118

3

Beta strand

119 – 122

4

Beta strand

133 – 137

5

Beta strand

144 – 147

4

Helix

149 – 152

4

Turn

153 – 155

3

Beta strand

157 – 161

5

Sequences

	Sequence		Length	Mass (Da)
	P39442-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DB180417F8B0BEE6	FASTA	163	17,222
	10 20 30 40 50 60 MKDISRRRFV LGTGATVAAA TLAGCNGNGN GNGNGNGNGE PDTPEGRADQ FLTDNDALMY 70 80 90 100 110 120 DGDITDETGQ DEVVVVTGAG NNGFAFDPAA IRVDVGTTVT WEWTGDGGAH NVVSEPESDF 130 140 150 160 EFESDRVDEE GFTFEQTFDD EGVALYVCTP HRAQGMYGAV IVE

References

[1]	"The primary structure of halocyanin, an archaeal blue copper protein, predicts a lipid anchor for membrane fixation." Mattar S., Scharf B., Kent S.B.H., Rodewald K., Oesterhelt D., Engelhard M. J. Biol. Chem. 269:14939-14945(1994) [PubMed: 8195126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY. Strain: SP-1 / 28.
[2]	"Halocyanin, an archaebacterial blue copper protein (type I) from Natronobacterium pharaonis." Scharf B., Engelhard M. Biochemistry 32:12894-12900(1993) [PubMed: 8251512] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 144-163.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z30236 Genomic DNA. Translation: CAA82942.1.

PIR

A53792.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N3D	model	-	A	69-163	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR000923. BlueCu_1.
IPR008972. Cupredoxin.
IPR017533. Halocyanin.
IPR006311. TAT_signal.
IPR017909. Twin_arg_translocation_Tat.
[Graphical view]

Gene3D

G3DSA:2.60.40.420. Cupredoxin. 1 hit.

Pfam

PF00127. Copper-bind. 1 hit.
[Graphical view]

PROSITE

PS00196. COPPER_BLUE. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HCY_NATPH

Accession

Primary (citable) accession number: P39442
Secondary accession number(s): Q9UWL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 15, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)