Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-tartrate/succinate antiporter

Gene

ttdT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the uptake of tartrate in exchange for intracellular succinate. Essential for anaerobic L-tartrate fermentation.1 Publication

Kineticsi

  1. KM=700 µM for tartrate1 Publication
  2. KM=400 µM for succinate1 Publication
  1. Vmax=110 µmol/min/g enzyme for tartrate import1 Publication
  2. Vmax=16 µmol/min/g enzyme for succinate export1 Publication

GO - Molecular functioni

  • antiporter activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGJE-MONOMER.
ECOL316407:JW3035-MONOMER.
MetaCyc:YGJE-MONOMER.

Protein family/group databases

TCDBi2.A.47.3.3. the divalent anion:na(+) symporter (dass) family.

Names & Taxonomyi

Protein namesi
Recommended name:
L-tartrate/succinate antiporter
Alternative name(s):
Tartrate carrier
Tartrate transporter
Gene namesi
Name:ttdT
Synonyms:ygjC, ygjE
Ordered Locus Names:b3063, JW3035
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12393. ttdT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9PeriplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 32CytoplasmicSequence analysis2
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 92CytoplasmicSequence analysisAdd BLAST18
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 136PeriplasmicSequence analysisAdd BLAST23
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Topological domaini158 – 188CytoplasmicSequence analysisAdd BLAST31
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 235PeriplasmicSequence analysisAdd BLAST26
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 291CytoplasmicSequence analysisAdd BLAST35
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 339CytoplasmicSequence analysis6
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Topological domaini361 – 369PeriplasmicSequence analysis9
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Topological domaini391 – 392CytoplasmicSequence analysis2
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 417PeriplasmicSequence analysis4
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 464CytoplasmicSequence analysisAdd BLAST26
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21
Topological domaini486 – 487PeriplasmicSequence analysis2

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001725251 – 487L-tartrate/succinate antiporterAdd BLAST487

Proteomic databases

PaxDbiP39414.
PRIDEiP39414.

Expressioni

Inductioni

Induced by tartrate, via TtdR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262386. 10 interactors.
STRINGi511145.b3063.

Structurei

3D structure databases

ProteinModelPortaliP39414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QPK. Bacteria.
COG0471. LUCA.
HOGENOMiHOG000117911.
InParanoidiP39414.
KOiK11106.
OMAiNAWLYFC.
PhylomeDBiP39414.

Family and domain databases

InterProiIPR030676. CitT-rel.
IPR001898. Na/sul_symport.
[Graphical view]
PANTHERiPTHR10283:SF13. PTHR10283:SF13. 2 hits.
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PIRSFiPIRSF002457. DASS. 1 hit.
TIGRFAMsiTIGR00785. dass. 1 hit.

Sequencei

Sequence statusi: Complete.

P39414-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSTEWWRY LAPLAVIAII ALLPVPAGLE NHTWLYFAVF TGVIVGLILE
60 70 80 90 100
PVPGAVVAMV GISIIAILSP WLLFSPEQLA QPGFKFTAKS LSWAVSGFSN
110 120 130 140 150
SVIWLIFAAF MFGTGYEKTG LGRRIALILV KKMGHRTLFL GYAVMFSELI
160 170 180 190 200
LAPVTPSNSA RGAGIIYPII RNLPPLYQSQ PNDSSSRSIG SYIMWMGIVA
210 220 230 240 250
DCVTSAIFLT AMAPNLLLIG LMKSASHATL SWGDWFLGML PLSILLVLLV
260 270 280 290 300
PWLAYVLYPP VLKSGDQVPR WAETELQAMG PLCSREKRML GLMVGALVLW
310 320 330 340 350
IFGGDYIDAA MVGYSVVALM LLLRIISWDD IVSNKAAWNV FFWLASLITL
360 370 380 390 400
ATGLNNTGFI SWFGKLLAGS LSGYSPTMVM VALIVVFYLL RYFFASATAY
410 420 430 440 450
TSALAPMMIA AALAMPEIPL PVFCLMVGAA IGLGSILTPY ATGPSPIYYG
460 470 480
SGYLPTADYW RLGAIFGLIF LVLLVITGLL WMPVVLL
Length:487
Mass (Da):52,906
Last modified:November 1, 1997 - v2
Checksum:i4D6E8439BEE281D2
GO

Sequence cautioni

The sequence M16194 differs from that shown. Reason: Frameshift at positions 35, 51, 132, 245, 268 and 443.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti404L → P (PubMed:3297921).Curated1
Sequence conflicti457A → T (PubMed:3297921).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16194 Genomic DNA. No translation available.
U28379 Genomic DNA. Translation: AAA89143.1.
U00096 Genomic DNA. Translation: AAC76099.1.
AP009048 Genomic DNA. Translation: BAE77114.1.
PIRiE65094. QQECRS.
RefSeqiNP_417535.1. NC_000913.3.
WP_000804973.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76099; AAC76099; b3063.
BAE77114; BAE77114; BAE77114.
GeneIDi947576.
KEGGiecj:JW3035.
eco:b3063.
PATRICi32121540. VBIEscCol129921_3157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16194 Genomic DNA. No translation available.
U28379 Genomic DNA. Translation: AAA89143.1.
U00096 Genomic DNA. Translation: AAC76099.1.
AP009048 Genomic DNA. Translation: BAE77114.1.
PIRiE65094. QQECRS.
RefSeqiNP_417535.1. NC_000913.3.
WP_000804973.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262386. 10 interactors.
STRINGi511145.b3063.

Protein family/group databases

TCDBi2.A.47.3.3. the divalent anion:na(+) symporter (dass) family.

Proteomic databases

PaxDbiP39414.
PRIDEiP39414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76099; AAC76099; b3063.
BAE77114; BAE77114; BAE77114.
GeneIDi947576.
KEGGiecj:JW3035.
eco:b3063.
PATRICi32121540. VBIEscCol129921_3157.

Organism-specific databases

EchoBASEiEB2294.
EcoGeneiEG12393. ttdT.

Phylogenomic databases

eggNOGiENOG4107QPK. Bacteria.
COG0471. LUCA.
HOGENOMiHOG000117911.
InParanoidiP39414.
KOiK11106.
OMAiNAWLYFC.
PhylomeDBiP39414.

Enzyme and pathway databases

BioCyciEcoCyc:YGJE-MONOMER.
ECOL316407:JW3035-MONOMER.
MetaCyc:YGJE-MONOMER.

Miscellaneous databases

PROiP39414.

Family and domain databases

InterProiIPR030676. CitT-rel.
IPR001898. Na/sul_symport.
[Graphical view]
PANTHERiPTHR10283:SF13. PTHR10283:SF13. 2 hits.
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PIRSFiPIRSF002457. DASS. 1 hit.
TIGRFAMsiTIGR00785. dass. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTTDT_ECOLI
AccessioniPrimary (citable) accession number: P39414
Secondary accession number(s): Q2M9E2, Q46870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.