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Protein

Putative glycyl-radical enzyme activating enzyme YjjW

Gene

yjjW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

[4Fe-4S] clusterBy similarityNote: May bind up to 3 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi35Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi38Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi47Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi50Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi53Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi57Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi76Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi79Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi86Iron-sulfur 2 (4Fe-4S)By similarity1
Binding sitei121S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei246S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7953-MONOMER.
ECOL316407:JW4342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glycyl-radical enzyme activating enzyme YjjWCurated (EC:1.97.1.-Curated)
Short name:
GRE activating enzyme YjjWCurated
Gene namesi
Name:yjjW
Ordered Locus Names:b4379, JW4342
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12599. yjjW.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005421 – 287Putative glycyl-radical enzyme activating enzyme YjjWAdd BLAST287

Proteomic databases

PaxDbiP39409.
PRIDEiP39409.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dpiAP0AEF42EBI-9132384,EBI-1119284
galEP091472EBI-9132384,EBI-909010
minCP181962EBI-9132384,EBI-554060

Protein-protein interaction databases

BioGridi4263478. 4 interactors.
IntActiP39409. 25 interactors.
STRINGi511145.b4379.

Structurei

3D structure databases

ProteinModelPortaliP39409.
SMRiP39409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 674Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini67 – 964Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 39S-adenosyl-L-methionine bindingBy similarity3
Regioni174 – 176S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG41076CZ. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011457.
InParanoidiP39409.
OMAiCHEVNIH.
PhylomeDBiP39409.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012839. Organic_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
IPR023912. YjjW_bact.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMsiTIGR04041. activase_YjjW. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSRCALVSK IIPFSCVDGP GSRLALFLQG CNLRCKNCHN PWTMGRCNDC
60 70 80 90 100
GECVPQCPHQ ALQIVDGKVV WNAVVCEQCD TCLKRCPQHA TPMAQSMSVD
110 120 130 140 150
EVLSHVRKAV LFIEGITVSG GEATTQLPFV VALFTAIKND PQLRHLTCLV
160 170 180 190 200
DSNGMLSETG WEKLLPVCDG AMLDLKAWGS ECHQQLTGRD NQQIKRSIYL
210 220 230 240 250
LAERGKLAEL RLLVIPGQVD YLQHIEELAA FIKGLGDVPV RLNAFHAHGV
260 270 280
YGEAQSWASA TPEDVEPLAD ALKVRGVSRL IFPALYL
Length:287
Mass (Da):31,491
Last modified:February 1, 1995 - v1
Checksum:iE08BB429519E54B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97275.1.
U00096 Genomic DNA. Translation: AAC77332.1.
AP009048 Genomic DNA. Translation: BAE78368.1.
PIRiS56603.
RefSeqiNP_418796.1. NC_000913.3.
WP_001088415.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77332; AAC77332; b4379.
BAE78368; BAE78368; BAE78368.
GeneIDi948905.
KEGGiecj:JW4342.
eco:b4379.
PATRICi32124370. VBIEscCol129921_4525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97275.1.
U00096 Genomic DNA. Translation: AAC77332.1.
AP009048 Genomic DNA. Translation: BAE78368.1.
PIRiS56603.
RefSeqiNP_418796.1. NC_000913.3.
WP_001088415.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39409.
SMRiP39409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263478. 4 interactors.
IntActiP39409. 25 interactors.
STRINGi511145.b4379.

Proteomic databases

PaxDbiP39409.
PRIDEiP39409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77332; AAC77332; b4379.
BAE78368; BAE78368; BAE78368.
GeneIDi948905.
KEGGiecj:JW4342.
eco:b4379.
PATRICi32124370. VBIEscCol129921_4525.

Organism-specific databases

EchoBASEiEB2484.
EcoGeneiEG12599. yjjW.

Phylogenomic databases

eggNOGiENOG41076CZ. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011457.
InParanoidiP39409.
OMAiCHEVNIH.
PhylomeDBiP39409.

Enzyme and pathway databases

BioCyciEcoCyc:G7953-MONOMER.
ECOL316407:JW4342-MONOMER.

Miscellaneous databases

PROiP39409.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012839. Organic_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
IPR023912. YjjW_bact.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMsiTIGR04041. activase_YjjW. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYJJW_ECOLI
AccessioniPrimary (citable) accession number: P39409
Secondary accession number(s): Q2M5T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.