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Protein

Ribosomal RNA small subunit methyltransferase C

Gene

rsmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanine in position 1207 of 16S rRNA in the 30S particle.

Catalytic activityi

S-adenosyl-L-methionine + guanine(1207) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1207) in 16S rRNA.

Cofactori

GO - Molecular functioni

  • 16S rRNA (guanine(1207)-N(2))-methyltransferase activity Source: EcoCyc
  • nucleic acid binding Source: InterPro
  • rRNA (guanine-N2-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
  • rRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

Magnesium, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7950-MONOMER.
ECOL316407:JW4333-MONOMER.
MetaCyc:G7950-MONOMER.
BRENDAi2.1.1.172. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase C (EC:2.1.1.172)
Alternative name(s):
16S rRNA m2G1207 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RsmC
Gene namesi
Name:rsmC
Synonyms:yjjT
Ordered Locus Names:b4371, JW4333
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12596. rsmC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86K → S: Reduces activity by 84%; when associated with S-88. 1 Publication1
Mutagenesisi88K → S: Reduces activity by 84%; when associated with S-86. 1 Publication1
Mutagenesisi202D → A: Abolishes affinity for S-adenosyl-L-methionine. Loss of activity. 1 Publication1
Mutagenesisi227D → A: Strongly reduces affinity for S-adenosyl-L-methionine. Reduces activity by 87%. 1 Publication1
Mutagenesisi268N → A: Reduces affinity for S-adenosyl-L-methionine. Reduces activity by 80%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000974902 – 343Ribosomal RNA small subunit methyltransferase CAdd BLAST342

Proteomic databases

EPDiP39406.
PaxDbiP39406.
PRIDEiP39406.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262778. 9 interactors.
DIPiDIP-10804N.
IntActiP39406. 14 interactors.
STRINGi511145.b4371.

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 12Combined sources7
Helixi15 – 18Combined sources4
Beta strandi22 – 27Combined sources6
Helixi33 – 36Combined sources4
Beta strandi40 – 48Combined sources9
Helixi49 – 59Combined sources11
Helixi60 – 62Combined sources3
Beta strandi63 – 65Combined sources3
Helixi71 – 74Combined sources4
Beta strandi78 – 83Combined sources6
Helixi88 – 99Combined sources12
Beta strandi107 – 113Combined sources7
Helixi114 – 116Combined sources3
Helixi118 – 120Combined sources3
Helixi121 – 125Combined sources5
Turni126 – 128Combined sources3
Beta strandi132 – 134Combined sources3
Beta strandi140 – 146Combined sources7
Helixi155 – 158Combined sources4
Beta strandi160 – 164Combined sources5
Beta strandi167 – 171Combined sources5
Beta strandi178 – 180Combined sources3
Helixi183 – 191Combined sources9
Helixi209 – 217Combined sources9
Beta strandi224 – 229Combined sources6
Helixi230 – 242Combined sources13
Beta strandi248 – 251Combined sources4
Turni254 – 257Combined sources4
Beta strandi262 – 267Combined sources6
Beta strandi273 – 275Combined sources3
Helixi276 – 289Combined sources14
Helixi290 – 292Combined sources3
Beta strandi293 – 304Combined sources12
Helixi309 – 317Combined sources9
Beta strandi321 – 325Combined sources5
Beta strandi327 – 335Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PJDX-ray2.10A1-343[»]
ProteinModelPortaliP39406.
SMRiP39406.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39406.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D8J. Bacteria.
COG2813. LUCA.
HOGENOMiHOG000218308.
InParanoidiP39406.
KOiK00564.
OMAiRHCQLWQ.
PhylomeDBiP39406.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01862. 16SrRNA_methyltr_C. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR013675. Mtase_sm_N.
IPR023543. rRNA_ssu_MeTfrase_C.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
PF08468. MTS_N. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFTPASEV LLRHSDDFEQ SRILFAGDLQ DDLPARLDTA ASRAHTQQFH
60 70 80 90 100
HWQVLSRQMG DNARFSLVAT ADDVADCDTL IYYWPKNKPE AQFQLMNLLS
110 120 130 140 150
LLPVGTDIFV VGENRSGVRS AEQMLADYAP LNKVDSARRC GLYFGRLEKQ
160 170 180 190 200
PVFDAEKFWG EYSVDGLTVK TLPGVFSRDG LDVGSQLLLS TLTPHTKGKV
210 220 230 240 250
LDVGCGAGVL SVAFARHSPK IRLTLCDVSA PAVEASRATL AANGVEGEVF
260 270 280 290 300
ASNVFSEVKG RFDMIISNPP FHDGMQTSLD AAQTLIRGAV RHLNSGGELR
310 320 330 340
IVANAFLPYP DVLDETFGFH EVIAQTGRFK VYRAIMTRQA KKG
Length:343
Mass (Da):37,625
Last modified:January 23, 2007 - v3
Checksum:i81E190CB86E4BAC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97267.1.
U00096 Genomic DNA. Translation: AAC77324.1.
AP009048 Genomic DNA. Translation: BAE78359.1.
PIRiS56595.
RefSeqiNP_418788.1. NC_000913.3.
WP_001272330.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77324; AAC77324; b4371.
BAE78359; BAE78359; BAE78359.
GeneIDi948892.
KEGGiecj:JW4333.
eco:b4371.
PATRICi32124352. VBIEscCol129921_4516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97267.1.
U00096 Genomic DNA. Translation: AAC77324.1.
AP009048 Genomic DNA. Translation: BAE78359.1.
PIRiS56595.
RefSeqiNP_418788.1. NC_000913.3.
WP_001272330.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PJDX-ray2.10A1-343[»]
ProteinModelPortaliP39406.
SMRiP39406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262778. 9 interactors.
DIPiDIP-10804N.
IntActiP39406. 14 interactors.
STRINGi511145.b4371.

Proteomic databases

EPDiP39406.
PaxDbiP39406.
PRIDEiP39406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77324; AAC77324; b4371.
BAE78359; BAE78359; BAE78359.
GeneIDi948892.
KEGGiecj:JW4333.
eco:b4371.
PATRICi32124352. VBIEscCol129921_4516.

Organism-specific databases

EchoBASEiEB2481.
EcoGeneiEG12596. rsmC.

Phylogenomic databases

eggNOGiENOG4105D8J. Bacteria.
COG2813. LUCA.
HOGENOMiHOG000218308.
InParanoidiP39406.
KOiK00564.
OMAiRHCQLWQ.
PhylomeDBiP39406.

Enzyme and pathway databases

BioCyciEcoCyc:G7950-MONOMER.
ECOL316407:JW4333-MONOMER.
MetaCyc:G7950-MONOMER.
BRENDAi2.1.1.172. 2026.

Miscellaneous databases

EvolutionaryTraceiP39406.
PROiP39406.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01862. 16SrRNA_methyltr_C. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR013675. Mtase_sm_N.
IPR023543. rRNA_ssu_MeTfrase_C.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
PF08468. MTS_N. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMC_ECOLI
AccessioniPrimary (citable) accession number: P39406
Secondary accession number(s): Q2M5U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.