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Protein

Transcriptional activator protein BglJ

Gene

bglJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A crytic transcriptional activator. When its expression is induced it relieves H-NS repression of the bgl operon. Acts independently of transcription factor LeuO.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi170 – 18920H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7948-MONOMER.
ECOL316407:JW5955-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein BglJ
Gene namesi
Name:bglJ
Synonyms:yjjR
Ordered Locus Names:b4366, JW5955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12594. bglJ.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Transcriptional activator protein BglJPRO_0000184140Add
BLAST

Proteomic databases

PaxDbiP39404.

Expressioni

Inductioni

Repressed by H-NS, activated by LeuO. Member of the yjjQ-bglJ operon.2 Publications

Interactioni

Subunit structurei

Forms a complex with RcsB; genetically both BglJ and RcsB are required to relieve bgl operon repression by H-NS and by StpA.

Binary interactionsi

WithEntry#Exp.IntActNotes
rcsBP0DMC72EBI-551429,EBI-369670

Protein-protein interaction databases

BioGridi4262783. 126 interactions.
DIPiDIP-9217N.
IntActiP39404. 2 interactions.
MINTiMINT-1268192.
STRINGi511145.b4366.

Structurei

3D structure databases

ProteinModelPortaliP39404.
SMRiP39404. Positions 151-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini146 – 21166HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108T94. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000127596.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHSRIKKRN VALIEKCVMS SIGIESLFRK FAGNPYKLHT YTSQESFQDA
60 70 80 90 100
MSRISFAAVI FSFSAMRSER REGLSCLTEL AIKFPRTRRL VIADDDIEAR
110 120 130 140 150
LIGSLSPSPL DGVLSKASTL EIFHQELFLS LNGVRQATDR LNNQWYINQS
160 170 180 190 200
RTLSPTEREI LRFMSRGYSM TQIAEQLKRN IKTIRAHKFN VMSKLGVSSD
210 220
AGLLEAADIL LCMRHCETSN VLHPY
Length:225
Mass (Da):25,622
Last modified:September 21, 2011 - v3
Checksum:i5EED6E444409A010
GO

Sequence cautioni

The sequence BAE78356.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35834 Genomic DNA. Translation: AAB49332.1.
U14003 Genomic DNA. Translation: AAA97265.1.
U00096 Genomic DNA. Translation: AAC77322.4.
AP009048 Genomic DNA. Translation: BAE78356.1. Different initiation.
RefSeqiNP_418786.4. NC_000913.3.
WP_001301727.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77322; AAC77322; b4366.
BAE78356; BAE78356; BAE78356.
GeneIDi948889.
KEGGiecj:JW5955.
eco:b4366.
PATRICi32124340. VBIEscCol129921_4513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35834 Genomic DNA. Translation: AAB49332.1.
U14003 Genomic DNA. Translation: AAA97265.1.
U00096 Genomic DNA. Translation: AAC77322.4.
AP009048 Genomic DNA. Translation: BAE78356.1. Different initiation.
RefSeqiNP_418786.4. NC_000913.3.
WP_001301727.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39404.
SMRiP39404. Positions 151-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262783. 126 interactions.
DIPiDIP-9217N.
IntActiP39404. 2 interactions.
MINTiMINT-1268192.
STRINGi511145.b4366.

Proteomic databases

PaxDbiP39404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77322; AAC77322; b4366.
BAE78356; BAE78356; BAE78356.
GeneIDi948889.
KEGGiecj:JW5955.
eco:b4366.
PATRICi32124340. VBIEscCol129921_4513.

Organism-specific databases

EchoBASEiEB2479.
EcoGeneiEG12594. bglJ.

Phylogenomic databases

eggNOGiENOG4108T94. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000127596.

Enzyme and pathway databases

BioCyciEcoCyc:G7948-MONOMER.
ECOL316407:JW5955-MONOMER.

Miscellaneous databases

PROiP39404.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12."
    Giel M., Desnoyer M., Lopilato J.
    Genetics 143:627-635(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN BGL OPERON EXPRESSION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO."
    Stratmann T., Madhusudan S., Schnetz K.
    J. Bacteriol. 190:926-935(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: OPERON STRUCTURE, INDUCTION.
    Strain: CSH50.
  6. "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux."
    Shimada T., Yamamoto K., Ishihama A.
    J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: OPERON STRUCTURE, INDUCTION.
    Strain: K12 / BW25113.
  7. "BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS."
    Venkatesh G.R., Kembou Koungni F.C., Paukner A., Stratmann T., Blissenbach B., Schnetz K.
    J. Bacteriol. 192:6456-6464(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN START SITE, INTERACTION WITH RCSB.
    Strain: CSH50.

Entry informationi

Entry nameiBGLJ_ECOLI
AccessioniPrimary (citable) accession number: P39404
Secondary accession number(s): Q2M5V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 21, 2011
Last modified: April 13, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.