P39404 (BGLJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator protein BglJ

Gene names

Name:	bglJ
Synonyms:	yjjR
Ordered Locus Names:	b4366, JW5955

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A crytic transcriptional activator. When its expression is induced it relieves H-NS repression of the bgl operon. Acts independently of transcription factor LeuO. Ref.1
Subunit structure	Forms a complex with RcsB; genetically both BglJ and RcsB are required to relieve bgl operon repression by H-NS and by StpA.
Induction	Repressed by H-NS, activated by LeuO. Member of the yjjQ-bglJ operon. Ref.5
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain.
Sequence caution	The sequence AAC77322.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAE78356.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: EcoCyc two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Transcriptional activator protein BglJ		PRO_0000184140
Regions
	Domain	146 – 211	66	HTH luxR-type
	DNA binding	170 – 189	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39404 [UniParc]. Last modified September 21, 2011. Version 3. Checksum: 5EED6E444409A010	FASTA	225	25,622
	10 20 30 40 50 60 MEHSRIKKRN VALIEKCVMS SIGIESLFRK FAGNPYKLHT YTSQESFQDA MSRISFAAVI 70 80 90 100 110 120 FSFSAMRSER REGLSCLTEL AIKFPRTRRL VIADDDIEAR LIGSLSPSPL DGVLSKASTL 130 140 150 160 170 180 EIFHQELFLS LNGVRQATDR LNNQWYINQS RTLSPTEREI LRFMSRGYSM TQIAEQLKRN 190 200 210 220 IKTIRAHKFN VMSKLGVSSD AGLLEAADIL LCMRHCETSN VLHPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12." Giel M., Desnoyer M., Lopilato J. Genetics 143:627-635(1996) [PubMed: 8725214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN BGL OPERON EXPRESSION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO." Stratmann T., Madhusudan S., Schnetz K. J. Bacteriol. 190:926-935(2008) [PubMed: 18055596] [Abstract] Cited for: OPERON STRUCTURE, INDUCTION. Strain: CSH50.
[6]	"BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS." Venkatesh G.R., Kembou Koungni F.C., Paukner A., Stratmann T., Blissenbach B., Schnetz K. J. Bacteriol. 192:6456-6464(2010) [PubMed: 20952573] [Abstract] Cited for: PROTEIN START SITE, INTERACTION WITH RCSB. Strain: CSH50.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35834 Genomic DNA. Translation: AAB49332.1. U14003 Genomic DNA. Translation: AAA97265.1. U00096 Genomic DNA. Translation: AAC77322.3. Different initiation. AP009048 Genomic DNA. Translation: BAE78356.1. Different initiation.
RefSeq	NP_418786.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P39404.
SMR	P39404. Positions 150-210.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9217N.
IntAct	P39404. 1 interaction.
MINT	MINT-1268192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002557; EBESCP00000002557; EBESCG00000002087. EBESCT00000014946; EBESCP00000014237; EBESCG00000014006.
GeneID	948889.
GenomeReviews	Gene locus JW5955 in contig AP009048_GR. Gene locus b4366 in contig U00096_GR.
KEGG	ecj:JW5955. eco:b4366.
PATRIC	32124340. VBIEscCol129921_4513.
Organism-specific databases
EchoBASE	EB2479.
EcoGene	EG12594. bglJ.
Phylogenomic databases
eggNOG	COG2197.
GeneTree	EBGT00050000008666.
HOGENOM	HBG416721.
OMA	ANNESEM.
PhylomeDB	P39404.
ProtClustDB	PRK11475.
Enzyme and pathway databases
BioCyc	EcoCyc:G7948-MONOMER.
Gene expression databases
Genevestigator	P39404.
Family and domain databases
InterPro	IPR016032. Sig_transdc_resp-reg_C-effctor. IPR000792. Tscrpt_reg_LuxR_C. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00196. GerE. 1 hit. [Graphical view]
PRINTS	PR00038. HTHLUXR.
SMART	SM00421. HTH_LUXR. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
PROSITE	PS00622. HTH_LUXR_1. False negative. PS50043. HTH_LUXR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLJ_ECOLI

Accession

Primary (citable) accession number: P39404
Secondary accession number(s): Q2M5V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program