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Protein

L-galactonate-5-dehydrogenase

Gene

lgoD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of L-galactonate to D-tagaturonate. Required for growth on L-galactonate as the sole carbon source. In vitro, can also use L-gulonate.2 Publications

Catalytic activityi

L-galactonate + NAD+ = D-tagaturonate + NADH.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by EDTA.1 Publication

Kineticsi

kcat is 0.51 sec(-1) with L-galactonate.1 Publication

  1. KM=19.5 mM for L-galactonate1 Publication
  1. Vmax=0.8 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Zinc 1; catalyticBy similarity
Metal bindingi65 – 651Zinc 1; catalyticBy similarity
Metal bindingi92 – 921Zinc 2By similarity
Metal bindingi95 – 951Zinc 2By similarity
Metal bindingi98 – 981Zinc 2By similarity
Metal bindingi106 – 1061Zinc 2By similarity
Metal bindingi146 – 1461Zinc 1; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • L-galactonate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7945-MONOMER.
ECOL316407:JW5793-MONOMER.
RETL1328306-WGS:GSTH-501-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-galactonate-5-dehydrogenase1 Publication (EC:1.1.1.-1 Publication)
Gene namesi
Name:lgoDImported
Synonyms:yjjN
Ordered Locus Names:b4358, JW5793
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12590. lgoD.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on L-galactonate as sole carbon source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340L-galactonate-5-dehydrogenasePRO_0000160895Add
BLAST

Proteomic databases

PaxDbiP39400.

Expressioni

Inductioni

Highly up-regulated during growth on L-galactonate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261389. 9 interactions.
STRINGi511145.b4358.

Structurei

3D structure databases

ProteinModelPortaliP39400.
SMRiP39400. Positions 22-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294690.
InParanoidiP39400.
OrthoDBiEOG6ZWJCC.
PhylomeDBiP39400.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTMNVLICQ QPKELVWKQR EIPIPGDNEA LIKIKSVGIC GTDIHAWGGN
60 70 80 90 100
QPFFSYPRVL GHEICGEIVG LGKNIADLKN GQQVAVIPYV ACQQCPACKS
110 120 130 140 150
GRTNCCEKIS VIGVHQDGGF SEYLSVPVAN ILPADGIDPQ AAALIEPFAI
160 170 180 190 200
SAHAVRRAAI APGEQVLVVG AGPIGLGAAA IAKADGAQVV VADTSPARRE
210 220 230 240 250
HVATRLELPL LDPSAEDFDA QLRAQFGGSL AQKVIDATGN QHAMNNTVNL
260 270 280 290 300
IRHGGTVVFV GLFKGELQFS DPEFHKKETT MMGSRNATPE DFAKVGRLMA
310 320 330 340
EGKITADMML THRYPFATLA ETYERDVINN RELIKGVITF
Length:340
Mass (Da):36,448
Last modified:November 24, 2009 - v3
Checksum:i15FD289EFFD05567
GO

Sequence cautioni

The sequence AAA97256.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97256.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77314.4.
AP009048 Genomic DNA. Translation: BAE78348.1.
PIRiS56585.
RefSeqiNP_418778.4. NC_000913.3.
WP_000106030.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77314; AAC77314; b4358.
BAE78348; BAE78348; BAE78348.
GeneIDi948883.
KEGGiecj:JW5793.
eco:b4358.
PATRICi32124324. VBIEscCol129921_4505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97256.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77314.4.
AP009048 Genomic DNA. Translation: BAE78348.1.
PIRiS56585.
RefSeqiNP_418778.4. NC_000913.3.
WP_000106030.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39400.
SMRiP39400. Positions 22-313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261389. 9 interactions.
STRINGi511145.b4358.

Proteomic databases

PaxDbiP39400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77314; AAC77314; b4358.
BAE78348; BAE78348; BAE78348.
GeneIDi948883.
KEGGiecj:JW5793.
eco:b4358.
PATRICi32124324. VBIEscCol129921_4505.

Organism-specific databases

EchoBASEiEB2475.
EcoGeneiEG12590. lgoD.

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294690.
InParanoidiP39400.
OrthoDBiEOG6ZWJCC.
PhylomeDBiP39400.

Enzyme and pathway databases

BioCyciEcoCyc:G7945-MONOMER.
ECOL316407:JW5793-MONOMER.
RETL1328306-WGS:GSTH-501-MONOMER.

Miscellaneous databases

PROiP39400.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: FUNCTION, ROLE IN L-GALACTONATE UTILIZATION, DISRUPTION PHENOTYPE, INDUCTION.
  5. "The yjjN of E. coli codes for an L-galactonate dehydrogenase and can be used for quantification of L-galactonate and L-gulonate."
    Kuivanen J., Richard P.
    Appl. Biochem. Biotechnol. 173:1829-1835(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiLGOD_ECOLI
AccessioniPrimary (citable) accession number: P39400
Secondary accession number(s): Q2M5V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 24, 2009
Last modified: May 11, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.