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Protein

Toxic protein SymE

Gene

symE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation and recycling of damaged RNA. It is itself a target for degradation by the ATP-dependent protease Lon.

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • mRNA catabolic process Source: EcoCyc
  • SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7940-MONOMER.
ECOL316407:JW4310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxic protein SymE
Alternative name(s):
Putative endoribonuclease SymE (EC:3.1.-.-)
Gene namesi
Name:symE
Synonyms:dinL, sosC, yjiW
Ordered Locus Names:b4347, JW4310
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12584. symE.

Subcellular locationi

  • Cytoplasm Curated

  • Note: Purifies with ribosomes.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001697991 – 113Toxic protein SymEAdd BLAST113

Proteomic databases

PaxDbiP39394.
PRIDEiP39394.

Expressioni

Inductioni

During the SOS response (at protein level when expressed as a fusion protein). In normal growth conditions, it is repressed by LexA. The mRNA stability and translation is controlled by the small regulatory RNA symR, which probably blocks ribosome binding. A type I toxin antitoxin (TA) system, where expression of the proteinaceous toxin (putative endonuclease) is controlled by an antisense sRNA.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262766. 164 interactors.
STRINGi511145.b4347.

Structurei

3D structure databases

ProteinModelPortaliP39394.
SMRiP39394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 74SpoVT-AbrBPROSITE-ProRule annotationAdd BLAST46

Sequence similaritiesi

Belongs to the SymE family.Curated
Contains 1 SpoVT-AbrB domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105FU6. Bacteria.
ENOG4111QQF. LUCA.
HOGENOMiHOG000266483.
KOiK19048.
OMAiQHTRTAH.

Family and domain databases

HAMAPiMF_01193. Endoribonucl_SymE. 1 hit.
InterProiIPR007159. SpoVT-AbrB_dom.
IPR014944. Toxin_SymE-like.
IPR020883. TypeI_TA_SymE.
[Graphical view]
PfamiPF08845. SymE_toxin. 1 hit.
[Graphical view]
PROSITEiPS51740. SPOVT_ABRB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTHSIAQP FEAEVSPANN RHVTVGYASR YPDYSRIPAI TLKGQWLEAA
60 70 80 90 100
GFATGTAVDV KVMEGCIVLT AQPPAAEESE LMQSLRQVCK LSARKQKQVQ
110
AFIGVIAGKQ KVA
Length:113
Mass (Da):12,203
Last modified:July 10, 2007 - v2
Checksum:i4C3C0CE696CE7D9D
GO

Sequence cautioni

The sequence AAA97244 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE78337 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97244.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77303.2.
AP009048 Genomic DNA. Translation: BAE78337.1. Different initiation.
PIRiS56573.
RefSeqiNP_418767.4. NC_000913.3.
WP_000132601.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77303; AAC77303; b4347.
BAE78337; BAE78337; BAE78337.
GeneIDi949088.
KEGGiecj:JW4310.
eco:b4347.
PATRICi32124300. VBIEscCol129921_4493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97244.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77303.2.
AP009048 Genomic DNA. Translation: BAE78337.1. Different initiation.
PIRiS56573.
RefSeqiNP_418767.4. NC_000913.3.
WP_000132601.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39394.
SMRiP39394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262766. 164 interactors.
STRINGi511145.b4347.

Proteomic databases

PaxDbiP39394.
PRIDEiP39394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77303; AAC77303; b4347.
BAE78337; BAE78337; BAE78337.
GeneIDi949088.
KEGGiecj:JW4310.
eco:b4347.
PATRICi32124300. VBIEscCol129921_4493.

Organism-specific databases

EchoBASEiEB2470.
EcoGeneiEG12584. symE.

Phylogenomic databases

eggNOGiENOG4105FU6. Bacteria.
ENOG4111QQF. LUCA.
HOGENOMiHOG000266483.
KOiK19048.
OMAiQHTRTAH.

Enzyme and pathway databases

BioCyciEcoCyc:G7940-MONOMER.
ECOL316407:JW4310-MONOMER.

Miscellaneous databases

PROiP39394.

Family and domain databases

HAMAPiMF_01193. Endoribonucl_SymE. 1 hit.
InterProiIPR007159. SpoVT-AbrB_dom.
IPR014944. Toxin_SymE-like.
IPR020883. TypeI_TA_SymE.
[Graphical view]
PfamiPF08845. SymE_toxin. 1 hit.
[Graphical view]
PROSITEiPS51740. SPOVT_ABRB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYME_ECOLI
AccessioniPrimary (citable) accession number: P39394
Secondary accession number(s): Q2M5W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of this protein is toxic for the cell, affecting colony-forming ability and protein synthesis.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.