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Protein

Multidrug resistance protein MdtM

Gene

mdtM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP.1 Publication

GO - Molecular functioni

  • drug:proton antiporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: EcoCyc
  • potassium:proton antiporter activity Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoCyc

GO - Biological processi

  • drug transmembrane transport Source: EcoCyc
  • regulation of cellular pH Source: EcoCyc
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJIO-MONOMER.
ECOL316407:JW4300-MONOMER.
MetaCyc:YJIO-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.52. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtM
Gene namesi
Name:mdtM
Synonyms:yjiO
Ordered Locus Names:b4337, JW4300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12576. mdtM.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 4816PeriplasmicSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 789CytoplasmicSequence analysis
Transmembranei79 – 9921HelicalSequence analysisAdd
BLAST
Topological domaini100 – 1034PeriplasmicSequence analysis
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 14016CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 1676PeriplasmicSequence analysis
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Topological domaini189 – 21628CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Topological domaini238 – 25114PeriplasmicSequence analysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence analysisAdd
BLAST
Topological domaini273 – 28210CytoplasmicSequence analysis
Transmembranei283 – 30321HelicalSequence analysisAdd
BLAST
Topological domaini304 – 3074PeriplasmicSequence analysis
Transmembranei308 – 32821HelicalSequence analysisAdd
BLAST
Topological domaini329 – 34820CytoplasmicSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST
Topological domaini370 – 3734PeriplasmicSequence analysis
Transmembranei374 – 39421HelicalSequence analysisAdd
BLAST
Topological domaini395 – 41016CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Multidrug resistance protein MdtMPRO_0000084839Add
BLAST

Proteomic databases

PaxDbiP39386.

Interactioni

Protein-protein interaction databases

BioGridi4261450. 183 interactions.
DIPiDIP-12641N.
STRINGi511145.b4337.

Structurei

3D structure databases

ProteinModelPortaliP39386.
SMRiP39386. Positions 12-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41083G8. Bacteria.
ENOG410XT98. LUCA.
HOGENOMiHOG000118215.
InParanoidiP39386.
OMAiAPCILIP.
OrthoDBiEOG6NPM4Z.
PhylomeDBiP39386.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRFFTRHAA TLFFPMALIL YDFAAYLSTD LIQPGIINVV RDFNADVSLA
60 70 80 90 100
PAAVSLYLAG GMALQWLLGP LSDRIGRRPV LITGALIFTL ACAATMFTTS
110 120 130 140 150
MTQFLIARAI QGTSICFIAT VGYVTVQEAF GQTKGIKLMA IITSIVLIAP
160 170 180 190 200
IIGPLSGAAL MHFMHWKVLF AIIAVMGFIS FVGLLLAMPE TVKRGAVPFS
210 220 230 240 250
AKSVLRDFRN VFCNRLFLFG AATISLSYIP MMSWVAVSPV ILIDAGSLTT
260 270 280 290 300
SQFAWTQVPV FGAVIVANAI VARFVKDPTE PRFIWRAVPI QLVGLSLLIV
310 320 330 340 350
GNLLSPHVWL WSVLGTSLYA FGIGLIFPTL FRFTLFSNKL PKGTVSASLN
360 370 380 390 400
MVILMVMSVS VEIGRWLWFN GGRLPFHLLA VVAGVIVVFT LAGLLNRVRQ
410
HQAAELVEEQ
Length:410
Mass (Da):44,688
Last modified:February 1, 1995 - v1
Checksum:i17C909971AD788B7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti128 – 1281E → K in strain: B.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY283773 Genomic DNA. Translation: AAP43530.1.
U14003 Genomic DNA. Translation: AAA97233.1.
U00096 Genomic DNA. Translation: AAC77293.1.
AP009048 Genomic DNA. Translation: BAE78330.1.
PIRiS56562.
RefSeqiNP_418757.1. NC_000913.3.
WP_001137036.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77293; AAC77293; b4337.
BAE78330; BAE78330; BAE78330.
GeneIDi948861.
KEGGiecj:JW4300.
eco:b4337.
PATRICi32124280. VBIEscCol129921_4483.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY283773 Genomic DNA. Translation: AAP43530.1.
U14003 Genomic DNA. Translation: AAA97233.1.
U00096 Genomic DNA. Translation: AAC77293.1.
AP009048 Genomic DNA. Translation: BAE78330.1.
PIRiS56562.
RefSeqiNP_418757.1. NC_000913.3.
WP_001137036.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39386.
SMRiP39386. Positions 12-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261450. 183 interactions.
DIPiDIP-12641N.
STRINGi511145.b4337.

Protein family/group databases

TCDBi2.A.1.2.52. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP39386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77293; AAC77293; b4337.
BAE78330; BAE78330; BAE78330.
GeneIDi948861.
KEGGiecj:JW4300.
eco:b4337.
PATRICi32124280. VBIEscCol129921_4483.

Organism-specific databases

EchoBASEiEB2464.
EcoGeneiEG12576. mdtM.

Phylogenomic databases

eggNOGiENOG41083G8. Bacteria.
ENOG410XT98. LUCA.
HOGENOMiHOG000118215.
InParanoidiP39386.
OMAiAPCILIP.
OrthoDBiEOG6NPM4Z.
PhylomeDBiP39386.

Enzyme and pathway databases

BioCyciEcoCyc:YJIO-MONOMER.
ECOL316407:JW4300-MONOMER.
MetaCyc:YJIO-MONOMER.

Miscellaneous databases

PROiP39386.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the YjiO gene of Escherichia coli B."
    Ramchandani J.H., Bhattacharjee S.K., Mahajan S.K.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Analysis of a complete library of putative drug transporter genes in Escherichia coli."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 183:5803-5812(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMDTM_ECOLI
AccessioniPrimary (citable) accession number: P39386
Secondary accession number(s): Q2M5X6, Q7X4V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.