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Protein

D-xylonate dehydratase YjhG

Gene

yjhG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of D-xylonic acid to form 2-dehydro-3-deoxy-D-pentonate.1 Publication1 Publication

Catalytic activityi

D-xylonate = 2-dehydro-3-deoxy-D-xylonate + H2O.1 Publication1 Publication

Enzyme regulationi

Activity is increased in the presence of Mn+ and Mg2+. Inhibited by thiol compounds.1 Publication

Kineticsi

kcat is 0.33 min(-1).1 Publication
  1. KM=4.88 mM for D-xylonate1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius.1 Publication

    GO - Molecular functioni

    • xylonate dehydratase activity Source: EcoCyc

    GO - Biological processi

    • aldonic acid catabolic process Source: EcoCyc

    Keywordsi

    Molecular functionLyase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7910-MONOMER.
    MetaCyc:G7910-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-xylonate dehydratase YjhG1 Publication (EC:4.2.1.821 Publication1 Publication)
    Gene namesi
    Name:yjhG
    Ordered Locus Names:b4297, JW4259
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12549. yjhG.

    Pathology & Biotechi

    Disruption phenotypei

    Disruption mutant has reduced ability to catabolize D-xylonic acid. YjhG-yagF double mutant cannot use D-xylonate as the sole source of carbon.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001035611 – 655D-xylonate dehydratase YjhGAdd BLAST655

    Proteomic databases

    PaxDbiP39358.
    PRIDEiP39358.

    Interactioni

    Protein-protein interaction databases

    BioGridi4260973. 10 interactors.
    IntActiP39358. 4 interactors.
    MINTiMINT-1250336.
    STRINGi316385.ECDH10B_4498.

    Structurei

    3D structure databases

    ProteinModelPortaliP39358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IlvD/Edd family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C01. Bacteria.
    COG0129. LUCA.
    HOGENOMiHOG000173155.
    PhylomeDBiP39358.

    Family and domain databases

    InterProiView protein in InterPro
    IPR017798. Dehydratase_YjhG/YagF.
    IPR000581. DiOHA_6PGluconate_deHydtase.
    IPR020558. DiOHA_6PGluconate_deHydtase_CS.
    IPR037237. IlvD/EDD_N.
    PfamiView protein in Pfam
    PF00920. ILVD_EDD. 1 hit.
    SUPFAMiSSF143975. SSF143975. 1 hit.
    TIGRFAMsiTIGR03432. yjhG_yagF. 1 hit.
    PROSITEiView protein in PROSITE
    PS00886. ILVD_EDD_1. 1 hit.
    PS00887. ILVD_EDD_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39358-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVRNIFADE SHDIYTVRTH ADGPDGELPL TAEMLINRPS GDLFGMTMNA
    60 70 80 90 100
    GMGWSPDELD RDGILLLSTL GGLRGADGKP VALALHQGHY ELDIQMKAAA
    110 120 130 140 150
    EVIKANHALP YAVYVSDPCD GRTQGTTGMF DSLPYRNDAS MVMRRLIRSL
    160 170 180 190 200
    PDAKAVIGVA SCDKGLPATM MALAAQHNIA TVLVPGGATL PAKDGEDNGK
    210 220 230 240 250
    VQTIGARFAN GELSLQDARR AGCKACASSG GGCQFLGTAG TSQVVAEGLG
    260 270 280 290 300
    LAIPHSALAP SGEPVWREIA RASARAALNL SQKGITTREI LTDKAIENAM
    310 320 330 340 350
    TVHAAFGGST NLLLHIPAIA HQAGCHIPTV DDWIRINKRV PRLVSVLPNG
    360 370 380 390 400
    PVYHPTVNAF MAGGVPEVML HLRSLGLLHE DVMTVTGSTL KENLDWWEHS
    410 420 430 440 450
    ERRQRFKQLL LDQEQINADE VIMSPQQAKA RGLTSTITFP VGNIAPEGSV
    460 470 480 490 500
    IKSTAIDPSM IDEQGIYYHK GVAKVYLSEK SAIYDIKHDK IKAGDILVII
    510 520 530 540 550
    GVGPSGTGME ETYQVTSALK HLSYGKHVSL ITDARFSGVS TGACIGHVGP
    560 570 580 590 600
    EALAGGPIGK LRTGDLIEIK IDCRELHGEV NFLGTRSDEQ LPSQEEATAI
    610 620 630 640 650
    LNARPSHQDL LPDPELPDDT RLWAMLQAVS GGTWTGCIYD VNKIGAALRD

    FMNKN
    Length:655
    Mass (Da):70,017
    Last modified:July 15, 1998 - v2
    Checksum:i95F88200CB6EF14C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti555 – 556GG → EA in AAA97193 (PubMed:7610040).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97193.1.
    U00096 Genomic DNA. Translation: AAC77253.1.
    AP009048 Genomic DNA. Translation: BAE78288.1.
    PIRiC65243.
    RefSeqiNP_418717.1. NC_000913.3.
    WP_000116326.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77253; AAC77253; b4297.
    BAE78288; BAE78288; BAE78288.
    GeneIDi946829.
    KEGGiecj:JW4259.
    eco:b4297.
    PATRICifig|1411691.4.peg.2400.

    Similar proteinsi

    Entry informationi

    Entry nameiYJHG_ECOLI
    AccessioniPrimary (citable) accession number: P39358
    Secondary accession number(s): Q2M618
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 15, 1998
    Last modified: November 22, 2017
    This is version 123 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families