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Protein

L-idonate 5-dehydrogenase (NAD(P)(+))

Gene

idnD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADH/NADPH-dependent oxidation of L-idonate to 5-ketogluconate (5KG).1 Publication

Catalytic activityi

L-idonate + NAD(P)+ = 5-dehydrogluconate + NAD(P)H.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: L-idonate degradation

This protein is involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the pathway L-idonate degradation and in Carbohydrate acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Zinc 1; catalyticBy similarity
Metal bindingi65 – 651Zinc 1; catalyticBy similarity
Metal bindingi93 – 931Zinc 2By similarity
Metal bindingi96 – 961Zinc 2By similarity
Metal bindingi99 – 991Zinc 2By similarity
Metal bindingi107 – 1071Zinc 2By similarity
Metal bindingi153 – 1531Zinc 1; catalyticBy similarity

GO - Molecular functioni

  • L-idonate 5-dehydrogenase activity Source: EcoCyc
  • zinc ion binding Source: InterPro

GO - Biological processi

  • D-gluconate metabolic process Source: UniProtKB-KW
  • L-idonate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Gluconate utilization

Keywords - Ligandi

Metal-binding, NADP, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:IDONDEHYD-MONOMER.
ECOL316407:JW4224-MONOMER.
MetaCyc:IDONDEHYD-MONOMER.
RETL1328306-WGS:GSTH-4318-MONOMER.
RETL1328306-WGS:GSTH-6752-MONOMER.
BRENDAi1.1.1.264. 2026.
UniPathwayiUPA00793.

Names & Taxonomyi

Protein namesi
Recommended name:
L-idonate 5-dehydrogenase (NAD(P)(+)) (EC:1.1.1.264)
Gene namesi
Name:idnD
Synonyms:yjgV
Ordered Locus Names:b4267, JW4224
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12541. idnD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343L-idonate 5-dehydrogenase (NAD(P)(+))PRO_0000160881Add
BLAST

Proteomic databases

PaxDbiP39346.

Interactioni

Protein-protein interaction databases

BioGridi4259666. 722 interactions.
DIPiDIP-10010N.
IntActiP39346. 5 interactions.
MINTiMINT-1242659.
STRINGi511145.b4267.

Structurei

3D structure databases

ProteinModelPortaliP39346.
SMRiP39346. Positions 32-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294670.
InParanoidiP39346.
KOiK00098.
OMAiAERWQCH.
OrthoDBiEOG6MWN7S.
PhylomeDBiP39346.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P39346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVKTQSCVV AGKKTVAVTE QTIDWNNNGT LVQITRGGIC GSDLHYYQEG
60 70 80 90 100
KVGNFMIKAP MVLGHEVIGK VIHSDSSELH EGQTVAINPS KPCGHCKYCI
110 120 130 140 150
EHNENQCTDM RFFGSAMYFP HVDGGFTRYK MVETSQCVPY PAKADEKVMA
160 170 180 190 200
FAEPLAVAIH AAHQAGELQG KRVFISGVGP IGCLIVSAVK TLGAAEIVCA
210 220 230 240 250
DVSPRSLSLG KEMGADVLVN PQNDDMDHWK AEKGYFDVSF EVSGHPSSVN
260 270 280 290 300
TCLEVTRARG VMVQVGMGGA MAEFPMMTLI GKEISLRGSF RFTSEFNTAV
310 320 330 340
SWLANGVINP LPLLSAEYPF TDLEEALRFA GDKTQAAKVQ LVF
Length:343
Mass (Da):37,147
Last modified:February 1, 1995 - v1
Checksum:i7A3C6E6C0CED6F09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97164.1.
U00096 Genomic DNA. Translation: AAC77224.1.
AP009048 Genomic DNA. Translation: BAE78264.1.
PIRiS56493.
RefSeqiNP_418688.1. NC_000913.3.
WP_001197411.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77224; AAC77224; b4267.
BAE78264; BAE78264; BAE78264.
GeneIDi944769.
KEGGiecj:JW4224.
eco:b4267.
PATRICi32124107. VBIEscCol129921_4398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97164.1.
U00096 Genomic DNA. Translation: AAC77224.1.
AP009048 Genomic DNA. Translation: BAE78264.1.
PIRiS56493.
RefSeqiNP_418688.1. NC_000913.3.
WP_001197411.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39346.
SMRiP39346. Positions 32-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259666. 722 interactions.
DIPiDIP-10010N.
IntActiP39346. 5 interactions.
MINTiMINT-1242659.
STRINGi511145.b4267.

Proteomic databases

PaxDbiP39346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77224; AAC77224; b4267.
BAE78264; BAE78264; BAE78264.
GeneIDi944769.
KEGGiecj:JW4224.
eco:b4267.
PATRICi32124107. VBIEscCol129921_4398.

Organism-specific databases

EchoBASEiEB2430.
EcoGeneiEG12541. idnD.

Phylogenomic databases

eggNOGiENOG4105CPQ. Bacteria.
COG1063. LUCA.
HOGENOMiHOG000294670.
InParanoidiP39346.
KOiK00098.
OMAiAERWQCH.
OrthoDBiEOG6MWN7S.
PhylomeDBiP39346.

Enzyme and pathway databases

UniPathwayiUPA00793.
BioCyciEcoCyc:IDONDEHYD-MONOMER.
ECOL316407:JW4224-MONOMER.
MetaCyc:IDONDEHYD-MONOMER.
RETL1328306-WGS:GSTH-4318-MONOMER.
RETL1328306-WGS:GSTH-6752-MONOMER.
BRENDAi1.1.1.264. 2026.

Miscellaneous databases

PROiP39346.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli."
    Bausch C., Peekhaus N., Utz C., Blais T., Murray E., Lowary T., Conway T.
    J. Bacteriol. 180:3704-3710(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiIDND_ECOLI
AccessioniPrimary (citable) accession number: P39346
Secondary accession number(s): Q2M642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 20, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.