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Protein

Cyclic-di-GMP-binding biofilm dispersal mediator protein

Gene

bdcA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Increases biofilm dispersal. Acts by binding directly to the signaling molecule cyclic-di-GMP, which decreases the intracellular concentration of cyclic-di-GMP and leads to biofilm dispersal. Also controls other biofilm-related phenotypes such as cell motility, cell size, cell aggregation and production of extracellular DNA and extracellular polysaccharides (EPS). Does not act as a phosphodiesterase.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132SubstrateBy similarity1
Active sitei146Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 34NAD or NADPBy similarityAdd BLAST25

GO - Molecular functioni

  • cyclic-di-GMP binding Source: EcoCyc
  • NADPH binding Source: EcoCyc
  • oxidoreductase activity Source: InterPro

GO - Biological processi

  • single-species biofilm formation Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

c-di-GMP

Enzyme and pathway databases

BioCyciEcoCyc:G7880-MONOMER.
ECOL316407:JW4207-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic-di-GMP-binding biofilm dispersal mediator protein
Gene namesi
Name:bdcA
Synonyms:yjgI
Ordered Locus Names:b4249, JW4207
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12528. bdcA.

Pathology & Biotechi

Disruption phenotypei

Mutant shows decreased biofilm dispersal. Deletion increases the cyclic-di GMP concentration in the cell.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50E → Q or V: Shows higher affinity for cyclic-di-GMP, increases swimming motility and biofilm dispersal. Biofilm formation is almost completely removed. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548391 – 237Cyclic-di-GMP-binding biofilm dispersal mediator proteinAdd BLAST237

Proteomic databases

PaxDbiP39333.
PRIDEiP39333.

Expressioni

Inductioni

Expression is time dependent in biofilms and is controlled by BdcR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259560. 12 interactors.
DIPiDIP-12604N.
IntActiP39333. 1 interactor.
STRINGi511145.b4249.

Structurei

3D structure databases

ProteinModelPortaliP39333.
SMRiP39333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F6U. Bacteria.
COG1028. LUCA.
InParanoidiP39333.
KOiK19336.
OMAiTWYCALE.
PhylomeDBiP39333.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAFTGKTVL ILGGSRGIGA AIVRRFVTDG ANVRFTYAGS KDAAKRLAQE
60 70 80 90 100
TGATAVFTDS ADRDAVIDVV RKSGALDILV VNAGIGVFGE ALELNADDID
110 120 130 140 150
RLFKINIHAP YHASVEAARQ MPEGGRILII GSVNGDRMPV AGMAAYAASK
160 170 180 190 200
SALQGMARGL ARDFGPRGIT INVVQPGPID TDANPANGPM RDMLHSLMAI
210 220 230
KRHGQPEEVA GMVAWLAGPE ASFVTGAMHT IDGAFGA
Length:237
Mass (Da):24,598
Last modified:July 15, 1998 - v2
Checksum:iA99A3F23CE0116C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97146.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC77206.1.
AP009048 Genomic DNA. Translation: BAE78247.1.
PIRiD65237.
RefSeqiNP_418670.1. NC_000913.3.
WP_000500727.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC77206; AAC77206; b4249.
BAE78247; BAE78247; BAE78247.
GeneIDi948765.
KEGGiecj:JW4207.
eco:b4249.
PATRICi32124071. VBIEscCol129921_4380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97146.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC77206.1.
AP009048 Genomic DNA. Translation: BAE78247.1.
PIRiD65237.
RefSeqiNP_418670.1. NC_000913.3.
WP_000500727.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP39333.
SMRiP39333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259560. 12 interactors.
DIPiDIP-12604N.
IntActiP39333. 1 interactor.
STRINGi511145.b4249.

Proteomic databases

PaxDbiP39333.
PRIDEiP39333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77206; AAC77206; b4249.
BAE78247; BAE78247; BAE78247.
GeneIDi948765.
KEGGiecj:JW4207.
eco:b4249.
PATRICi32124071. VBIEscCol129921_4380.

Organism-specific databases

EchoBASEiEB2418.
EcoGeneiEG12528. bdcA.

Phylogenomic databases

eggNOGiENOG4105F6U. Bacteria.
COG1028. LUCA.
InParanoidiP39333.
KOiK19336.
OMAiTWYCALE.
PhylomeDBiP39333.

Enzyme and pathway databases

BioCyciEcoCyc:G7880-MONOMER.
ECOL316407:JW4207-MONOMER.

Miscellaneous databases

PROiP39333.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBDCA_ECOLI
AccessioniPrimary (citable) accession number: P39333
Secondary accession number(s): Q2M659
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.