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Protein

Translocation and assembly module TamB

Gene

tamB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the translocation and assembly module (TAM) autotransporter assembly complex, which functions in translocation of autotransporters across the outer membrane.1 Publication

GO - Biological processi

  • protein secretion Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7875-MONOMER.
ECOL316407:JW4180-MONOMER.

Protein family/group databases

TCDBi9.B.22.1.2. the leukotoxin secretion morphogenesis protein c (morc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocation and assembly module TamB
Alternative name(s):
Autotransporter assembly factor TamB
Gene namesi
Name:tamB
Synonyms:ytfN, ytfO
Ordered Locus Names:b4221, JW4180
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12514. tamB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicBy similarity
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinBy similarityAdd
BLAST
Topological domaini28 – 12591232PeriplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • TAM protein secretion complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss of localization of an exogenous protein, P1121 (ROD_p1121 of Citrobacter rodentium), to outer membranes, loss of cell aggregation when adhesins are over-expressed in a double tamA-tamB deletion mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12591259Translocation and assembly module TamBPRO_0000169830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine1 Publication

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP39321.
PRIDEiP39321.

Interactioni

Subunit structurei

Interacts with TamA.1 Publication

Protein-protein interaction databases

BioGridi4259309. 311 interactions.
DIPiDIP-12942N.
IntActiP39321. 6 interactions.
STRINGi511145.b4221.

Structurei

3D structure databases

ProteinModelPortaliP39321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TamB family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105MA0. Bacteria.
COG2911. LUCA.
HOGENOMiHOG000127429.
InParanoidiP39321.
KOiK09800.
OMAiKGNEQQI.
OrthoDBiEOG6GJBQK.
PhylomeDBiP39321.

Family and domain databases

InterProiIPR007452. TamB.
[Graphical view]
PfamiPF04357. TamB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWKKISLG VVIVILLLLG SVAFLVGTTS GLHLVFKAAD RWVPGLDIGK
60 70 80 90 100
VTGGWRDLTL SDVRYEQPGV AVKAGNLHLA VGLECLWNSS VCINDLALKD
110 120 130 140 150
IQVNIDSKKM PPSEQVEEEE DSGPLDLSTP YPITLTRVAL DNVNIKIDDT
160 170 180 190 200
TVSVMDFTSG LNWQEKTLTL KPTSLKGLLI ALPKVAEVAQ EEVVEPKIEN
210 220 230 240 250
PQPDEKPLGE TLKDLFSRPV LPEMTDVHLP LNLNIEEFKG EQLRVTGDTD
260 270 280 290 300
ITVSTMLLKV SSIDGNTKLD ALDIDSSQGI VNASGTAQLS DNWPVDITLN
310 320 330 340 350
STLNVEPLKG EKVKLKMGGA LREQLEIGVN LSGPVDMDLR AQTRLAEAGL
360 370 380 390 400
PLNVEVNSKQ LYWPFTGEKQ YQADDLKLKL TGKMTDYTLS MRTAVKGQEI
410 420 430 440 450
PPATITLDAK GNEQQVNLDK LTVAALEGKT ELKALLDWQQ AISWRGELTL
460 470 480 490 500
NGINTAKEFP DWPSKLNGLI KTRGSLYGGT WQMDVPELKL TGNVKQNKVN
510 520 530 540 550
VDGTLKGNSY MQWMIPGLHL ELGPNSAEVK GELGVKDLNL DATINAPGLD
560 570 580 590 600
NALPGLGGTA KGLVKVRGTV EAPQLLADIT ARGLRWQELS VAQVRVEGDI
610 620 630 640 650
KSTDQIAGKL DVRVEQISQP DVNINLVTLN AKGSEKQHEL QLRIQGEPVS
660 670 680 690 700
GQLNLAGSFD RKEERWKGTL SNTRFQTPVG PWSLTRDIAL DYRNKEQKIS
710 720 730 740 750
IGPHCWLNPN AELCVPQTID AGAEGRAVVN LNRFDLAMLK PFMPETTQAS
760 770 780 790 800
GIFTGKADVA WDTTKEGLPQ GSITLSGRNV QVTQTVNDAA LPVAFQTLNL
810 820 830 840 850
TAELRNNRAE LGWTIRLTNN GQFDGQVQVT DPQGRRNLGG NVNIRNFNLA
860 870 880 890 900
MINPIFTRGE KAAGMVSANL RLGGDVQSPQ LFGQLQVTGV DIDGNFMPFD
910 920 930 940 950
MQPSQLAVNF NGMRSTLAGT VRTQQGEIYL NGDADWSQIE NWRARVTAKG
960 970 980 990 1000
SKVRITVPPM VRMDVSPDVV FEATPNLFTL DGRVDVPWAR IVVHDLPESA
1010 1020 1030 1040 1050
VGVSSDVVML NDNLQPEEPK TASIPINSNL IVHVGNNVRI DAFGLKARLT
1060 1070 1080 1090 1100
GDLNVVQDKQ GLGLNGQINI PEGRFHAYGQ DLIVRKGELL FSGPPDQPYL
1110 1120 1130 1140 1150
NIEAIRNPDA TEDDVIAGVR VTGLADEPKA EIFSDPAMSQ QAALSYLLRG
1160 1170 1180 1190 1200
QGLESDQSDS AAMTSMLIGL GVAQSGQIVG KIGETFGVSN LALDTQGVGD
1210 1220 1230 1240 1250
SSQVVVSGYV LPGLQVKYGV GIFDSIATLT LRYRLMPKLY LEAVSGVDQA

LDLLYQFEF
Length:1,259
Mass (Da):136,780
Last modified:November 1, 1997 - v2
Checksum:iCDBB43F0F8C81DB1
GO

Sequence cautioni

The sequence AAA97118.1 differs from that shown. Reason: Frameshift at position 493. Produces two separate ORFs.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97117.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97118.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77178.1.
AP009048 Genomic DNA. Translation: BAE78222.1.
PIRiH65233.
RefSeqiNP_418642.1. NC_000913.3.
WP_000060911.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC77178; AAC77178; b4221.
BAE78222; BAE78222; BAE78222.
GeneIDi948742.
KEGGiecj:JW4180.
eco:b4221.
PATRICi32124019. VBIEscCol129921_4354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97117.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97118.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77178.1.
AP009048 Genomic DNA. Translation: BAE78222.1.
PIRiH65233.
RefSeqiNP_418642.1. NC_000913.3.
WP_000060911.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP39321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259309. 311 interactions.
DIPiDIP-12942N.
IntActiP39321. 6 interactions.
STRINGi511145.b4221.

Protein family/group databases

TCDBi9.B.22.1.2. the leukotoxin secretion morphogenesis protein c (morc) family.

Proteomic databases

PaxDbiP39321.
PRIDEiP39321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77178; AAC77178; b4221.
BAE78222; BAE78222; BAE78222.
GeneIDi948742.
KEGGiecj:JW4180.
eco:b4221.
PATRICi32124019. VBIEscCol129921_4354.

Organism-specific databases

EchoBASEiEB2407.
EcoGeneiEG12514. tamB.

Phylogenomic databases

eggNOGiENOG4105MA0. Bacteria.
COG2911. LUCA.
HOGENOMiHOG000127429.
InParanoidiP39321.
KOiK09800.
OMAiKGNEQQI.
OrthoDBiEOG6GJBQK.
PhylomeDBiP39321.

Enzyme and pathway databases

BioCyciEcoCyc:G7875-MONOMER.
ECOL316407:JW4180-MONOMER.

Miscellaneous databases

PROiP39321.

Family and domain databases

InterProiIPR007452. TamB.
[Graphical view]
PfamiPF04357. TamB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: PROTEIN SEQUENCE OF 1-6, FUNCTION IN PROTEIN TRANSLOCATION, SUBCELLULAR LOCATION, SUBUNIT, FORMYLATION AT MET-1, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiTAMB_ECOLI
AccessioniPrimary (citable) accession number: P39321
Secondary accession number(s): P39322, P76802, Q2M684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.