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Protein

Translocation and assembly module subunit TamB

Gene

tamB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the translocation and assembly module (TAM) autotransporter assembly complex, which functions in translocation of autotransporters across the outer membrane (PubMed:22466966). In reconstituted TAM this subunit (Ag43, AC P39180) is not necessary for substrate penetration in the outer membrane. Substrate binding to TamA moves its POTRA domains about 30 Angstroms into the periplasm, which would deform either the outer membrane or TamB and may provide force to reset TAM (PubMed:25341963).2 Publications

GO - Biological processi

  • protein secretion Source: EcoCyc

Enzyme and pathway databases

BioCyciEcoCyc:G7875-MONOMER

Protein family/group databases

TCDBi9.B.22.1.2 the leukotoxin secretion morphogenesis protein c (morc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Translocation and assembly module subunit TamB1 Publication
Alternative name(s):
Autotransporter assembly factor TamB
Gene namesi
Name:tamB1 Publication
Synonyms:ytfN, ytfO
Ordered Locus Names:b4221, JW4180
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12514 tamB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicBy similarity6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinBy similarityAdd BLAST21
Topological domaini28 – 1259Periplasmic1 PublicationAdd BLAST1232

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • TAM protein secretion complex Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss of localization of an exogenous protein, P1121 (ROD_p1121 of Citrobacter rodentium), to outer membranes, loss of cell aggregation when adhesins are over-expressed in a double tamA-tamB deletion mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698301 – 1259Translocation and assembly module subunit TamBAdd BLAST1259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-formylmethionine1 Publication1

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP39321
PRIDEiP39321

Interactioni

Subunit structurei

Interacts with TamA to form the translocation and assembly module (TAM).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
tamAP0ADE42EBI-1117885,EBI-1114298

Protein-protein interaction databases

BioGridi4259309, 404 interactors
DIPiDIP-12942N
IntActiP39321, 7 interactors
STRINGi316385.ECDH10B_4416

Structurei

Secondary structure

11259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi977 – 993Combined sources17
Beta strandi1026 – 1042Combined sources17
Beta strandi1045 – 1058Combined sources14
Beta strandi1061 – 1077Combined sources17
Beta strandi1080 – 1093Combined sources14
Beta strandi1099 – 1105Combined sources7
Beta strandi1111 – 1113Combined sources3
Beta strandi1118 – 1124Combined sources7
Beta strandi1129 – 1135Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VTGX-ray1.86A/B963-1138[»]
ProteinModelPortaliP39321
SMRiP39321
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The periplasmic domain is elongated (up to 160 Angstroms long) and forms contacts with the N-terminal POTRA domains of TamA (PubMed:25341963).1 Publication

Sequence similaritiesi

Belongs to the TamB family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105MA0 Bacteria
COG2911 LUCA
HOGENOMiHOG000127429
InParanoidiP39321
KOiK09800
OMAiRYRAYGQ
PhylomeDBiP39321

Family and domain databases

InterProiView protein in InterPro
IPR007452 TamB
PfamiView protein in Pfam
PF04357 TamB, 1 hit

Sequencei

Sequence statusi: Complete.

P39321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWKKISLG VVIVILLLLG SVAFLVGTTS GLHLVFKAAD RWVPGLDIGK
60 70 80 90 100
VTGGWRDLTL SDVRYEQPGV AVKAGNLHLA VGLECLWNSS VCINDLALKD
110 120 130 140 150
IQVNIDSKKM PPSEQVEEEE DSGPLDLSTP YPITLTRVAL DNVNIKIDDT
160 170 180 190 200
TVSVMDFTSG LNWQEKTLTL KPTSLKGLLI ALPKVAEVAQ EEVVEPKIEN
210 220 230 240 250
PQPDEKPLGE TLKDLFSRPV LPEMTDVHLP LNLNIEEFKG EQLRVTGDTD
260 270 280 290 300
ITVSTMLLKV SSIDGNTKLD ALDIDSSQGI VNASGTAQLS DNWPVDITLN
310 320 330 340 350
STLNVEPLKG EKVKLKMGGA LREQLEIGVN LSGPVDMDLR AQTRLAEAGL
360 370 380 390 400
PLNVEVNSKQ LYWPFTGEKQ YQADDLKLKL TGKMTDYTLS MRTAVKGQEI
410 420 430 440 450
PPATITLDAK GNEQQVNLDK LTVAALEGKT ELKALLDWQQ AISWRGELTL
460 470 480 490 500
NGINTAKEFP DWPSKLNGLI KTRGSLYGGT WQMDVPELKL TGNVKQNKVN
510 520 530 540 550
VDGTLKGNSY MQWMIPGLHL ELGPNSAEVK GELGVKDLNL DATINAPGLD
560 570 580 590 600
NALPGLGGTA KGLVKVRGTV EAPQLLADIT ARGLRWQELS VAQVRVEGDI
610 620 630 640 650
KSTDQIAGKL DVRVEQISQP DVNINLVTLN AKGSEKQHEL QLRIQGEPVS
660 670 680 690 700
GQLNLAGSFD RKEERWKGTL SNTRFQTPVG PWSLTRDIAL DYRNKEQKIS
710 720 730 740 750
IGPHCWLNPN AELCVPQTID AGAEGRAVVN LNRFDLAMLK PFMPETTQAS
760 770 780 790 800
GIFTGKADVA WDTTKEGLPQ GSITLSGRNV QVTQTVNDAA LPVAFQTLNL
810 820 830 840 850
TAELRNNRAE LGWTIRLTNN GQFDGQVQVT DPQGRRNLGG NVNIRNFNLA
860 870 880 890 900
MINPIFTRGE KAAGMVSANL RLGGDVQSPQ LFGQLQVTGV DIDGNFMPFD
910 920 930 940 950
MQPSQLAVNF NGMRSTLAGT VRTQQGEIYL NGDADWSQIE NWRARVTAKG
960 970 980 990 1000
SKVRITVPPM VRMDVSPDVV FEATPNLFTL DGRVDVPWAR IVVHDLPESA
1010 1020 1030 1040 1050
VGVSSDVVML NDNLQPEEPK TASIPINSNL IVHVGNNVRI DAFGLKARLT
1060 1070 1080 1090 1100
GDLNVVQDKQ GLGLNGQINI PEGRFHAYGQ DLIVRKGELL FSGPPDQPYL
1110 1120 1130 1140 1150
NIEAIRNPDA TEDDVIAGVR VTGLADEPKA EIFSDPAMSQ QAALSYLLRG
1160 1170 1180 1190 1200
QGLESDQSDS AAMTSMLIGL GVAQSGQIVG KIGETFGVSN LALDTQGVGD
1210 1220 1230 1240 1250
SSQVVVSGYV LPGLQVKYGV GIFDSIATLT LRYRLMPKLY LEAVSGVDQA

LDLLYQFEF
Length:1,259
Mass (Da):136,780
Last modified:November 1, 1997 - v2
Checksum:iCDBB43F0F8C81DB1
GO

Sequence cautioni

The sequence AAA97118 differs from that shown. Reason: Frameshift at position 493. Produces two separate ORFs.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97117.1 Frameshift.
U14003 Genomic DNA Translation: AAA97118.1 Frameshift.
U00096 Genomic DNA Translation: AAC77178.1
AP009048 Genomic DNA Translation: BAE78222.1
PIRiH65233
RefSeqiNP_418642.1, NC_000913.3
WP_000060911.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC77178; AAC77178; b4221
BAE78222; BAE78222; BAE78222
GeneIDi948742
KEGGiecj:JW4180
eco:b4221
PATRICifig|1411691.4.peg.2479

Similar proteinsi

Entry informationi

Entry nameiTAMB_ECOLI
AccessioniPrimary (citable) accession number: P39321
Secondary accession number(s): P39322, P76802, Q2M684
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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