Reviewed,
UniProtKB/Swiss-Prot P39306 (ULAF_ECOLI)
Last modified
November 3, 2009.
Version 78.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: L-ribulose-5-phosphate 4-epimerase ulaF EC=5.1.3.4 Alternative name(s): Phosphoribulose isomerase L-ascorbate utilization protein F | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 228 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization. Ref.5 |
| Catalytic activity | L-ribulose 5-phosphate = D-xylulose 5-phosphate. HAMAP MF_01952 |
| Cofactor | Binds 1 zinc ion per subunit Potential. |
| Pathway | Cofactor degradation; L-ascorbate degradation; D-xylulose 5-phosphate from L-ascorbate: step 4/4. HAMAP MF_01952 |
| Induction | Induced by L-ascorbate. Repressed by ulaR. HAMAP MF_01952 |
| Sequence similarities | Belongs to the aldolase class II family. AraD/fucA subfamily. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding Zinc |
| Molecular function | Isomerase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | L-ascorbic acid metabolic process Inferred from electronic annotation. Source: HAMAP |
| Molecular function | L-ribulose-phosphate 4-epimerase activity Inferred from electronic annotation. Source: EC zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 228 | 228 | L-ribulose-5-phosphate 4-epimerase ulaF HAMAP MF_01952 | PRO_0000162921 | |||||
Sites | |||||||||
| Metal binding | 74 | 1 | Zinc By similarity | ||||||
| Metal binding | 93 | 1 | Zinc By similarity | ||||||
| Metal binding | 95 | 1 | Zinc By similarity | ||||||
| Metal binding | 167 | 1 | Zinc By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes." Reizer J., Charbit A., Reizer A., Saier M.H. Jr. Genome Sci. Technol. 1:53-75(1996) Cited for: DISCUSSION OF SEQUENCE. |
| [5] | "Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons." Yew W.S., Gerlt J.A. J. Bacteriol. 184:302-306(2002) [PubMed: 11741871] [Abstract] Cited for: FUNCTION, CHARACTERIZATION. Strain: K12 / MG1655 / ATCC 47076. |
| [6] | "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli." Campos E., Aguilar J., Baldoma L., Badia J. J. Bacteriol. 184:6065-6068(2002) [PubMed: 12374842] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION. |
| [7] | "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli." Campos E., Baldoma L., Aguilar J., Badia J. J. Bacteriol. 186:1720-1728(2004) [PubMed: 14996803] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION. |
Cross-references
Sequence databases | |
|---|---|
| U14003 Genomic DNA. Translation: AAA97094.1. U00096 Genomic DNA. Translation: AAC77155.1. AP009048 Genomic DNA. Translation: BAE78199.1. | |
| PIR | S56423. |
| RefSeq | AP_004698.1. NP_418619.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K0W based on UniProtKB P08203. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:10868N. |
| STRING | P39306. |
Genome annotation databases | |
| GeneID | 948711. |
| GenomeReviews | Gene locus JW4156 in contig AP009048_GR. Gene locus b4198 in contig U00096_GR. |
| KEGG | ecj:JW4156. eco:b4198. |
Organism-specific databases | |
| EchoBASE | EB2391. |
| EcoGene | EG12498. ulaF. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P39306. |
| OMA | DAEPLHT. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:G7860-MON. MetaCyc:G7860-MON. |
Gene expression databases | |
| Genevestigator | P39306. |
Family and domain databases | |
| HAMAP | MF_01952. [Tree] |
| InterPro | IPR001303. Aldolase_II/adducin_N. [Graphical view] |
| Gene3D | G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit. |
| Pfam | PF00596. Aldolase_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ULAF_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P39306 Secondary accession number(s): Q2M6A7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


