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Protein

Ascorbate-specific permease IIC component UlaA

Gene

ulaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • L-ascorbic acid transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SGAT-MONOMER.
ECOL316407:JW5744-MONOMER.
MetaCyc:SGAT-MONOMER.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ascorbate-specific permease IIC component UlaA
Alternative name(s):
Ascorbate-specific PTS system EIIC component
Gene namesi
Name:ulaA
Synonyms:sgaT, yjfS
Ordered Locus Names:b4193, JW5744
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12493. ulaA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence analysisAdd
BLAST
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Transmembranei379 – 39921HelicalSequence analysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Ascorbate-specific permease IIC component UlaAPRO_0000097714Add
BLAST

Proteomic databases

PaxDbiP39301.

Expressioni

Inductioni

Induced by L-ascorbate. Repressed by UlaR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4262713. 6 interactions.
DIPiDIP-10870N.
IntActiP39301. 1 interaction.
MINTiMINT-1255643.
STRINGi511145.b4193.

Structurei

Secondary structure

1
465
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni8 – 103Combined sources
Helixi11 – 144Combined sources
Turni15 – 173Combined sources
Helixi19 – 3315Combined sources
Helixi38 – 7639Combined sources
Beta strandi81 – 844Combined sources
Helixi86 – 9712Combined sources
Helixi98 – 1014Combined sources
Helixi102 – 12019Combined sources
Helixi122 – 1254Combined sources
Beta strandi129 – 1313Combined sources
Helixi134 – 15017Combined sources
Helixi155 – 18531Combined sources
Helixi197 – 20610Combined sources
Helixi207 – 2093Combined sources
Helixi213 – 2153Combined sources
Turni217 – 2193Combined sources
Helixi224 – 2307Combined sources
Helixi232 – 24918Combined sources
Helixi253 – 2608Combined sources
Helixi265 – 30440Combined sources
Beta strandi310 – 3134Combined sources
Helixi315 – 3217Combined sources
Helixi323 – 34624Combined sources
Beta strandi353 – 3553Combined sources
Helixi357 – 40246Combined sources
Beta strandi405 – 4073Combined sources
Helixi412 – 4154Combined sources
Helixi417 – 42711Combined sources
Helixi428 – 4303Combined sources
Helixi431 – 45525Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RP8X-ray2.36A/C1-465[»]
4RP9X-ray1.65A1-465[»]
ProteinModelPortaliP39301.
SMRiP39301. Positions 6-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

In classical PTS systems, the EIIC domain forms the translocation channel and contains the specific substrate-binding site. UlaA does not exhibit the topological features of any recognized enzyme IIC.

Sequence similaritiesi

Belongs to the UlaA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CZC. Bacteria.
COG3037. LUCA.
HOGENOMiHOG000218566.
InParanoidiP39301.
KOiK03475.
OMAiPNSFGDE.
OrthoDBiEOG64JFNM.
PhylomeDBiP39301.

Family and domain databases

InterProiIPR004703. PTS_sugar-sp_permease.
[Graphical view]
PfamiPF03611. EIIC-GAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEILYNIFTV FFNQVMTNAP LLLGIVTCLG YILLRKSVSV IIKGTIKTII
60 70 80 90 100
GFMLLQAGSG ILTSTFKPVV AKMSEVYGIN GAISDTYASM MATIDRMGDA
110 120 130 140 150
YSWVGYAVLL ALALNICYVL LRRITGIRTI MLTGHIMFQQ AGLIAVTLFI
160 170 180 190 200
FGYSMWTTII CTAILVSLYW GITSNMMYKP TQEVTDGCGF SIGHQQQFAS
210 220 230 240 250
WIAYKVAPFL GKKEESVEDL KLPGWLNIFH DNIVSTAIVM TIFFGAILLS
260 270 280 290 300
FGIDTVQAMA GKVHWTVYIL QTGFSFAVAI FIITQGVRMF VAELSEAFNG
310 320 330 340 350
ISQRLIPGAV LAIDCAAIYS FAPNAVVWGF MWGTIGQLIA VGILVACGSS
360 370 380 390 400
ILIIPGFIPM FFSNATIGVF ANHFGGWRAA LKICLVMGMI EIFGCVWAVK
410 420 430 440 450
LTGMSAWMGM ADWSILAPPM MQGFFSIGIA FMAVIIVIAL AYMFFAGRAL
460
RAEEDAEKQL AEQSA
Length:465
Mass (Da):50,737
Last modified:April 4, 2006 - v3
Checksum:iCCFF1C776FEA4716
GO

Sequence cautioni

The sequence AAA97089.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti451 – 46515RAEED…AEQSA → AQKKMQKNNWQNSLLNKEF in AAA97089 (PubMed:7610040).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97089.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77150.2.
AP009048 Genomic DNA. Translation: BAE78194.1.
PIRiD65230.
RefSeqiNP_418614.4. NC_000913.3.
WP_001350568.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77150; AAC77150; b4193.
BAE78194; BAE78194; BAE78194.
GeneIDi948717.
KEGGiecj:JW5744.
eco:b4193.
PATRICi32123961. VBIEscCol129921_4325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97089.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77150.2.
AP009048 Genomic DNA. Translation: BAE78194.1.
PIRiD65230.
RefSeqiNP_418614.4. NC_000913.3.
WP_001350568.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RP8X-ray2.36A/C1-465[»]
4RP9X-ray1.65A1-465[»]
ProteinModelPortaliP39301.
SMRiP39301. Positions 6-456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262713. 6 interactions.
DIPiDIP-10870N.
IntActiP39301. 1 interaction.
MINTiMINT-1255643.
STRINGi511145.b4193.

Protein family/group databases

TCDBi4.A.7.1.1. the pts l-ascorbate (l-asc) family.

Proteomic databases

PaxDbiP39301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77150; AAC77150; b4193.
BAE78194; BAE78194; BAE78194.
GeneIDi948717.
KEGGiecj:JW5744.
eco:b4193.
PATRICi32123961. VBIEscCol129921_4325.

Organism-specific databases

EchoBASEiEB2386.
EcoGeneiEG12493. ulaA.

Phylogenomic databases

eggNOGiENOG4105CZC. Bacteria.
COG3037. LUCA.
HOGENOMiHOG000218566.
InParanoidiP39301.
KOiK03475.
OMAiPNSFGDE.
OrthoDBiEOG64JFNM.
PhylomeDBiP39301.

Enzyme and pathway databases

BioCyciEcoCyc:SGAT-MONOMER.
ECOL316407:JW5744-MONOMER.
MetaCyc:SGAT-MONOMER.

Miscellaneous databases

PROiP39301.

Family and domain databases

InterProiIPR004703. PTS_sugar-sp_permease.
[Graphical view]
PfamiPF03611. EIIC-GAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO C-TERMINUS.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferases system genes revealed by bacterial genome analysis: operons encoding homologues of sugar-specific permease domains of the phosphotransferase system and pentose catabolic enzymes."
    Reizer J., Charbit A., Reizer A., Saier M.H. Jr.
    Genome Sci. Technol. 1:53-75(1996)
    Cited for: DISCUSSION OF SEQUENCE.
  5. "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli."
    Campos E., Aguilar J., Baldoma L., Badia J.
    J. Bacteriol. 184:6065-6068(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.
  6. Cited for: CHARACTERIZATION.
  7. "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli."
    Campos E., Baldoma L., Aguilar J., Badia J.
    J. Bacteriol. 186:1720-1728(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiULAA_ECOLI
AccessioniPrimary (citable) accession number: P39301
Secondary accession number(s): Q2M6B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 4, 2006
Last modified: January 20, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.