Probable L-ascorbate-6-phosphate lactonase UlaG - Escherichia coli (strain K12)

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P39300 (ULAG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable L-ascorbate-6-phosphate lactonase UlaG
EC=3.1.1.-

Alternative name(s):
L-ascorbate utilization protein G

Gene names

Name:	ulaG
Synonyms:	yjfR
Ordered Locus Names:	b4192, JW5868

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions. Also shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p-nitrophenyl phosphate (bis-pNPP). Ref.5 Ref.7
Catalytic activity	L-ascorbate 6-phosphate + H₂O = 3-dehydro-L-gulonate 6-phosphate. HAMAP-Rule MF_01266
Cofactor	A divalent metal cation Potential.
Pathway	Cofactor degradation; L-ascorbate degradation; D-xylulose 5-phosphate from L-ascorbate: step 1/4. HAMAP-Rule MF_01266
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_01266.
Induction	Induced by L-ascorbate. Repressed by UlaR. Ref.4 Ref.6
Sequence similarities	Belongs to the UlaG family.
Sequence caution	The sequence AAA97088.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-ascorbic acid catabolic process Inferred from mutant phenotype Ref.5. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-ascorbate 6-phosphate lactonase activity Inferred from direct assay PubMed 20359483. Source: EcoCyc carboxylesterase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from direct assay PubMed 20359483. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 354

354

Probable L-ascorbate-6-phosphate lactonase UlaG HAMAP-Rule MF_01266

PRO_0000169757

Secondary structure

354

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P39300 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 00DECD310615BF66
Show »

FASTA

354

40,061

        10         20         30         40         50         60 
MSKVKSITRE SWILSTFPEW GSWLNEEIEQ EQVAPGTFAM WWLGCTGIWL KSEGGTNVCV 

        70         80         90        100        110        120 
DFWCGTGKQS HGNPLMKQGH QMQRMAGVKK LQPNLRTTPF VLDPFAIRQI DAVLATHDHN 

       130        140        150        160        170        180 
DHIDVNVAAA VMQNCADDVP FIGPKTCVDL WIGWGVPKER CIVVKPGDVV KVKDIEIHAL 

       190        200        210        220        230        240 
DAFDRTALIT LPADQKAAGV LPDGMDDRAV NYLFKTPGGS LYHSGDSHYS NYYAKHGNEH 

       250        260        270        280        290        300 
QIDVALGSYG ENPRGITDKM TSADMLRMGE ALNAKVVIPF HHDIWSNFQA DPQEIRVLWE 

       310        320        330        340        350 
MKKDRLKYGF KPFIWQVGGK FTWPLDKDNF EYHYPRGFDD CFTIEPDLPF KSFL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli." Campos E., Aguilar J., Baldoma L., Badia J. J. Bacteriol. 184:6065-6068(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.
[5]	"The ascorbate transporter of Escherichia coli." Zhang Z., Aboulwafa M., Smith M.H., Saier M.H. Jr. J. Bacteriol. 185:2243-2250(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROBABLE FUNCTION, ROLE IN L-ASCORBATE UTILIZATION. Strain: K12 / BW25113.
[6]	"Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in L-ascorbate dissimilation in Escherichia coli." Campos E., Baldoma L., Aguilar J., Badia J. J. Bacteriol. 186:1720-1728(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.
[7]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U14003 Genomic DNA. Translation: AAA97088.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77149.2.
AP009048 Genomic DNA. Translation: BAE78193.1.

RefSeq

NP_418613.2. NC_000913.2.
YP_492334.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WYL	X-ray	2.59	A/B/C/D/E/F	1-354	[»]
2WYM	X-ray	2.60	A/B/C/D/E/F	1-354	[»]

ProteinModelPortal

P39300.

SMR

P39300. Positions 1-338.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12596N.

STRING

511145.b4192.

Proteomic databases

PRIDE

P39300.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77149; AAC77149; b4192.
BAE78193; BAE78193; BAE78193.

GeneID

12933718.
948705.

KEGG

ecj:Y75_p4078.
eco:b4192.

PATRIC

32123959. VBIEscCol129921_4324.

Organism-specific databases

EchoBASE

EB2385.

EcoGene

EG12492. ulaG.

Phylogenomic databases

eggNOG

COG2220.

HOGENOM

HOG000127385.

K03476.

OMA

RTVRMIP.

ProtClustDB

PRK11709.

Enzyme and pathway databases

BioCyc

EcoCyc:G7855-MONOMER.
ECOL316407:JW5868-MONOMER.
MetaCyc:G7855-MONOMER.

BRENDA

3.1.4.1. 2026.

UniPathway

UPA00263; UER00377.

Gene expression databases

Genevestigator

P39300.

Family and domain databases

HAMAP

MF_01266. UlaG.

InterPro

IPR001279. Beta-lactamas-like.
IPR023951. L-ascorbate_6P_UlaG.
[Graphical view]

SMART

SM00849. Lactamase_B. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P39300.

Entry information

Entry name

ULAG_ECOLI

Accession

Primary (citable) accession number: P39300
Secondary accession number(s): Q2M6B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents