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Protein

Epoxyqueuosine reductase

Gene

queG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).UniRule annotation

Catalytic activityi

Queuosine(34) in tRNA + acceptor + H2O = epoxyqueuosine(34) in tRNA + reduced acceptor.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi193Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi196Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi199Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi203Iron-sulfur 2UniRule annotation1
Metal bindingi246Iron-sulfur 2UniRule annotation1
Metal bindingi249Iron-sulfur 2UniRule annotation1
Metal bindingi253Iron-sulfur 1 (4Fe-4S)UniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • epoxyqueuosine reductase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • queuosine biosynthetic process Source: EcoCyc
  • tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7843-MONOMER.
ECOL316407:JW4124-MONOMER.
MetaCyc:G7843-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxyqueuosine reductaseUniRule annotation (EC:1.17.99.6UniRule annotation)
Alternative name(s):
Queuosine biosynthesis protein QueGUniRule annotation
Gene namesi
Name:queGUniRule annotation
Synonyms:yjeS
Ordered Locus Names:b4166, JW4124
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12481. queG.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNA from mutants accumulates epoxyqueuosine and lacks queuosine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001593041 – 379Epoxyqueuosine reductaseAdd BLAST379

Proteomic databases

PaxDbiP39288.
PRIDEiP39288.

Interactioni

Protein-protein interaction databases

BioGridi4262703. 4 interactors.
IntActiP39288. 2 interactors.
STRINGi511145.b4166.

Structurei

3D structure databases

ProteinModelPortaliP39288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini181 – 2134Fe-4S ferredoxin-typeUniRule annotationAdd BLAST33

Sequence similaritiesi

Belongs to the QueG family.UniRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EH2. Bacteria.
COG1600. LUCA.
HOGENOMiHOG000272643.
InParanoidiP39288.
KOiK18979.
OMAiICDTDLS.
PhylomeDBiP39288.

Family and domain databases

HAMAPiMF_00916. QueG. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR013542. DUF1730.
IPR009051. Helical_ferredxn.
IPR004453. QueG.
[Graphical view]
PfamiPF08331. DUF1730. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR00276. TIGR00276. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPLDLNQL AQKIKQWGLE LGFQQVGITD TDLSESEPKL QAWLDKQYHG
60 70 80 90 100
EMDWMARHGM LRARPHELLP GTLRVISVRM NYLPANAAFA STLKNPKLGY
110 120 130 140 150
VSRYALGRDY HKLLRNRLKK LGEMIQQHCV SLNFRPFVDS APILERPLAE
160 170 180 190 200
KAGLGWTGKH SLILNREAGS FFFLGELLVD IPLPVDQPVE EGCGKCVACM
210 220 230 240 250
TICPTGAIVE PYTVDARRCI SYLTIELEGA IPEELRPLMG NRIYGCDDCQ
260 270 280 290 300
LICPWNRYSQ LTTEEDFSPR KPLHAPELIE LFAWSEEKFL KVTEGSAIRR
310 320 330 340 350
IGHLRWLRNI AVALGNAPWD ETILTALESR KGEHPLLDEH IAWAIAQQIE
360 370
RRNACIVEVQ LPKKQRLVRV IEKGLPRDA
Length:379
Mass (Da):43,073
Last modified:February 1, 1995 - v1
Checksum:iE0440A59BCD753F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97062.1.
U00096 Genomic DNA. Translation: AAC77123.1.
AP009048 Genomic DNA. Translation: BAE78167.1.
PIRiS56391.
RefSeqiNP_418587.1. NC_000913.3.
WP_001294219.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77123; AAC77123; b4166.
BAE78167; BAE78167; BAE78167.
GeneIDi948686.
KEGGiecj:JW4124.
eco:b4166.
PATRICi32123905. VBIEscCol129921_4297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97062.1.
U00096 Genomic DNA. Translation: AAC77123.1.
AP009048 Genomic DNA. Translation: BAE78167.1.
PIRiS56391.
RefSeqiNP_418587.1. NC_000913.3.
WP_001294219.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262703. 4 interactors.
IntActiP39288. 2 interactors.
STRINGi511145.b4166.

Proteomic databases

PaxDbiP39288.
PRIDEiP39288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77123; AAC77123; b4166.
BAE78167; BAE78167; BAE78167.
GeneIDi948686.
KEGGiecj:JW4124.
eco:b4166.
PATRICi32123905. VBIEscCol129921_4297.

Organism-specific databases

EchoBASEiEB2374.
EcoGeneiEG12481. queG.

Phylogenomic databases

eggNOGiENOG4105EH2. Bacteria.
COG1600. LUCA.
HOGENOMiHOG000272643.
InParanoidiP39288.
KOiK18979.
OMAiICDTDLS.
PhylomeDBiP39288.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciEcoCyc:G7843-MONOMER.
ECOL316407:JW4124-MONOMER.
MetaCyc:G7843-MONOMER.

Miscellaneous databases

PROiP39288.

Family and domain databases

HAMAPiMF_00916. QueG. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR013542. DUF1730.
IPR009051. Helical_ferredxn.
IPR004453. QueG.
[Graphical view]
PfamiPF08331. DUF1730. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR00276. TIGR00276. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQUEG_ECOLI
AccessioniPrimary (citable) accession number: P39288
Secondary accession number(s): Q2M6D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.