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Protein

Putative ribosome biogenesis GTPase RsgA

Gene

rsgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96 fold increase in kcat) ribosomes (PubMed:14973029). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870).4 Publications

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi297 – 2971ZincBy similarity
Metal bindingi302 – 3021ZincBy similarity
Metal bindingi304 – 3041ZincBy similarity
Metal bindingi310 – 3101ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1634GTPBy similarity
Nucleotide bindingi214 – 2229GTPBy similarity

GO - Molecular functioni

  • GDP binding Source: EcoCyc
  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-HAMAP
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ribosomal small subunit biogenesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7841-MONOMER.
ECOL316407:JW4122-MONOMER.
MetaCyc:G7841-MONOMER.
SABIO-RKP39286.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ribosome biogenesis GTPase RsgA (EC:3.6.1.-)
Gene namesi
Name:rsgA
Synonyms:engC, yjeQ
Ordered Locus Names:b4161, JW4122
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12479. rsgA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate. The phenotype is partially suppressed by overexpression of a number of genes involved in ribosome function, including infB, era and ksgA.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi220 – 2201K → A: Reduction in GTPase activity. Strong reduction; when associated with A-221. 1 Publication
Mutagenesisi221 – 2211S → A: Reduction in GTPase activity. Strong reduction; when associated with A-220. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Putative ribosome biogenesis GTPase RsgAPRO_0000008149Add
BLAST

Proteomic databases

EPDiP39286.
PaxDbiP39286.
PRIDEiP39286.

Interactioni

Subunit structurei

Monomer (Probable). All of the protein is associated with ribosomes; the ratio is substoichiometric at 1:200 RsgA/ribosome. Association is tightest with the 30S subunit in the presence of the non-hydrolyzable GTP analog GMP-PNP.Curated

Protein-protein interaction databases

BioGridi4261080. 581 interactions.
DIPiDIP-12581N.
IntActiP39286. 32 interactions.
MINTiMINT-7290812.
STRINGi511145.b4161.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YKRelectron microscopy9.80W1-350[»]
4A2Ielectron microscopy16.50V35-337[»]
ProteinModelPortaliP39286.
SMRiP39286. Positions 35-337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39286.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 273170CP-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 113113Necessary for association with the ribosomeAdd
BLAST
Regioni1 – 2020Necessary for GMP-PNP-dependent association with the 30S ribosomal subunitAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi297 – 31014Knuckle-like cysteine clusterAdd
BLAST

Domaini

The knuckle-like cysteine cluster domain resembles the Zn-knuckle domain that is found in the nucleocapsid protein from HIV-1 virus, suggesting that it may bind RNA.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105E06. Bacteria.
COG1162. LUCA.
HOGENOMiHOG000006957.
InParanoidiP39286.
KOiK06949.
OMAiTHIIAAN.
PhylomeDBiP39286.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01820. GTPase_RsgA. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR027417. P-loop_NTPase.
IPR004881. Ribosome_biogen_GTPase_RsgA.
IPR010914. RsgA_GTPase_dom.
[Graphical view]
PfamiPF03193. DUF258. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00157. TIGR00157. 1 hit.
PROSITEiPS50936. ENGC_GTPASE. 1 hit.
PS51721. G_CP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNKLSKGQ QRRVNANHQR RLKTSKEKPD YDDNLFGEPD EGIVISRFGM
60 70 80 90 100
HADVESADGD VHRCNIRRTI RSLVTGDRVV WRPGKPAAEG VNVKGIVEAV
110 120 130 140 150
HERTSVLTRP DFYDGVKPIA ANIDQIVIVS AILPELSLNI IDRYLVACET
160 170 180 190 200
LQIEPIIVLN KIDLLDDEGM AFVNEQMDIY RNIGYRVLMV SSHTQDGLKP
210 220 230 240 250
LEEALTGRIS IFAGQSGVGK SSLLNALLGL QKEILTNDIS DNSGLGQHTT
260 270 280 290 300
TAARLYHFPH GGDVIDSPGV REFGLWHLEP EQITQGFVEF HDYLGLCKYR
310 320 330 340 350
DCKHDTDPGC AIREAVEEGK IAETRFENYH RILESMAQVK TRKNFSDTDD
Length:350
Mass (Da):39,193
Last modified:December 15, 1998 - v2
Checksum:iDEB14E36D2F0F378
GO

Sequence cautioni

The sequence AAA97060 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Mass spectrometryi

Molecular mass is 36830 Da from positions 21 - 350. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97060.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77121.2.
AP009048 Genomic DNA. Translation: BAE78165.1.
PIRiS56389.
RefSeqiNP_418585.4. NC_000913.3.
WP_000041964.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77121; AAC77121; b4161.
BAE78165; BAE78165; BAE78165.
GeneIDi948674.
KEGGiecj:JW4122.
eco:b4161.
PATRICi32123895. VBIEscCol129921_4295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97060.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77121.2.
AP009048 Genomic DNA. Translation: BAE78165.1.
PIRiS56389.
RefSeqiNP_418585.4. NC_000913.3.
WP_000041964.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YKRelectron microscopy9.80W1-350[»]
4A2Ielectron microscopy16.50V35-337[»]
ProteinModelPortaliP39286.
SMRiP39286. Positions 35-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261080. 581 interactions.
DIPiDIP-12581N.
IntActiP39286. 32 interactions.
MINTiMINT-7290812.
STRINGi511145.b4161.

Proteomic databases

EPDiP39286.
PaxDbiP39286.
PRIDEiP39286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77121; AAC77121; b4161.
BAE78165; BAE78165; BAE78165.
GeneIDi948674.
KEGGiecj:JW4122.
eco:b4161.
PATRICi32123895. VBIEscCol129921_4295.

Organism-specific databases

EchoBASEiEB2372.
EcoGeneiEG12479. rsgA.

Phylogenomic databases

eggNOGiENOG4105E06. Bacteria.
COG1162. LUCA.
HOGENOMiHOG000006957.
InParanoidiP39286.
KOiK06949.
OMAiTHIIAAN.
PhylomeDBiP39286.

Enzyme and pathway databases

BioCyciEcoCyc:G7841-MONOMER.
ECOL316407:JW4122-MONOMER.
MetaCyc:G7841-MONOMER.
SABIO-RKP39286.

Miscellaneous databases

EvolutionaryTraceiP39286.
PROiP39286.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01820. GTPase_RsgA. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR027417. P-loop_NTPase.
IPR004881. Ribosome_biogen_GTPase_RsgA.
IPR010914. RsgA_GTPase_dom.
[Graphical view]
PfamiPF03193. DUF258. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00157. TIGR00157. 1 hit.
PROSITEiPS50936. ENGC_GTPASE. 1 hit.
PS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSGA_ECOLI
AccessioniPrimary (citable) accession number: P39286
Secondary accession number(s): Q2M6E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was initially characterized with a propeptide of 20 residues; this is now thought to be the result of in vitro proteolysis when the protein is overexpressed.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.