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Protein

Miniconductance mechanosensitive channel MscM

Gene

mscM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed. Gates spontaneously in response to increased membrane tension.1 Publication

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7840-MONOMER

Protein family/group databases

TCDBi1.A.23.1.3 the small conductance mechanosensitive ion channel (mscs) family

Names & Taxonomyi

Protein namesi
Recommended name:
Miniconductance mechanosensitive channel MscM
Gene namesi
Name:mscM
Synonyms:yjeP
Ordered Locus Names:b4159, JW4120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12478 mscM

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Transmembranei522 – 542HelicalSequence analysisAdd BLAST21
Transmembranei551 – 571HelicalSequence analysisAdd BLAST21
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Transmembranei628 – 648HelicalSequence analysisAdd BLAST21
Transmembranei674 – 694HelicalSequence analysisAdd BLAST21
Transmembranei698 – 718HelicalSequence analysisAdd BLAST21
Transmembranei785 – 805HelicalSequence analysisAdd BLAST21
Transmembranei828 – 848HelicalSequence analysisAdd BLAST21
Transmembranei875 – 895HelicalSequence analysisAdd BLAST21
Transmembranei910 – 930HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003618020 – 1107Miniconductance mechanosensitive channel MscMAdd BLAST1088

Proteomic databases

PaxDbiP39285
PRIDEiP39285

Interactioni

Subunit structurei

Homoheptamer.1 Publication

Protein-protein interaction databases

BioGridi4262029, 305 interactors
IntActiP39285, 3 interactors
STRINGi316385.ECDH10B_4354

Structurei

3D structure databases

ProteinModelPortaliP39285
SMRiP39285
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGS Bacteria
COG3264 LUCA
HOGENOMiHOG000281473
KOiK22051
OMAiMALITFD
PhylomeDBiP39285

Family and domain databases

Gene3Di2.30.30.60, 1 hit
InterProiView protein in InterPro
IPR010920 LSM_dom_sf
IPR011066 MscC_channel_C
IPR006685 MscS_channel
IPR006686 MscS_channel_CS
IPR011014 MscS_channel_TM-2
IPR023408 MscS_dom_sf
IPR025692 MscS_IM_dom1
IPR024393 MscS_porin
PfamiView protein in Pfam
PF00924 MS_channel, 1 hit
PF12795 MscS_porin, 1 hit
PF12794 MscS_TM, 1 hit
SUPFAMiSSF50182 SSF50182, 1 hit
SSF82689 SSF82689, 1 hit
SSF82861 SSF82861, 1 hit
PROSITEiView protein in PROSITE
PS01246 UPF0003, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLIITFLMA WCLSWGAYAA TAPDSKQITQ ELEQAKAAKP AQPEVVEALQ
60 70 80 90 100
SALNALEERK GSLERIKQYQ QVIDNYPKLS ATLRAQLNNM RDEPRSVSPG
110 120 130 140 150
MSTDALNQEI LQVSSQLLDK SRQAQQEQER AREIADSLNQ LPQQQTDARR
160 170 180 190 200
QLNEIERRLG TLTGNTPLNQ AQNFALQSDS ARLKALVDEL ELAQLSANNR
210 220 230 240 250
QELARLRSEL AEKESQQLDA YLQALRNQLN SQRQLEAERA LESTELLAEN
260 270 280 290 300
SADLPKDIVA QFKINRELSA ALNQQAQRMD LVASQQRQAA SQTLQVRQAL
310 320 330 340 350
NTLREQSQWL GSSNLLGEAL RAQVARLPEM PKPQQLDTEM AQLRVQRLRY
360 370 380 390 400
EDLLNKQPLL RQIHQADGQP LTAEQNRILE AQLRTQRELL NSLLQGGDTL
410 420 430 440 450
LLELTKLKVS NGQLEDALKE VNEATHRYLF WTSDVRPMTI AWPLEIAQDL
460 470 480 490 500
RRLISLDTFS QLGKASVMML TSKETILPLF GALILVGCSI YSRRYFTRFL
510 520 530 540 550
ERSAAKVGKV TQDHFWLTLR TLFWSILVAS PLPVLWMTLG YGLREAWPYP
560 570 580 590 600
LAVAIGDGVT ATVPLLWVVM ICATFARPNG LFIAHFGWPR ERVSRGMRYY
610 620 630 640 650
LMSIGLIVPL IMALMMFDNL DDREFSGSLG RLCFILICGA LAVVTLSLKK
660 670 680 690 700
AGIPLYLNKE GSGDNITNHM LWNMMIGAPL VAILASAVGY LATAQALLAR
710 720 730 740 750
LETSVAIWFL LLVVYHVIRR WMLIQRRRLA FDRAKHRRAE MLAQRARGEE
760 770 780 790 800
EAHHHSSPEG AIEVDESEVD LDAISAQSLR LVRSILMLIA LLSVIVLWSE
810 820 830 840 850
IHSAFGFLEN ISLWDVTSTV QGVESLEPIT LGAVLIAILV FIITTQLVRN
860 870 880 890 900
LPALLELAIL QHLDLTPGTG YAITTITKYL LMLIGGLVGF SMIGIEWSKL
910 920 930 940 950
QWLVAALGVG LGFGLQEIFA NFISGLIILF EKPIRIGDTV TIRDLTGSVT
960 970 980 990 1000
KINTRATTIS DWDRKEIIVP NKAFITEQFI NWSLSDSVTR VVLTIPAPAD
1010 1020 1030 1040 1050
ANSEEVTEIL LTAARRCSLV IDNPAPEVFL VDLQQGIQIF ELRIYAAEMG
1060 1070 1080 1090 1100
HRMPLRHEIH QLILAGFHAH GIDMPFPPFQ MRLESLNGKQ TGRTLTSAGK

GRQAGSL
Length:1,107
Mass (Da):123,968
Last modified:July 15, 1998 - v3
Checksum:i5F52A2993B90532B
GO

Sequence cautioni

The sequence AAA83897 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1015R → A in AAA97058 (PubMed:7610040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97058.1
U00096 Genomic DNA Translation: AAC77119.1
AP009048 Genomic DNA Translation: BAE78163.1
J03916 Genomic DNA Translation: AAA83897.1 Different initiation.
PIRiE65226
RefSeqiNP_418583.1, NC_000913.3
WP_001236847.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77119; AAC77119; b4159
BAE78163; BAE78163; BAE78163
GeneIDi948676
KEGGiecj:JW4120
eco:b4159
PATRICifig|1411691.4.peg.2539

Similar proteinsi

Entry informationi

Entry nameiMSCM_ECOLI
AccessioniPrimary (citable) accession number: P39285
Secondary accession number(s): P76798, Q2M6E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health