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Protein

Miniconductance mechanosensitive channel MscM

Gene

mscM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed. Gates spontaneously in response to increased membrane tension.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7840-MONOMER.
ECOL316407:JW4120-MONOMER.

Protein family/group databases

TCDBi1.A.23.1.3. the small conductance mechanosensitive ion channel (mscs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Miniconductance mechanosensitive channel MscM
Gene namesi
Name:mscM
Synonyms:yjeP
Ordered Locus Names:b4159, JW4120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12478. mscM.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei467 – 48721HelicalSequence analysisAdd
BLAST
Transmembranei522 – 54221HelicalSequence analysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence analysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence analysisAdd
BLAST
Transmembranei628 – 64821HelicalSequence analysisAdd
BLAST
Transmembranei674 – 69421HelicalSequence analysisAdd
BLAST
Transmembranei698 – 71821HelicalSequence analysisAdd
BLAST
Transmembranei785 – 80521HelicalSequence analysisAdd
BLAST
Transmembranei828 – 84821HelicalSequence analysisAdd
BLAST
Transmembranei875 – 89521HelicalSequence analysisAdd
BLAST
Transmembranei910 – 93021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 11071088Miniconductance mechanosensitive channel MscMPRO_0000036180Add
BLAST

Proteomic databases

PaxDbiP39285.
PRIDEiP39285.

Interactioni

Subunit structurei

Homoheptamer.1 Publication

Protein-protein interaction databases

BioGridi4262029. 299 interactions.
IntActiP39285. 3 interactions.
STRINGi511145.b4159.

Structurei

3D structure databases

ProteinModelPortaliP39285.
SMRiP39285. Positions 829-1077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000281473.
KOiK05802.
OMAiARAMRYY.
OrthoDBiEOG6J1DC5.
PhylomeDBiP39285.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLIITFLMA WCLSWGAYAA TAPDSKQITQ ELEQAKAAKP AQPEVVEALQ
60 70 80 90 100
SALNALEERK GSLERIKQYQ QVIDNYPKLS ATLRAQLNNM RDEPRSVSPG
110 120 130 140 150
MSTDALNQEI LQVSSQLLDK SRQAQQEQER AREIADSLNQ LPQQQTDARR
160 170 180 190 200
QLNEIERRLG TLTGNTPLNQ AQNFALQSDS ARLKALVDEL ELAQLSANNR
210 220 230 240 250
QELARLRSEL AEKESQQLDA YLQALRNQLN SQRQLEAERA LESTELLAEN
260 270 280 290 300
SADLPKDIVA QFKINRELSA ALNQQAQRMD LVASQQRQAA SQTLQVRQAL
310 320 330 340 350
NTLREQSQWL GSSNLLGEAL RAQVARLPEM PKPQQLDTEM AQLRVQRLRY
360 370 380 390 400
EDLLNKQPLL RQIHQADGQP LTAEQNRILE AQLRTQRELL NSLLQGGDTL
410 420 430 440 450
LLELTKLKVS NGQLEDALKE VNEATHRYLF WTSDVRPMTI AWPLEIAQDL
460 470 480 490 500
RRLISLDTFS QLGKASVMML TSKETILPLF GALILVGCSI YSRRYFTRFL
510 520 530 540 550
ERSAAKVGKV TQDHFWLTLR TLFWSILVAS PLPVLWMTLG YGLREAWPYP
560 570 580 590 600
LAVAIGDGVT ATVPLLWVVM ICATFARPNG LFIAHFGWPR ERVSRGMRYY
610 620 630 640 650
LMSIGLIVPL IMALMMFDNL DDREFSGSLG RLCFILICGA LAVVTLSLKK
660 670 680 690 700
AGIPLYLNKE GSGDNITNHM LWNMMIGAPL VAILASAVGY LATAQALLAR
710 720 730 740 750
LETSVAIWFL LLVVYHVIRR WMLIQRRRLA FDRAKHRRAE MLAQRARGEE
760 770 780 790 800
EAHHHSSPEG AIEVDESEVD LDAISAQSLR LVRSILMLIA LLSVIVLWSE
810 820 830 840 850
IHSAFGFLEN ISLWDVTSTV QGVESLEPIT LGAVLIAILV FIITTQLVRN
860 870 880 890 900
LPALLELAIL QHLDLTPGTG YAITTITKYL LMLIGGLVGF SMIGIEWSKL
910 920 930 940 950
QWLVAALGVG LGFGLQEIFA NFISGLIILF EKPIRIGDTV TIRDLTGSVT
960 970 980 990 1000
KINTRATTIS DWDRKEIIVP NKAFITEQFI NWSLSDSVTR VVLTIPAPAD
1010 1020 1030 1040 1050
ANSEEVTEIL LTAARRCSLV IDNPAPEVFL VDLQQGIQIF ELRIYAAEMG
1060 1070 1080 1090 1100
HRMPLRHEIH QLILAGFHAH GIDMPFPPFQ MRLESLNGKQ TGRTLTSAGK

GRQAGSL
Length:1,107
Mass (Da):123,968
Last modified:July 15, 1998 - v3
Checksum:i5F52A2993B90532B
GO

Sequence cautioni

The sequence AAA83897.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1015 – 10151R → A in AAA97058 (PubMed:7610040).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97058.1.
U00096 Genomic DNA. Translation: AAC77119.1.
AP009048 Genomic DNA. Translation: BAE78163.1.
J03916 Genomic DNA. Translation: AAA83897.1. Different initiation.
PIRiE65226.
RefSeqiNP_418583.1. NC_000913.3.
WP_001236847.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77119; AAC77119; b4159.
BAE78163; BAE78163; BAE78163.
GeneIDi948676.
KEGGiecj:JW4120.
eco:b4159.
PATRICi32123891. VBIEscCol129921_4293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97058.1.
U00096 Genomic DNA. Translation: AAC77119.1.
AP009048 Genomic DNA. Translation: BAE78163.1.
J03916 Genomic DNA. Translation: AAA83897.1. Different initiation.
PIRiE65226.
RefSeqiNP_418583.1. NC_000913.3.
WP_001236847.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39285.
SMRiP39285. Positions 829-1077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262029. 299 interactions.
IntActiP39285. 3 interactions.
STRINGi511145.b4159.

Protein family/group databases

TCDBi1.A.23.1.3. the small conductance mechanosensitive ion channel (mscs) family.

Proteomic databases

PaxDbiP39285.
PRIDEiP39285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77119; AAC77119; b4159.
BAE78163; BAE78163; BAE78163.
GeneIDi948676.
KEGGiecj:JW4120.
eco:b4159.
PATRICi32123891. VBIEscCol129921_4293.

Organism-specific databases

EchoBASEiEB2371.
EcoGeneiEG12478. mscM.

Phylogenomic databases

eggNOGiENOG4105EGS. Bacteria.
COG3264. LUCA.
HOGENOMiHOG000281473.
KOiK05802.
OMAiARAMRYY.
OrthoDBiEOG6J1DC5.
PhylomeDBiP39285.

Enzyme and pathway databases

BioCyciEcoCyc:G7840-MONOMER.
ECOL316407:JW4120-MONOMER.

Miscellaneous databases

PROiP39285.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR006686. MscS_channel_CS.
IPR011014. MscS_channel_TM-2.
IPR025692. MscS_IM_dom1.
IPR024393. MscS_porin.
[Graphical view]
PfamiPF00924. MS_channel. 1 hit.
PF12795. MscS_porin. 1 hit.
PF12794. MscS_TM. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
SSF82861. SSF82861. 1 hit.
PROSITEiPS01246. UPF0003. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 1015.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Structural characterization of Escherichia coli phosphatidylserine decarboxylase."
    Li Q.-X., Dowhan W.
    J. Biol. Chem. 263:11516-11522(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    Strain: K12.
  5. "Characterization of three novel mechanosensitive channel activities in Escherichia coli."
    Edwards M.D., Black S., Rasmussen T., Rasmussen A., Stokes N.R., Stephen T.L., Miller S., Booth I.R.
    Channels 6:272-281(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, GENE NAME.

Entry informationi

Entry nameiMSCM_ECOLI
AccessioniPrimary (citable) accession number: P39285
Secondary accession number(s): P76798, Q2M6E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: February 17, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.