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Protein

L-methionine/branched-chain amino acid exporter YjeH

Gene

yjeH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the efflux of L-methionine. Can also export L-leucine, L-isoleucine and L-valine. Activity is dependent on electrochemical potential.1 Publication

Enzyme regulationi

Efflux is inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: EcoCyc
  • isoleucine transmembrane transport Source: EcoCyc
  • leucine transport Source: EcoCyc
  • L-valine transmembrane transport Source: EcoCyc
  • methionine transport Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B4141-MONOMER
MetaCyc:B4141-MONOMER

Protein family/group databases

TCDBi2.A.3.13.1 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
L-methionine/branched-chain amino acid exporter YjeHCurated
Gene namesi
Name:yjeH
Ordered Locus Names:b4141, JW4101
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12470 yjeH

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15PeriplasmicCuratedAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 41CytoplasmicCurated5
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 89PeriplasmicCuratedAdd BLAST27
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 113CytoplasmicCurated3
Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
Topological domaini135 – 147PeriplasmicCuratedAdd BLAST13
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 182CytoplasmicCuratedAdd BLAST14
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 219PeriplasmicCuratedAdd BLAST16
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 257CytoplasmicCuratedAdd BLAST17
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279 – 317PeriplasmicCuratedAdd BLAST39
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 341CytoplasmicCurated3
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 378PeriplasmicCuratedAdd BLAST16
Transmembranei379 – 399HelicalSequence analysisAdd BLAST21
Topological domaini400 – 418Cytoplasmic1 PublicationAdd BLAST19

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion increases the susceptibility to DL-ethionine, DL-norleucine and DL-norvaline. Deletion also results in intracellular accumulation and a reduced rate of export of methionine in the presence of extracellular Met-Met dipeptides.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24T → Y: Strong decrease in methionine efflux. 1 Publication1
Mutagenesisi25G → F: Strong decrease in methionine efflux. 1 Publication1
Mutagenesisi195W → A: Strong decrease in methionine efflux. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001697381 – 418L-methionine/branched-chain amino acid exporter YjeHAdd BLAST418

Proteomic databases

PaxDbiP39277
PRIDEiP39277

Expressioni

Inductioni

Induced in the presence of intracellular L-methionine, L-leucine or L-isoleucine.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262197, 12 interactors
DIPiDIP-12573N
IntActiP39277, 4 interactors
STRINGi316385.ECDH10B_4334

Structurei

3D structure databases

ProteinModelPortaliP39277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DVH Bacteria
COG0531 LUCA
HOGENOMiHOG000273866
InParanoidiP39277
KOiK16263
OMAiHMGEEFK
PhylomeDBiP39277

Family and domain databases

InterProiView protein in InterPro
IPR002293 AA/rel_permease1
PfamiView protein in Pfam
PF13520 AA_permease_2, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit

Sequencei

Sequence statusi: Complete.

P39277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLKQELGL AQGIGLLSTS LLGTGVFAVP ALAALVAGNN SLWAWPVLII
60 70 80 90 100
LVFPIAIVFA ILGRHYPSAG GVAHFVGMAF GSRLERVTGW LFLSVIPVGL
110 120 130 140 150
PAALQIAAGF GQAMFGWHSW QLLLAELGTL ALVWYIGTRG ASSSANLQTV
160 170 180 190 200
IAGLIVALIV AIWWAGDIKP ANIPFPAPGN IELTGLFAAL SVMFWCFVGL
210 220 230 240 250
EAFAHLASEF KNPERDFPRA LMIGLLLAGL VYWGCTVVVL HFDAYGEKMA
260 270 280 290 300
AAASLPKIVV QLFGVGALWI ACVIGYLACF ASLNIYIQSF ARLVWSQAQH
310 320 330 340 350
NPDHYLARLS SRHIPNNALN AVLGCCVVST LVIHALEINL DALIIYANGI
360 370 380 390 400
FIMIYLLCML AGCKLLQGRY RLLAVVGGLL CVLLLAMVGW KSLYALIMLA
410
GLWLLLPKRK TPENGITT
Length:418
Mass (Da):44,778
Last modified:February 1, 1995 - v1
Checksum:i9244E8281BC54AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97040.1
U00096 Genomic DNA Translation: AAC77101.1
AP009048 Genomic DNA Translation: BAE78143.1
PIRiS56369
RefSeqiNP_418565.1, NC_000913.3
WP_000015837.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77101; AAC77101; b4141
BAE78143; BAE78143; BAE78143
GeneIDi948656
KEGGiecj:JW4101
eco:b4141
PATRICifig|1411691.4.peg.2559

Similar proteinsi

Entry informationi

Entry nameiYJEH_ECOLI
AccessioniPrimary (citable) accession number: P39277
Secondary accession number(s): Q2M6G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health