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Protein

L-methionine/branched-chain amino acid exporter YjeH

Gene

yjeH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the efflux of L-methionine. Can also export L-leucine, L-isoleucine and L-valine. Activity is dependent on electrochemical potential.1 Publication

Enzyme regulationi

Efflux is inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to amino acid stimulus Source: EcoCyc
  • isoleucine transmembrane transport Source: EcoCyc
  • leucine transport Source: EcoCyc
  • L-valine transmembrane transport Source: EcoCyc
  • methionine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B4141-MONOMER.
ECOL316407:JW4101-MONOMER.

Protein family/group databases

TCDBi2.A.3.13.1. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
L-methionine/branched-chain amino acid exporter YjeHCurated
Gene namesi
Name:yjeH
Ordered Locus Names:b4141, JW4101
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12470. yjeH.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515PeriplasmicCuratedAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 415CytoplasmicCurated
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Topological domaini63 – 8927PeriplasmicCuratedAdd
BLAST
Transmembranei90 – 11021HelicalSequence analysisAdd
BLAST
Topological domaini111 – 1133CytoplasmicCurated
Transmembranei114 – 13421HelicalSequence analysisAdd
BLAST
Topological domaini135 – 14713PeriplasmicCuratedAdd
BLAST
Transmembranei148 – 16821HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18214CytoplasmicCuratedAdd
BLAST
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST
Topological domaini204 – 21916PeriplasmicCuratedAdd
BLAST
Transmembranei220 – 24021HelicalSequence analysisAdd
BLAST
Topological domaini241 – 25717CytoplasmicCuratedAdd
BLAST
Transmembranei258 – 27821HelicalSequence analysisAdd
BLAST
Topological domaini279 – 31739PeriplasmicCuratedAdd
BLAST
Transmembranei318 – 33821HelicalSequence analysisAdd
BLAST
Topological domaini339 – 3413CytoplasmicCurated
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 37816PeriplasmicCuratedAdd
BLAST
Transmembranei379 – 39921HelicalSequence analysisAdd
BLAST
Topological domaini400 – 41819Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion increases the susceptibility to DL-ethionine, DL-norleucine and DL-norvaline. Deletion also results in intracellular accumulation and a reduced rate of export of methionine in the presence of extracellular Met-Met dipeptides.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241T → Y: Strong decrease in methionine efflux. 1 Publication
Mutagenesisi25 – 251G → F: Strong decrease in methionine efflux. 1 Publication
Mutagenesisi195 – 1951W → A: Strong decrease in methionine efflux. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418L-methionine/branched-chain amino acid exporter YjeHPRO_0000169738Add
BLAST

Proteomic databases

PaxDbiP39277.

Expressioni

Inductioni

Induced in the presence of intracellular L-methionine, L-leucine or L-isoleucine.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262197. 12 interactions.
DIPiDIP-12573N.
IntActiP39277. 3 interactions.
STRINGi511145.b4141.

Structurei

3D structure databases

ProteinModelPortaliP39277.
SMRiP39277. Positions 6-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DVH. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000273866.
InParanoidiP39277.
KOiK16263.
OMAiTVYWACT.
PhylomeDBiP39277.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P39277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLKQELGL AQGIGLLSTS LLGTGVFAVP ALAALVAGNN SLWAWPVLII
60 70 80 90 100
LVFPIAIVFA ILGRHYPSAG GVAHFVGMAF GSRLERVTGW LFLSVIPVGL
110 120 130 140 150
PAALQIAAGF GQAMFGWHSW QLLLAELGTL ALVWYIGTRG ASSSANLQTV
160 170 180 190 200
IAGLIVALIV AIWWAGDIKP ANIPFPAPGN IELTGLFAAL SVMFWCFVGL
210 220 230 240 250
EAFAHLASEF KNPERDFPRA LMIGLLLAGL VYWGCTVVVL HFDAYGEKMA
260 270 280 290 300
AAASLPKIVV QLFGVGALWI ACVIGYLACF ASLNIYIQSF ARLVWSQAQH
310 320 330 340 350
NPDHYLARLS SRHIPNNALN AVLGCCVVST LVIHALEINL DALIIYANGI
360 370 380 390 400
FIMIYLLCML AGCKLLQGRY RLLAVVGGLL CVLLLAMVGW KSLYALIMLA
410
GLWLLLPKRK TPENGITT
Length:418
Mass (Da):44,778
Last modified:February 1, 1995 - v1
Checksum:i9244E8281BC54AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97040.1.
U00096 Genomic DNA. Translation: AAC77101.1.
AP009048 Genomic DNA. Translation: BAE78143.1.
PIRiS56369.
RefSeqiNP_418565.1. NC_000913.3.
WP_000015837.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77101; AAC77101; b4141.
BAE78143; BAE78143; BAE78143.
GeneIDi948656.
KEGGiecj:JW4101.
eco:b4141.
PATRICi32123851. VBIEscCol129921_4273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97040.1.
U00096 Genomic DNA. Translation: AAC77101.1.
AP009048 Genomic DNA. Translation: BAE78143.1.
PIRiS56369.
RefSeqiNP_418565.1. NC_000913.3.
WP_000015837.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39277.
SMRiP39277. Positions 6-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262197. 12 interactions.
DIPiDIP-12573N.
IntActiP39277. 3 interactions.
STRINGi511145.b4141.

Protein family/group databases

TCDBi2.A.3.13.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP39277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77101; AAC77101; b4141.
BAE78143; BAE78143; BAE78143.
GeneIDi948656.
KEGGiecj:JW4101.
eco:b4141.
PATRICi32123851. VBIEscCol129921_4273.

Organism-specific databases

EchoBASEiEB2363.
EcoGeneiEG12470. yjeH.

Phylogenomic databases

eggNOGiENOG4105DVH. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000273866.
InParanoidiP39277.
KOiK16263.
OMAiTVYWACT.
PhylomeDBiP39277.

Enzyme and pathway databases

BioCyciEcoCyc:B4141-MONOMER.
ECOL316407:JW4101-MONOMER.

Miscellaneous databases

PROiP39277.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYJEH_ECOLI
AccessioniPrimary (citable) accession number: P39277
Secondary accession number(s): Q2M6G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.