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Protein

Dipeptide and tripeptide permease C

Gene

dtpC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-dependent permease that transports di- and tripeptides. Shows significantly higher specificity towards dipeptides than tripeptides. Has a preference for dipeptides with a C-terminal Lys residue. Can bind Ala-Lys, Lys-Ala, Ala-Ala. Can also transport alanine and trialanine.4 Publications

Kineticsi

  1. KM=0.07 mM for Ala-Lys1 Publication
  2. KM=0.51 mM for Lys-Ala1 Publication

    pH dependencei

    Optimum pH is around 6.5.1 Publication

    GO - Molecular functioni

    • dipeptide transmembrane transporter activity Source: EcoCyc
    • hydrogen ion transmembrane transporter activity Source: EcoCyc
    • peptide:proton symporter activity Source: EcoCyc
    • tripeptide transporter activity Source: EcoCyc

    GO - Biological processi

    • dipeptide transmembrane transport Source: EcoCyc
    • hydrogen ion transmembrane transport Source: EcoCyc
    • protein transport Source: UniProtKB-KW
    • tripeptide transmembrane transport Source: EcoCyc
    Complete GO annotation...

    Keywords - Biological processi

    Peptide transport, Protein transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:YJDL-MONOMER.
    ECOL316407:JW4091-MONOMER.
    MetaCyc:YJDL-MONOMER.
    BRENDAi3.6.3.43. 2026.

    Protein family/group databases

    TCDBi2.A.17.1.5. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptide and tripeptide permease CCurated
    Alternative name(s):
    Dipeptide/tripeptide:H(+) symporter DtpCCurated
    Gene namesi
    Name:dtpC1 Publication
    Synonyms:yjdL
    Ordered Locus Names:b4130, JW4091
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12469. dtpC.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei13 – 3321HelicalSequence analysisAdd
    BLAST
    Topological domaini34 – 4613PeriplasmicSequence analysisAdd
    BLAST
    Transmembranei47 – 6721HelicalSequence analysisAdd
    BLAST
    Topological domaini68 – 703CytoplasmicSequence analysis
    Transmembranei71 – 9323HelicalSequence analysisAdd
    BLAST
    Topological domaini94 – 1029PeriplasmicSequence analysis
    Transmembranei103 – 12523HelicalSequence analysisAdd
    BLAST
    Topological domaini126 – 14015CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence analysisAdd
    BLAST
    Topological domaini162 – 1643PeriplasmicSequence analysis
    Transmembranei165 – 18521HelicalSequence analysisAdd
    BLAST
    Topological domaini186 – 20823CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence analysisAdd
    BLAST
    Topological domaini230 – 2345PeriplasmicSequence analysis
    Transmembranei235 – 25521HelicalSequence analysisAdd
    BLAST
    Topological domaini256 – 2627CytoplasmicSequence analysis
    Transmembranei263 – 28321HelicalSequence analysisAdd
    BLAST
    Topological domaini284 – 30724PeriplasmicSequence analysisAdd
    BLAST
    Transmembranei308 – 32821HelicalSequence analysisAdd
    BLAST
    Topological domaini329 – 34012CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei341 – 36121HelicalSequence analysisAdd
    BLAST
    Topological domaini362 – 37514PeriplasmicSequence analysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence analysisAdd
    BLAST
    Topological domaini397 – 40610CytoplasmicSequence analysis
    Transmembranei407 – 42721HelicalSequence analysisAdd
    BLAST
    Topological domaini428 – 44619PeriplasmicSequence analysisAdd
    BLAST
    Transmembranei447 – 46721HelicalSequence analysisAdd
    BLAST
    Topological domaini468 – 48518Cytoplasmic1 PublicationAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi20 – 201E → A: Lack of activity. 1 Publication
    Mutagenesisi20 – 201E → D: Decrease in activity. 1 Publication
    Mutagenesisi20 – 201E → Q: Decrease in activity. Abolishes the pH dependency. 1 Publication
    Mutagenesisi388 – 3881E → A: Lack of activity. 1 Publication
    Mutagenesisi388 – 3881E → D: Decrease in activity. 1 Publication
    Mutagenesisi388 – 3881E → Q: Decrease in activity. Does not bind Ala-Lys dipeptide. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Dipeptide and tripeptide permease CPRO_0000064330Add
    BLAST

    Proteomic databases

    PaxDbiP39276.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi4261129. 143 interactions.
    STRINGi511145.b4130.

    Structurei

    3D structure databases

    ProteinModelPortaliP39276.
    SMRiP39276. Positions 5-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105DUX. Bacteria.
    COG3104. LUCA.
    HOGENOMiHOG000243450.
    InParanoidiP39276.
    KOiK03305.
    OMAiISYLIMT.
    OrthoDBiEOG6QP0WR.
    PhylomeDBiP39276.

    Family and domain databases

    InterProiIPR005279. Dipep/tripep_permease.
    IPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00924. yjdL_sub1_fam. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39276-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTPSQPRAI YYIVAIQIWE YFSFYGMRAL LILYLTHQLG FDDNHAISLF
    60 70 80 90 100
    SAYASLVYVT PILGGWLADR LLGNRTAVIA GALLMTLGHV VLGIDTNSTF
    110 120 130 140 150
    SLYLALAIII CGYGLFKSNI SCLLGELYDE NDHRRDGGFS LLYAAGNIGS
    160 170 180 190 200
    IAAPIACGLA AQWYGWHVGF ALAGGGMFIG LLIFLSGHRH FQSTRSMDKK
    210 220 230 240 250
    ALTSVKFALP VWSWLVVMLC LAPVFFTLLL ENDWSGYLLA IVCLIAAQII
    260 270 280 290 300
    ARMMIKFPEH RRALWQIVLL MFVGTLFWVL AQQGGSTISL FIDRFVNRQA
    310 320 330 340 350
    FNIEVPTALF QSVNAIAVML AGVVLAWLAS PESRGNSTLR VWLKFAFGLL
    360 370 380 390 400
    LMACGFMLLA FDARHAAADG QASMGVMISG LALMGFAELF IDPVAIAQIT
    410 420 430 440 450
    RLKMSGVLTG IYMLATGAVA NWLAGVVAQQ TTESQISGMA IAAYQRFFSQ
    460 470 480
    MGEWTLACVA IIVVLAFATR FLFSTPTNMI QESND
    Length:485
    Mass (Da):53,055
    Last modified:February 1, 1995 - v1
    Checksum:iACBE0B947DE0CB61
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97030.1.
    U00096 Genomic DNA. Translation: AAC77091.1.
    AP009048 Genomic DNA. Translation: BAE78133.1.
    PIRiS56359.
    RefSeqiNP_418554.1. NC_000913.3.
    WP_000856829.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77091; AAC77091; b4130.
    BAE78133; BAE78133; BAE78133.
    GeneIDi948644.
    KEGGiecj:JW4091.
    eco:b4130.
    PATRICi32123827. VBIEscCol129921_4262.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA. Translation: AAA97030.1.
    U00096 Genomic DNA. Translation: AAC77091.1.
    AP009048 Genomic DNA. Translation: BAE78133.1.
    PIRiS56359.
    RefSeqiNP_418554.1. NC_000913.3.
    WP_000856829.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP39276.
    SMRiP39276. Positions 5-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261129. 143 interactions.
    STRINGi511145.b4130.

    Protein family/group databases

    TCDBi2.A.17.1.5. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiP39276.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77091; AAC77091; b4130.
    BAE78133; BAE78133; BAE78133.
    GeneIDi948644.
    KEGGiecj:JW4091.
    eco:b4130.
    PATRICi32123827. VBIEscCol129921_4262.

    Organism-specific databases

    EchoBASEiEB2362.
    EcoGeneiEG12469. dtpC.

    Phylogenomic databases

    eggNOGiENOG4105DUX. Bacteria.
    COG3104. LUCA.
    HOGENOMiHOG000243450.
    InParanoidiP39276.
    KOiK03305.
    OMAiISYLIMT.
    OrthoDBiEOG6QP0WR.
    PhylomeDBiP39276.

    Enzyme and pathway databases

    BioCyciEcoCyc:YJDL-MONOMER.
    ECOL316407:JW4091-MONOMER.
    MetaCyc:YJDL-MONOMER.
    BRENDAi3.6.3.43. 2026.

    Miscellaneous databases

    PROiP39276.

    Family and domain databases

    InterProiIPR005279. Dipep/tripep_permease.
    IPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00924. yjdL_sub1_fam. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Ligand binding analyses of the putative peptide transporter YjdL from E. coli display a significant selectivity towards dipeptides."
      Ernst H.A., Pham A., Hald H., Kastrup J.S., Rahman M., Mirza O.
      Biochem. Biophys. Res. Commun. 389:112-116(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Projection structure of DtpD (YbgH), a prokaryotic member of the peptide transporter family."
      Casagrande F., Harder D., Schenk A., Meury M., Ucurum Z., Engel A., Weitz D., Daniel H., Fotiadis D.
      J. Mol. Biol. 394:708-717(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME.
    7. "Biophysical characterization of the proton-coupled oligopeptide transporter YjdL."
      Jensen J.M., Simonsen F.C., Mastali A., Hald H., Lillebro I., Diness F., Olsen L., Mirza O.
      Peptides 38:89-93(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLU-388.
    8. "Functional investigation of conserved membrane-embedded glutamate residues in the proton-coupled peptide transporter YjdL."
      Jensen J.M., Ernst H., Wang X., Hald H., Ditta A.C., Ismat F., Rahman M., Mirza O.
      Protein Pept. Lett. 19:282-287(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-20 AND GLU-388.
    9. "New insights into the substrate specificities of proton-coupled oligopeptide transporters from E. coli by a pH sensitive assay."
      Prabhala B.K., Aduri N.G., Jensen J.M., Ernst H.A., Iram N., Rahman M., Mirza O.
      FEBS Lett. 588:560-565(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiDTPC_ECOLI
    AccessioniPrimary (citable) accession number: P39276
    Secondary accession number(s): Q2M6H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: January 20, 2016
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.