P39265 (ALSB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-allose-binding periplasmic protein
Short name=ALBP

Gene names

Name:	alsB
Synonyms:	yjcX
Ordered Locus Names:	b4088, JW4049

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system AlsBAC for D-allose. Ref.5
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 2 family.

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Periplasm
Domain	Signal
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from direct assay. Source: UniProtKB periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Chain

24 – 311

288

D-allose-binding periplasmic protein

PRO_0000031719

Experimental info

Sequence conflict

16

1

M → I in CAA57686. Ref.4

Sequence conflict

125 – 130

6

AGGNVE → LAQCGS in CAA57686. Ref.4

Secondary structure

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311

Helix Strand Turn

Details...

Beta strand

26 – 32

7

Beta strand

34 – 36

3

Helix

37 – 53

17

Beta strand

57 – 61

5

Helix

68 – 79

12

Beta strand

81 – 88

8

Beta strand

91 – 94

4

Helix

97 – 105

9

Beta strand

109 – 115

7

Helix

119 – 124

6

Beta strand

130 – 134

5

Helix

137 – 152

16

Helix

153 – 155

3

Beta strand

157 – 163

7

Helix

169 – 183

15

Beta strand

188 – 195

8

Helix

200 – 213

14

Beta strand

219 – 224

6

Helix

225 – 237

13

Turn

241 – 243

3

Beta strand

244 – 250

7

Helix

253 – 260

8

Beta strand

266 – 269

4

Helix

272 – 288

17

Beta strand

300 – 303

4

Beta strand

307 – 309

3

Sequences

	Sequence		Length	Mass (Da)
	P39265 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1A60E05BD1846068	FASTA	311	32,910
	10 20 30 40 50 60 MNKYLKYFSG TLVGLMLSTS AFAAAEYAVV LKTLSNPFWV DMKKGIEDEA KTLGVSVDIF 70 80 90 100 110 120 ASPSEGDFQS QLQLFEDLSN KNYKGIAFAP LSSVNLVMPV ARAWKKGIYL VNLDEKIDMD 130 140 150 160 170 180 NLKKAGGNVE AFVTTDNVAV GAKGASFIID KLGAEGGEVA IIEGKAGNAS GEARRNGATE 190 200 210 220 230 240 AFKKASQIKL VASQPADWDR IKALDVATNV LQRNPNIKAI YCANDTMAMG VAQAVANAGK 250 260 270 280 290 300 TGKVLVVGTD GIPEARKMVE AGQMTATVAQ NPADIGATGL KLMVDAEKSG KVIPLDKAPE 310 FKLVDSILVT Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression." Soerensen K.I., Hove-Jensen B. J. Bacteriol. 178:1003-1011(1996) [PubMed: 8576032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130. Strain: K12.
[5]	"The D-allose operon of Escherichia coli K-12." Kim C., Song S., Park C. J. Bacteriol. 179:7631-7637(1997) [PubMed: 9401019] [Abstract] Cited for: FUNCTION.
[6]	"Structure of D-allose binding protein from Escherichia coli bound to D-allose at 1.8-A resolution." Chaudhuri B.N., Ko J., Park C., Jones T.A., Mowbray S.L. J. Mol. Biol. 286:1519-1531(1999) [PubMed: 10064713] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U14003 Genomic DNA. Translation: AAA96987.1.
U00096 Genomic DNA. Translation: AAC77049.1.
AP009048 Genomic DNA. Translation: BAE78091.1.
X82203 Genomic DNA. Translation: CAA57686.1.

PIR

S56316.

RefSeq

NP_418512.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GUB	X-ray	3.10	A	24-311	[»]
1GUD	X-ray	1.70	A/B	24-311	[»]
1RPJ	X-ray	1.80	A	24-311	[»]

ProteinModelPortal

P39265.

SMR

P39265. Positions 24-311.

ModBase

Search...

Protein-protein interaction databases

IntAct

P39265. 4 interactions.

Protein family/group databases

TCDB

3.A.1.2.6. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000526; EBESCP00000000526; EBESCG00000000444.
EBESCT00000000527; EBESCP00000000527; EBESCG00000000444.
EBESCT00000014898; EBESCP00000014189; EBESCG00000013958.

GeneID

948604.

GenomeReviews

Gene locus JW4049 in contig AP009048_GR.
Gene locus b4088 in contig U00096_GR.

KEGG

ecj:JW4049.
eco:b4088.

PATRIC

32123733. VBIEscCol129921_4215.

Organism-specific databases

EchoBASE

EB2352.

EcoGene

EG12458. alsB.

Phylogenomic databases

eggNOG

COG1879.

GeneTree

EBGT00050000009242.

HOGENOM

HBG617058.

OMA

QMTATIA.

PhylomeDB

P39265.

ProtClustDB

PRK09701.

Enzyme and pathway databases

BioCyc

EcoCyc:YJCX-MONOMER.

Gene expression databases

Genevestigator

P39265.

Family and domain databases

InterPro

IPR001761. Peripla_BP_Lac1.
[Graphical view]

KO

K10549.

Pfam

PF00532. Peripla_BP_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALSB_ECOLI

Accession

Primary (citable) accession number: P39265
Secondary accession number(s): Q2M6L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program