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P39217 (TLPB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-accepting chemotaxis protein TlpB
Gene names
Name:tlpB
Ordered Locus Names:BSU31230
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length662 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Sequence similarities

Contains 1 cache domain.

Contains 1 HAMP domain.

Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionTransducer
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 662662Methyl-accepting chemotaxis protein TlpB
PRO_0000110560

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721Helical; Potential
Topological domain38 – 281244Extracellular Potential
Transmembrane282 – 30221Helical; Potential
Topological domain303 – 662360Cytoplasmic Potential
Domain153 – 22876Cache
Domain303 – 35553HAMP
Domain374 – 610237Methyl-accepting transducer

Amino acid modifications

Modified residue3701Glutamate methyl ester (Glu) By similarity
Modified residue5941Glutamate methyl ester (Gln) By similarity
Modified residue6291Glutamate methyl ester (Glu) By similarity
Modified residue6361Glutamate methyl ester (Glu) By similarity

Sequences

Sequence LengthMass (Da)Tools
P39217 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 91215F86293D7425

FASTA66271,536
        10         20         30         40         50         60 
MGKFIQWIKQ PSISKPLIAA FLAVLILPVG VLAYFSYQSA WNALDRELIS SAKGNVEELN 

        70         80         90        100        110        120 
STLQNKLEDK VKAIDYYSET VDKDILLGKN KTLLKEKFKQ YTTLNDDVGA IYAASEDKKL 

       130        140        150        160        170        180 
YKYPDSGVPK GFDPTGRDWY KQAVAEKGQA VFSEPYTDEA TGDIVVTISK QLKDGSGVIA 

       190        200        210        220        230        240 
LDLNLDEVLT ASKRIKIGKE GFAFITTGNK KYIAHPTIKP GTTGSGDWTN QVYSKKEGSF 

       250        260        270        280        290        300 
EYTFEGKEKK MAFTTNKLTG WKIAGTYFVS ELQDASSPVL NTAVIILCVS IVIGGILILY 

       310        320        330        340        350        360 
IIRAITKPLR KLVSTSAKIS SGDLTEVIDI HSKNEFGQLG ESFNEMSASL RSVIGVIQTS 

       370        380        390        400        410        420 
VENVASSSEE LTASAAQTSK ATEHITLAIE QFSDGNEAQS EKLETSSNHL SQMNEGISKV 

       430        440        450        460        470        480 
AQASSTITKS SIQSSEAAGS GEKLVEHTVG QMKTIDQSVQ KAEAVVKGLE TKSQDITSIL 

       490        500        510        520        530        540 
NVINGIADQT NLLALNAAIE AARAGEYGRG FSVVAEEVRK LAVQSADSAK EIEGLIQEIV 

       550        560        570        580        590        600 
REISTSLSMF QSVNHEVKEG LQITDQTAES FKQIYEMTTQ ISGELQNLNA TVEQLSAGSQ 

       610        620        630        640        650        660 
EVSSAVEDIS AVAKESSAGI QDIAASAEEQ LASMEEISSS AETLANMAEE LQDITKKFKI 


ES

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis."
Hanlon D.W., Ordal G.W.
J. Biol. Chem. 269:14038-14046(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / OI1085.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L29189 Genomic DNA. Translation: AAA20557.1.
AL009126 Genomic DNA. Translation: CAB15101.1.
PIRD54078.
RefSeqNP_391001.1. NC_000964.3.

3D structure databases

ProteinModelPortalP39217.
SMRP39217. Positions 303-355, 380-661.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU31230.

Proteomic databases

PaxDbP39217.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15101; CAB15101; BSU31230.
GeneID937152.
KEGGbsu:BSU31230.
PATRIC18978204. VBIBacSub10457_3265.

Organism-specific databases

GenoListBSU31230. [Micado]

Phylogenomic databases

eggNOGCOG0840.
HOGENOMHOG000083278.
KOK03406.
OMAIDYYSET.
ProtClustDBCLSK2301479.

Enzyme and pathway databases

BioCycBSUB:BSU31230-MONOMER.

Family and domain databases

InterProIPR004010. Cache_domain.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR003660. HAMP_linker_domain.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
SMARTSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
PROSITEPS00538. CHEMOTAXIS_TRANSDUC_1. False negative.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTLPB_BACSU
AccessionPrimary (citable) accession number: P39217
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents