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P39214 (MCPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-accepting chemotaxis protein McpA
Alternative name(s):
H1
Gene names
Name:mcpA
Ordered Locus Names:BSU31240
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpA is required for taxis towards glucose and alpha-methylglucoside. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Deamidated by CheD on Gln-593 and Gln-594, producing glutamate residues. The glutamate residues are then methylated. Other additional sites are deamidated and methylated as well. Ref.1

Sequence similarities

Contains 1 cache domain.

Contains 1 HAMP domain.

Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionTransducer
   PTMMethylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processchemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661Methyl-accepting chemotaxis protein McpA
PRO_0000110556

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721Helical; Potential
Topological domain38 – 281244Extracellular Potential
Transmembrane282 – 30221Helical; Potential
Topological domain303 – 661359Cytoplasmic Potential
Domain152 – 22877Cache
Domain303 – 35553HAMP
Domain374 – 610237Methyl-accepting transducer

Amino acid modifications

Modified residue3701Glutamate methyl ester (Glu) Probable
Modified residue5931Deamidated glutamine Ref.4
Modified residue5931Glutamate methyl ester (Gln)
Modified residue5941Deamidated glutamine Ref.4
Modified residue5941Glutamate methyl ester (Gln) Ref.1
Modified residue6291Glutamate methyl ester (Glu) Probable
Modified residue6361Glutamate methyl ester (Glu) Probable

Experimental info

Mutagenesis5931Q → A: No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-594. Ref.4
Mutagenesis5941Q → A: No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-593. Ref.4
Sequence conflict951A → R in AAA20556. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39214 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: E46A09AB33604141

FASTA66172,314
        10         20         30         40         50         60 
MKKILQLIKQ RSITRKLLVS FLSILIIPVV ILAIFAYQSA SSSLDRQMMG SALENVQQLN 

        70         80         90        100        110        120 
EIINTSIGEK ENSADYFSEW LTKEKYNAKS NASIAEKFSQ YISINKDVES IYTSDTKGHF 

       130        140        150        160        170        180 
TRYPDLPMPS GYNPVERDWY KKAVANKGKV VITDPYKTAS TNTMVVTIAQ QTKDGSGVIA 

       190        200        210        220        230        240 
INMTIENLLK TTKKVNIGTQ GYAFIMTKDK KVVAHPNEQS GTELKGDWLD KMLSADKGDF 

       250        260        270        280        290        300 
QYTMDGDKKK MAFDTNKLTG WKIGGTMYLD EIHEAAQPVL HLALIVLAAA IIIGIIVMTL 

       310        320        330        340        350        360 
IIRSITTPLK QLVGSSKRIS EGDLTETIDI RSKDELGELG KSFNNMASSL RSLIHAIQDS 

       370        380        390        400        410        420 
VDNVAASSEE LTASAAQTSK ATEHITLAIE QFSNGNEKQN ENIETAAEHI YQMNDGLTNM 

       430        440        450        460        470        480 
AQASEVITDS SVQSTEIASE GGKLVHQTVG QMNVIDKSVK EAEQVVRGLE TKSKDITNIL 

       490        500        510        520        530        540 
RVINGIADQT NLLALNAAIE AARAGEYGRG FSVVAEEVRK LAVQSADSAK EIEGLIIEIV 

       550        560        570        580        590        600 
KEINTSLGMF QSVNQEVQTG LDITDKTEMS FKRISEMTNQ IAGELQNMSA TVQQLSASSE 

       610        620        630        640        650        660 
EVSGASEHIA SISKESSAHI QDIAASAEEQ LASMEEISSS AETLSSMAEE LRDMTKRFKI 


E 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis."
Hanlon D.W., Ordal G.W.
J. Biol. Chem. 269:14038-14046(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], METHYLATION AT GLU-370; GLN-594; GLU-629 AND GLU-636, FUNCTION.
Strain: 168 / OI1085.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 95.
[4]"Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis."
Kristich C.J., Ordal G.W.
J. Biol. Chem. 277:25356-25362(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 589-595, DEAMIDATION AT GLN-593 AND GLN-594 BY CHED, MUTAGENESIS OF GLN-593 AND GLN-594.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29189 Genomic DNA. Translation: AAA20556.1.
AL009126 Genomic DNA. Translation: CAB15102.2.
PIRB54078.
RefSeqNP_391002.2. NC_000964.3.

3D structure databases

ProteinModelPortalP39214.
SMRP39214. Positions 303-355, 364-659.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP39214. 23 interactions.
STRING224308.BSU31240.

Proteomic databases

PaxDbP39214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15102; CAB15102; BSU31240.
GeneID937154.
KEGGbsu:BSU31240.
PATRIC18978206. VBIBacSub10457_3266.

Organism-specific databases

GenoListBSU31240. [Micado]

Phylogenomic databases

eggNOGCOG0840.
HOGENOMHOG000083278.
KOK03406.
OrthoDBEOG67HJTT.
ProtClustDBCLSK2301479.

Enzyme and pathway databases

BioCycBSUB:BSU31240-MONOMER.

Family and domain databases

InterProIPR004010. Cache_domain.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR003660. HAMP_linker_domain.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
SMARTSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
PROSITEPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMCPA_BACSU
AccessionPrimary (citable) accession number: P39214
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 7, 2009
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList