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Protein

Methyl-accepting chemotaxis protein McpA

Gene

mcpA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpA is required for taxis towards glucose and alpha-methylglucoside.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

BioCyciBSUB:BSU31240-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-accepting chemotaxis protein McpA
Alternative name(s):
H1
Gene namesi
Name:mcpA
Ordered Locus Names:BSU31240
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 281ExtracellularSequence analysisAdd BLAST244
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Topological domaini303 – 661CytoplasmicSequence analysisAdd BLAST359

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi593Q → A: No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-594. 1 Publication1
Mutagenesisi594Q → A: No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-593. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001105561 – 661Methyl-accepting chemotaxis protein McpAAdd BLAST661

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei370Glutamate methyl ester (Glu)1 Publication1
Modified residuei593Deamidated glutamine1 Publication1
Modified residuei594Deamidated glutamine1 Publication1
Modified residuei594Glutamate methyl ester (Gln)2 Publications1
Modified residuei629Glutamate methyl ester (Glu)1 Publication1
Modified residuei636Glutamate methyl ester (Glu)1 Publication1

Post-translational modificationi

Deamidated by CheD on Gln-593 and Gln-594, producing glutamate residues. The glutamate residues are then methylated. Other additional sites are deamidated and methylated as well.2 Publications

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP39214.

Interactioni

Protein-protein interaction databases

IntActiP39214. 23 interactors.
STRINGi224308.Bsubs1_010100016981.

Structurei

3D structure databases

ProteinModelPortaliP39214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 228CacheAdd BLAST77
Domaini303 – 355HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini374 – 610Methyl-accepting transducerPROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 methyl-accepting transducer domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000083278.
InParanoidiP39214.
KOiK03406.
OMAiRIEDIQH.
PhylomeDBiP39214.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
InterProiIPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR033479. dCache_1.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
IPR029151. Sensor-like.
[Graphical view]
PfamiPF02743. dCache_1. 1 hit.
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
[Graphical view]
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
PROSITEiPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKILQLIKQ RSITRKLLVS FLSILIIPVV ILAIFAYQSA SSSLDRQMMG
60 70 80 90 100
SALENVQQLN EIINTSIGEK ENSADYFSEW LTKEKYNAKS NASIAEKFSQ
110 120 130 140 150
YISINKDVES IYTSDTKGHF TRYPDLPMPS GYNPVERDWY KKAVANKGKV
160 170 180 190 200
VITDPYKTAS TNTMVVTIAQ QTKDGSGVIA INMTIENLLK TTKKVNIGTQ
210 220 230 240 250
GYAFIMTKDK KVVAHPNEQS GTELKGDWLD KMLSADKGDF QYTMDGDKKK
260 270 280 290 300
MAFDTNKLTG WKIGGTMYLD EIHEAAQPVL HLALIVLAAA IIIGIIVMTL
310 320 330 340 350
IIRSITTPLK QLVGSSKRIS EGDLTETIDI RSKDELGELG KSFNNMASSL
360 370 380 390 400
RSLIHAIQDS VDNVAASSEE LTASAAQTSK ATEHITLAIE QFSNGNEKQN
410 420 430 440 450
ENIETAAEHI YQMNDGLTNM AQASEVITDS SVQSTEIASE GGKLVHQTVG
460 470 480 490 500
QMNVIDKSVK EAEQVVRGLE TKSKDITNIL RVINGIADQT NLLALNAAIE
510 520 530 540 550
AARAGEYGRG FSVVAEEVRK LAVQSADSAK EIEGLIIEIV KEINTSLGMF
560 570 580 590 600
QSVNQEVQTG LDITDKTEMS FKRISEMTNQ IAGELQNMSA TVQQLSASSE
610 620 630 640 650
EVSGASEHIA SISKESSAHI QDIAASAEEQ LASMEEISSS AETLSSMAEE
660
LRDMTKRFKI E
Length:661
Mass (Da):72,314
Last modified:July 7, 2009 - v2
Checksum:iE46A09AB33604141
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95A → R in AAA20556 (PubMed:8188684).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29189 Genomic DNA. Translation: AAA20556.1.
AL009126 Genomic DNA. Translation: CAB15102.2.
PIRiB54078.
RefSeqiNP_391002.2. NC_000964.3.
WP_003244077.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15102; CAB15102; BSU31240.
GeneIDi937154.
KEGGibsu:BSU31240.
PATRICi18978206. VBIBacSub10457_3266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29189 Genomic DNA. Translation: AAA20556.1.
AL009126 Genomic DNA. Translation: CAB15102.2.
PIRiB54078.
RefSeqiNP_391002.2. NC_000964.3.
WP_003244077.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliP39214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP39214. 23 interactors.
STRINGi224308.Bsubs1_010100016981.

Proteomic databases

PaxDbiP39214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15102; CAB15102; BSU31240.
GeneIDi937154.
KEGGibsu:BSU31240.
PATRICi18978206. VBIBacSub10457_3266.

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000083278.
InParanoidiP39214.
KOiK03406.
OMAiRIEDIQH.
PhylomeDBiP39214.

Enzyme and pathway databases

BioCyciBSUB:BSU31240-MONOMER.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
InterProiIPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR033479. dCache_1.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
IPR029151. Sensor-like.
[Graphical view]
PfamiPF02743. dCache_1. 1 hit.
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
[Graphical view]
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
PROSITEiPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCPA_BACSU
AccessioniPrimary (citable) accession number: P39214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 7, 2009
Last modified: October 5, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.