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Protein

50S ribosomal protein L3 glutamine methyltransferase

Gene

prmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the 50S ribosomal protein L3 on 'Gln-150'. Does not methylate the translation termination release factors RF1 and RF2.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + [ribosomal protein L3]-L-glutamine = S-adenosyl-L-homocysteine + [ribosomal protein L3]-N5-methyl-L-glutamine.1 Publication

GO - Molecular functioni

GO - Biological processi

  • peptidyl-glutamine methylation Source: EcoCyc
  • protein methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12449-MONOMER
MetaCyc:EG12449-MONOMER
BRENDAi2.1.1.298 2026

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L3 glutamine methyltransferase (EC:2.1.1.298)
Short name:
L3 MTase
Alternative name(s):
N5-glutamine methyltransferase PrmB
Gene namesi
Name:prmB
Synonyms:yfcB
Ordered Locus Names:b2330, JW5841
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12449 prmB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880031 – 31050S ribosomal protein L3 glutamine methyltransferaseAdd BLAST310

Proteomic databases

PaxDbiP39199
PRIDEiP39199

Interactioni

Protein-protein interaction databases

BioGridi4261060, 26 interactors
DIPiDIP-11976N
IntActiP39199, 8 interactors
STRINGi316385.ECDH10B_2492

Structurei

3D structure databases

ProteinModelPortaliP39199
SMRiP39199
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C76 Bacteria
COG2890 LUCA
HOGENOMiHOG000076275
InParanoidiP39199
KOiK07320
OMAiVLVCEVG
PhylomeDBiP39199

Family and domain databases

HAMAPiMF_02125 L3_methyltr_PrmB, 1 hit
InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR004556 Modification_methylase_HemK
IPR017127 Ribosome_L3_Gln-N5_MeTrfase
IPR029063 SAM-dependent_MTases
IPR007848 Small_mtfrase_dom
PfamiView protein in Pfam
PF05175 MTS, 1 hit
PIRSFiPIRSF037167 Mtase_YfcB_prd, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00536 hemK_fam, 1 hit
TIGR03533 L3_gln_methyl, 1 hit

Sequencei

Sequence statusi: Complete.

P39199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKIFVDEAV NELQTIQDML RWSVSRFSAA NIWYGHGTDN PWDEAVQLVL
60 70 80 90 100
PSLYLPLDIP EDMRTARLTS SEKHRIVERV IRRVNERIPV AYLTNKAWFC
110 120 130 140 150
GHEFYVDERV LVPRSPIGEL INNKFAGLIS KQPQHILDMC TGSGCIAIAC
160 170 180 190 200
AYAFPDAEVD AVDISPDALA VAEQNIEEHG LIHNVIPIRS DLFRDLPKVQ
210 220 230 240 250
YDLIVTNPPY VDAEDMSDLP NEYRHEPELG LASGTDGLKL TRRILGNAAD
260 270 280 290 300
YLADDGVLIC EVGNSMVHLM EQYPDVPFTW LEFDNGGDGV FMLTKEQLIA
310
AREHFAIYKD
Length:310
Mass (Da):35,002
Last modified:September 26, 2001 - v3
Checksum:i3EE8F2D45AFC3760
GO

Sequence cautioni

The sequence M33021 differs from that shown. Reason: Frameshift at positions 214 and 222.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75390.2
AP009048 Genomic DNA Translation: BAA16188.2
M27714 Genomic DNA No translation available.
Y00720 Genomic DNA No translation available.
M33021 Genomic DNA No translation available.
PIRiH65005
RefSeqiNP_416833.4, NC_000913.3
WP_001300582.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75390; AAC75390; b2330
BAA16188; BAA16188; BAA16188
GeneIDi946805
KEGGiecj:JW5841
eco:b2330
PATRICifig|511145.12.peg.2426

Similar proteinsi

Entry informationi

Entry nameiPRMB_ECOLI
AccessioniPrimary (citable) accession number: P39199
Secondary accession number(s): P76939, P78252
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 26, 2001
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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