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Protein

50S ribosomal protein L3 glutamine methyltransferase

Gene

prmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the 50S ribosomal protein L3 on 'Gln-150'. Does not methylate the translation termination release factors RF1 and RF2.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + [ribosomal protein L3]-L-glutamine = S-adenosyl-L-homocysteine + [ribosomal protein L3]-N(5)-methyl-L-glutamine.1 Publication

GO - Molecular functioni

GO - Biological processi

  • peptidyl-glutamine methylation Source: EcoCyc
  • protein methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG12449-MONOMER.
ECOL316407:JW5841-MONOMER.
MetaCyc:EG12449-MONOMER.
BRENDAi2.1.1.298. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L3 glutamine methyltransferase (EC:2.1.1.298)
Short name:
L3 MTase
Alternative name(s):
N5-glutamine methyltransferase PrmB
Gene namesi
Name:prmB
Synonyms:yfcB
Ordered Locus Names:b2330, JW5841
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12449. prmB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31031050S ribosomal protein L3 glutamine methyltransferasePRO_0000088003Add
BLAST

Proteomic databases

EPDiP39199.
PaxDbiP39199.
PRIDEiP39199.

Interactioni

Protein-protein interaction databases

BioGridi4261060. 5 interactions.
DIPiDIP-11976N.
IntActiP39199. 8 interactions.
MINTiMINT-1267343.
STRINGi511145.b2330.

Structurei

3D structure databases

ProteinModelPortaliP39199.
SMRiP39199. Positions 61-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C76. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076275.
InParanoidiP39199.
KOiK07320.
OMAiVLVCEVG.
OrthoDBiEOG68Q0SZ.
PhylomeDBiP39199.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02125. L3_methyltr_PrmB.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR017127. Ribosome_L3_Gln-N5_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
PIRSFiPIRSF037167. Mtase_YfcB_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03533. L3_gln_methyl. 1 hit.

Sequencei

Sequence statusi: Complete.

P39199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKIFVDEAV NELQTIQDML RWSVSRFSAA NIWYGHGTDN PWDEAVQLVL
60 70 80 90 100
PSLYLPLDIP EDMRTARLTS SEKHRIVERV IRRVNERIPV AYLTNKAWFC
110 120 130 140 150
GHEFYVDERV LVPRSPIGEL INNKFAGLIS KQPQHILDMC TGSGCIAIAC
160 170 180 190 200
AYAFPDAEVD AVDISPDALA VAEQNIEEHG LIHNVIPIRS DLFRDLPKVQ
210 220 230 240 250
YDLIVTNPPY VDAEDMSDLP NEYRHEPELG LASGTDGLKL TRRILGNAAD
260 270 280 290 300
YLADDGVLIC EVGNSMVHLM EQYPDVPFTW LEFDNGGDGV FMLTKEQLIA
310
AREHFAIYKD
Length:310
Mass (Da):35,002
Last modified:September 26, 2001 - v3
Checksum:i3EE8F2D45AFC3760
GO

Sequence cautioni

The sequence M33021 differs from that shown. Reason: Frameshift at positions 214 and 222. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75390.2.
AP009048 Genomic DNA. Translation: BAA16188.2.
M27714 Genomic DNA. No translation available.
Y00720 Genomic DNA. No translation available.
M33021 Genomic DNA. No translation available.
PIRiH65005.
RefSeqiNP_416833.4. NC_000913.3.
WP_001300582.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75390; AAC75390; b2330.
BAA16188; BAA16188; BAA16188.
GeneIDi946805.
KEGGiecj:JW5841.
eco:b2330.
PATRICi32120033. VBIEscCol129921_2426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75390.2.
AP009048 Genomic DNA. Translation: BAA16188.2.
M27714 Genomic DNA. No translation available.
Y00720 Genomic DNA. No translation available.
M33021 Genomic DNA. No translation available.
PIRiH65005.
RefSeqiNP_416833.4. NC_000913.3.
WP_001300582.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39199.
SMRiP39199. Positions 61-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261060. 5 interactions.
DIPiDIP-11976N.
IntActiP39199. 8 interactions.
MINTiMINT-1267343.
STRINGi511145.b2330.

Proteomic databases

EPDiP39199.
PaxDbiP39199.
PRIDEiP39199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75390; AAC75390; b2330.
BAA16188; BAA16188; BAA16188.
GeneIDi946805.
KEGGiecj:JW5841.
eco:b2330.
PATRICi32120033. VBIEscCol129921_2426.

Organism-specific databases

EchoBASEiEB2343.
EcoGeneiEG12449. prmB.

Phylogenomic databases

eggNOGiENOG4105C76. Bacteria.
COG2890. LUCA.
HOGENOMiHOG000076275.
InParanoidiP39199.
KOiK07320.
OMAiVLVCEVG.
OrthoDBiEOG68Q0SZ.
PhylomeDBiP39199.

Enzyme and pathway databases

BioCyciEcoCyc:EG12449-MONOMER.
ECOL316407:JW5841-MONOMER.
MetaCyc:EG12449-MONOMER.
BRENDAi2.1.1.298. 2026.

Miscellaneous databases

PROiP39199.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_02125. L3_methyltr_PrmB.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR017127. Ribosome_L3_Gln-N5_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
PIRSFiPIRSF037167. Mtase_YfcB_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03533. L3_gln_methyl. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Isolation, characterization and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli."
    Charles I.G., Lamb H.K., Pickard D., Dougan G., Hawkins A.R.
    J. Gen. Microbiol. 136:353-358(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 172-310.
  5. "The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa."
    White P.J., Millar G., Coggins J.R.
    Biochem. J. 251:313-322(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 159-310.
    Strain: K12.
  6. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors."
    Heurgue-Hamard V., Champ S., Engstroem A., Ehrenberg M., Buckingham R.H.
    EMBO J. 21:769-778(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME.
    Strain: K12.

Entry informationi

Entry nameiPRMB_ECOLI
AccessioniPrimary (citable) accession number: P39199
Secondary accession number(s): P76939, P78252
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 26, 2001
Last modified: March 16, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.