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Protein

Antigen 43

Gene

flu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls colony form variation and autoaggregation. May function as an adhesin.1 Publication

Enzyme and pathway databases

BioCyciEcoCyc:G7080-MONOMER.
ECOL316407:JW1982-MONOMER.

Protein family/group databases

TCDBi1.B.12.8.2. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen 43
Short name:
AG43
Alternative name(s):
Fluffing protein
Cleaved into the following 2 chains:
Gene namesi
Name:flu
Synonyms:yeeQ, yzzX
Ordered Locus Names:b2000, JW1982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12686. flu.

Subcellular locationi

Antigen 43 :
Chain Antigen 43 alpha chain :
  • Secreted
  • Cell surface

  • Note: The cell surface component is about 60 kDa and can be released by mild heat treatment.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 52521 PublicationAdd
BLAST
Chaini53 – 1039987Antigen 43PRO_0000387572Add
BLAST
Chaini53 – 551499Antigen 43 alpha chainPRO_0000002696Add
BLAST
Chaini552 – 1039488Antigen 43 beta chainPRO_0000002697Add
BLAST

Proteomic databases

PaxDbiP39180.
PRIDEiP39180.

Interactioni

Protein-protein interaction databases

DIPiDIP-2892N.
IntActiP39180. 4 interactions.
STRINGi511145.b2000.

Structurei

3D structure databases

ProteinModelPortaliP39180.
SMRiP39180. Positions 53-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini737 – 1039303AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage. Finally, the mature protein remains tightly associated with the bacterium (Probable).Curated

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105F4K. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000009569.
KOiK12687.
OMAiIWQHLSL.
PhylomeDBiP39180.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF16168. AIDA. 3 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
[Graphical view]
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR04415. O_hepto_targRPT. 5 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRHLNTCYR LVWNHMTGAF VVASELARAR GKRGGVAVAL SLAAVTSLPV
60 70 80 90 100
LAADIVVHPG ETVNGGTLAN HDNQIVFGTT NGMTISTGLE YGPDNEANTG
110 120 130 140 150
GQWVQDGGTA NKTTVTSGGL QRVNPGGSVS DTVISAGGGQ SLQGRAVNTT
160 170 180 190 200
LNGGEQWMHE GAIATGTVIN DKGWQVVKPG TVATDTVVNT GAEGGPDAEN
210 220 230 240 250
GDTGQFVRGD AVRTTINKNG RQIVRAEGTA NTTVVYAGGD QTVHGHALDT
260 270 280 290 300
TLNGGYQYVH NGGTASDTVV NSDGWQIVKN GGVAGNTTVN QKGRLQVDAG
310 320 330 340 350
GTATNVTLKQ GGALVTSTAA TVTGINRLGA FSVVEGKADN VVLENGGRLD
360 370 380 390 400
VLTGHTATNT RVDDGGTLDV RNGGTATTVS MGNGGVLLAD SGAAVSGTRS
410 420 430 440 450
DGKAFSIGGG QADALMLEKG SSFTLNAGDT ATDTTVNGGL FTARGGTLAG
460 470 480 490 500
TTTLNNGAIL TLSGKTVNND TLTIREGDAL LQGGSLTGNG SVEKSGSGTL
510 520 530 540 550
TVSNTTLTQK AVNLNEGTLT LNDSTVTTDV IAQRGTALKL TGSTVLNGAI
560 570 580 590 600
DPTNVTLASG ATWNIPDNAT VQSVVDDLSH AGQIHFTSTR TGKFVPATLK
610 620 630 640 650
VKNLNGQNGT ISLRVRPDMA QNNADRLVID GGRATGKTIL NLVNAGNSAS
660 670 680 690 700
GLATSGKGIQ VVEAINGATT EEGAFVQGNR LQAGAFNYSL NRDSDESWYL
710 720 730 740 750
RSENAYRAEV PLYASMLTQA MDYDRIVAGS RSHQTGVNGE NNSVRLSIQG
760 770 780 790 800
GHLGHDNNGG IARGATPESS GSYGFVRLEG DLMRTEVAGM SVTAGVYGAA
810 820 830 840 850
GHSSVDVKDD DGSRAGTVRD DAGSLGGYLN LVHTSSGLWA DIVAQGTRHS
860 870 880 890 900
MKASSDNNDF RARGWGWLGS LETGLPFSIT DNLMLEPQLQ YTWQGLSLDD
910 920 930 940 950
GKDNAGYVKF GHGSAQHVRA GFRLGSHNDM TFGEGTSSRA PLRDSAKHSV
960 970 980 990 1000
SELPVNWWVQ PSVIRTFSSR GDMRVGTSTA GSGMTFSPSQ NGTSLDLQAG
1010 1020 1030
LEARVRENIT LGVQAGYAHS VSGSSAEGYN GQATLNVTF
Length:1,039
Mass (Da):106,825
Last modified:October 11, 2004 - v3
Checksum:iABC16C46C264EAED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 633ETV → TTT AA sequence (PubMed:9298646).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21K → N in strain: ML 308-225.
Natural varianti41 – 422SL → FF in strain: ML 308-225.
Natural varianti46 – 461T → K in strain: ML 308-225.
Natural varianti157 – 1571W → L in strain: ML 308-225.
Natural varianti188 – 1881V → F in strain: ML 308-225.
Natural varianti303 – 3053ATN → STI in strain: ML 308-225.
Natural varianti320 – 3201A → T in strain: ML 308-225.
Natural varianti372 – 3721N → Q in strain: ML 308-225.
Natural varianti493 – 4931E → V in strain: ML 308-225.
Natural varianti497 – 4971S → N in strain: ML 308-225.
Natural varianti585 – 5851H → Y in strain: ML 308-225.
Natural varianti709 – 7091E → K in strain: ML 308-225.
Natural varianti721 – 7211M → T in strain: ML 308-225.
Natural varianti751 – 7533GHL → SHF in strain: ML 308-225.
Natural varianti803 – 8031S → P in strain: ML 308-225.
Natural varianti815 – 8151A → V in strain: ML 308-225.
Natural varianti829 – 8357LNLVHTS → MNLIYNA in strain: ML 308-225.
Natural varianti845 – 8473QGT → LGA in strain: ML 308-225.
Natural varianti855 – 8551S → T in strain: ML 308-225.
Natural varianti888 – 8881Q → L in strain: ML 308-225.
Natural varianti1025 – 10251S → I in strain: ML 308-225.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24429 Genomic DNA. Translation: AAB47869.1.
U00096 Genomic DNA. Translation: AAT48141.1.
AP009048 Genomic DNA. Translation: BAA15825.2.
RefSeqiWP_000820410.1. NZ_LN832404.1.
YP_026164.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48141; AAT48141; b2000.
BAA15825; BAA15825; BAA15825.
GeneIDi946540.
KEGGiecj:JW1982.
eco:b2000.
PATRICi32119333. VBIEscCol129921_2076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24429 Genomic DNA. Translation: AAB47869.1.
U00096 Genomic DNA. Translation: AAT48141.1.
AP009048 Genomic DNA. Translation: BAA15825.2.
RefSeqiWP_000820410.1. NZ_LN832404.1.
YP_026164.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP39180.
SMRiP39180. Positions 53-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-2892N.
IntActiP39180. 4 interactions.
STRINGi511145.b2000.

Protein family/group databases

TCDBi1.B.12.8.2. the autotransporter-1 (at-1) family.

Proteomic databases

PaxDbiP39180.
PRIDEiP39180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48141; AAT48141; b2000.
BAA15825; BAA15825; BAA15825.
GeneIDi946540.
KEGGiecj:JW1982.
eco:b2000.
PATRICi32119333. VBIEscCol129921_2076.

Organism-specific databases

EchoBASEiEB2550.
EcoGeneiEG12686. flu.

Phylogenomic databases

eggNOGiENOG4105F4K. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000009569.
KOiK12687.
OMAiIWQHLSL.
PhylomeDBiP39180.

Enzyme and pathway databases

BioCyciEcoCyc:G7080-MONOMER.
ECOL316407:JW1982-MONOMER.

Miscellaneous databases

PROiP39180.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF16168. AIDA. 3 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
[Graphical view]
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR04415. O_hepto_targRPT. 5 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAG43_ECOLI
AccessioniPrimary (citable) accession number: P39180
Secondary accession number(s): P75614
, P76360, P97241, Q46771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.