Probable L,D-transpeptidase ErfK/SrfK precursor - Escherichia coli (strain K12)

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P39176 (ERFK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable L,D-transpeptidase ErfK/SrfK
EC=2.-.-.-

Gene names

Name:	erfK
Synonyms:	yeeG, yzzT
Ordered Locus Names:	b1990, JW1968

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp. Ref.6
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit structure	Interacts with DsbG. Ref.7
Subcellular location	Periplasm Potential.
Disruption phenotype	Similtaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan. Complementation with erfK restores some of this anchoring. Ref.6
Sequence similarities	Belongs to the YkuD family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosyltransferase Hydrolase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Gram-negative-bacterium-type cell wall biogenesis Inferred from mutant phenotype Ref.6. Source: EcoCyc cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan-protein cross-linking Inferred from mutant phenotype Ref.6. Source: EcoCyc regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type carboxypeptidase activity Inferred from mutant phenotype Ref.6. Source: EcoCyc peptidoglycan L,D-transpeptidase activity Inferred from mutant phenotype. Source: EcoCyc transferase activity, transferring amino-acyl groups Inferred from mutant phenotype Ref.6. Source: EcoCyc transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Ref.5

Chain

22 – 310

289

Probable L,D-transpeptidase ErfK/SrfK

PRO_0000021194

Sites

Active site

207

Potential

Experimental info

Sequence conflict

28 – 29

PE → TD AA sequence Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P39176 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: ABF26AC3E6F550AB
Show »

FASTA

310

34,411

        10         20         30         40         50         60 
MRRVNILCSF ALLFASHTSL AVTYPLPPEG SRLVGQSFTV TVPDHNTQPL ETFAAQYGQG 

        70         80         90        100        110        120 
LSNMLEANPG ADVFLPKSGS QLTIPQQLIL PDTVRKGIVV NVAEMRLYYY PPDSNTVEVF 

       130        140        150        160        170        180 
PIGIGQAGRE TPRNWVTTVE RKQEAPTWTP TPNTRREYAK RGESLPAFVP AGPDNPMGLY 

       190        200        210        220        230        240 
AIYIGRLYAI HGTNANFGIG LRVSQGCIRL RNDDIKYLFD NVPVGTRVQI IDQPVKYTTE 

       250        260        270        280        290        300 
PDGSNWLEVH EPLSRNRAEY ESDRKVPLPV TPSLRAFING QEVDVNRANA ALQRRSGMPV 

       310 
QISSGSRQMF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." Lawrence J.G., Roth J.R. J. Bacteriol. 177:6371-6380(1995) [PubMed: 7592411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-33. Strain: K12 / EMG2.
[6]	"Identification of the L,D-transpeptidases for peptidoglycan cross-linking in Escherichia coli." Magnet S., Dubost L., Marie A., Arthur M., Gutmann L. J. Bacteriol. 190:4782-4785(2008) [PubMed: 18456808] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: K12 / BW25113.
[7]	"A periplasmic reducing system protects single cysteine residues from oxidation." Depuydt M., Leonard S.E., Vertommen D., Denoncin K., Morsomme P., Wahni K., Messens J., Carroll K.S., Collet J.F. Science 326:1109-1111(2009) [PubMed: 19965429] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH DSBG. Strain: K12 / MC1000 / ATCC 39531.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33333 Genomic DNA. Translation: AAA78909.1. U00096 Genomic DNA. Translation: AAC75051.1. AP009048 Genomic DNA. Translation: BAA15807.1.
PIR	E64963.
RefSeq	NP_416494.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P39176.
SMR	P39176. Positions 35-87, 96-230.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9522N.
IntAct	P39176. 2 interactions.
MINT	MINT-1229758.
Protein family/group databases
MEROPS	C82.A02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001745; EBESCP00000001745; EBESCG00000001438. EBESCT00000014631; EBESCP00000013922; EBESCG00000013692.
GeneID	945273.
GenomeReviews	Gene locus JW1968 in contig AP009048_GR. Gene locus b1990 in contig U00096_GR.
KEGG	ecj:JW1968. eco:b1990.
PATRIC	32119311. VBIEscCol129921_2065.
Organism-specific databases
EchoBASE	EB2546.
EcoGene	EG12682. erfK.
Phylogenomic databases
eggNOG	COG1376.
GeneTree	EBGT00050000008974.
HOGENOM	HBG295778.
OMA	SVEVLPI.
PhylomeDB	P39176.
ProtClustDB	PRK10190.
Enzyme and pathway databases
BioCyc	EcoCyc:G7073-MONOMER.
Gene expression databases
Genevestigator	P39176.
Family and domain databases
InterPro	IPR005490. LD_TPept_cat_dom. [Graphical view]
Pfam	PF03734. YkuD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERFK_ECOLI

Accession

Primary (citable) accession number: P39176

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents