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Protein

Probable L,D-transpeptidase ErfK/SrfK

Gene

erfK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei207Sequence analysis1

GO - Molecular functioni

  • cysteine-type carboxypeptidase activity Source: EcoCyc
  • peptidoglycan L,D-transpeptidase activity Source: EcoCyc
  • transferase activity, transferring amino-acyl groups Source: EcoCyc
  • transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • Gram-negative-bacterium-type cell wall biogenesis Source: EcoCyc
  • peptidoglycan-protein cross-linking Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Hydrolase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7073-MONOMER
MetaCyc:G7073-MONOMER
UniPathwayiUPA00219

Protein family/group databases

MEROPSiC82.A02

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L,D-transpeptidase ErfK/SrfK (EC:2.-.-.-)
Gene namesi
Name:erfK
Synonyms:yeeG, yzzT
Ordered Locus Names:b1990, JW1968
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12682 erfK

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan. Complementation with erfK restores some of this anchoring.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000002119422 – 310Probable L,D-transpeptidase ErfK/SrfKAdd BLAST289

Proteomic databases

PaxDbiP39176
PRIDEiP39176

Interactioni

Subunit structurei

Interacts with DsbG.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pheSP083124EBI-555707,EBI-555676

Protein-protein interaction databases

BioGridi4259382, 19 interactors
DIPiDIP-9522N
IntActiP39176, 2 interactors
STRINGi316385.ECDH10B_2134

Structurei

3D structure databases

ProteinModelPortaliP39176
SMRiP39176
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the YkuD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108PXI Bacteria
COG1376 LUCA
HOGENOMiHOG000274378
InParanoidiP39176
KOiK16291
OMAiKQEGPTW
PhylomeDBiP39176

Family and domain databases

Gene3Di2.40.440.10, 1 hit
InterProiView protein in InterPro
IPR005490 LD_TPept_cat_dom
IPR038063 Transpep_catalytic_dom
PfamiView protein in Pfam
PF03734 YkuD, 1 hit
SUPFAMiSSF141523 SSF141523, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRVNILCSF ALLFASHTSL AVTYPLPPEG SRLVGQSFTV TVPDHNTQPL
60 70 80 90 100
ETFAAQYGQG LSNMLEANPG ADVFLPKSGS QLTIPQQLIL PDTVRKGIVV
110 120 130 140 150
NVAEMRLYYY PPDSNTVEVF PIGIGQAGRE TPRNWVTTVE RKQEAPTWTP
160 170 180 190 200
TPNTRREYAK RGESLPAFVP AGPDNPMGLY AIYIGRLYAI HGTNANFGIG
210 220 230 240 250
LRVSQGCIRL RNDDIKYLFD NVPVGTRVQI IDQPVKYTTE PDGSNWLEVH
260 270 280 290 300
EPLSRNRAEY ESDRKVPLPV TPSLRAFING QEVDVNRANA ALQRRSGMPV
310
QISSGSRQMF
Length:310
Mass (Da):34,411
Last modified:November 1, 1995 - v2
Checksum:iABF26AC3E6F550AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28 – 29PE → TD AA sequence (PubMed:9298646).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA Translation: AAA78909.1
U00096 Genomic DNA Translation: AAC75051.1
AP009048 Genomic DNA Translation: BAA15807.1
PIRiE64963
RefSeqiNP_416494.1, NC_000913.3
WP_001350523.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75051; AAC75051; b1990
BAA15807; BAA15807; BAA15807
GeneIDi945273
KEGGiecj:JW1968
eco:b1990
PATRICifig|511145.12.peg.2065

Similar proteinsi

Entry informationi

Entry nameiERFK_ECOLI
AccessioniPrimary (citable) accession number: P39176
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health