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Protein

Probable L,D-transpeptidase ErfK/SrfK

Gene

erfK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei207 – 2071Sequence analysis

GO - Molecular functioni

  • cysteine-type carboxypeptidase activity Source: EcoCyc
  • peptidoglycan L,D-transpeptidase activity Source: EcoCyc
  • transferase activity, transferring amino-acyl groups Source: EcoCyc
  • transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • Gram-negative-bacterium-type cell wall biogenesis Source: EcoCyc
  • peptidoglycan-protein cross-linking Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7073-MONOMER.
ECOL316407:JW1968-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

MEROPSiC82.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L,D-transpeptidase ErfK/SrfK (EC:2.-.-.-)
Gene namesi
Name:erfK
Synonyms:yeeG, yzzT
Ordered Locus Names:b1990, JW1968
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12682. erfK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan. Complementation with erfK restores some of this anchoring.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 310289Probable L,D-transpeptidase ErfK/SrfKPRO_0000021194Add
BLAST

Proteomic databases

EPDiP39176.
PaxDbiP39176.
PRIDEiP39176.

Interactioni

Subunit structurei

Interacts with DsbG.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pheSP083123EBI-555707,EBI-555676

Protein-protein interaction databases

BioGridi4259382. 6 interactions.
DIPiDIP-9522N.
IntActiP39176. 2 interactions.
MINTiMINT-1229758.
STRINGi511145.b1990.

Structurei

3D structure databases

ProteinModelPortaliP39176.
SMRiP39176. Positions 21-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the YkuD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108PXI. Bacteria.
COG1376. LUCA.
HOGENOMiHOG000274378.
InParanoidiP39176.
KOiK16291.
OMAiQWITSIK.
OrthoDBiEOG6CVVCJ.
PhylomeDBiP39176.

Family and domain databases

InterProiIPR005490. LD_TPept_cat_dom.
[Graphical view]
PfamiPF03734. YkuD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRVNILCSF ALLFASHTSL AVTYPLPPEG SRLVGQSFTV TVPDHNTQPL
60 70 80 90 100
ETFAAQYGQG LSNMLEANPG ADVFLPKSGS QLTIPQQLIL PDTVRKGIVV
110 120 130 140 150
NVAEMRLYYY PPDSNTVEVF PIGIGQAGRE TPRNWVTTVE RKQEAPTWTP
160 170 180 190 200
TPNTRREYAK RGESLPAFVP AGPDNPMGLY AIYIGRLYAI HGTNANFGIG
210 220 230 240 250
LRVSQGCIRL RNDDIKYLFD NVPVGTRVQI IDQPVKYTTE PDGSNWLEVH
260 270 280 290 300
EPLSRNRAEY ESDRKVPLPV TPSLRAFING QEVDVNRANA ALQRRSGMPV
310
QISSGSRQMF
Length:310
Mass (Da):34,411
Last modified:November 1, 1995 - v2
Checksum:iABF26AC3E6F550AB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292PE → TD AA sequence (PubMed:9298646).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78909.1.
U00096 Genomic DNA. Translation: AAC75051.1.
AP009048 Genomic DNA. Translation: BAA15807.1.
PIRiE64963.
RefSeqiNP_416494.1. NC_000913.3.
WP_001350523.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75051; AAC75051; b1990.
BAA15807; BAA15807; BAA15807.
GeneIDi945273.
KEGGiecj:JW1968.
eco:b1990.
PATRICi32119311. VBIEscCol129921_2065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33333 Genomic DNA. Translation: AAA78909.1.
U00096 Genomic DNA. Translation: AAC75051.1.
AP009048 Genomic DNA. Translation: BAA15807.1.
PIRiE64963.
RefSeqiNP_416494.1. NC_000913.3.
WP_001350523.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39176.
SMRiP39176. Positions 21-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259382. 6 interactions.
DIPiDIP-9522N.
IntActiP39176. 2 interactions.
MINTiMINT-1229758.
STRINGi511145.b1990.

Protein family/group databases

MEROPSiC82.A02.

Proteomic databases

EPDiP39176.
PaxDbiP39176.
PRIDEiP39176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75051; AAC75051; b1990.
BAA15807; BAA15807; BAA15807.
GeneIDi945273.
KEGGiecj:JW1968.
eco:b1990.
PATRICi32119311. VBIEscCol129921_2065.

Organism-specific databases

EchoBASEiEB2546.
EcoGeneiEG12682. erfK.

Phylogenomic databases

eggNOGiENOG4108PXI. Bacteria.
COG1376. LUCA.
HOGENOMiHOG000274378.
InParanoidiP39176.
KOiK16291.
OMAiQWITSIK.
OrthoDBiEOG6CVVCJ.
PhylomeDBiP39176.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:G7073-MONOMER.
ECOL316407:JW1968-MONOMER.

Miscellaneous databases

PROiP39176.

Family and domain databases

InterProiIPR005490. LD_TPept_cat_dom.
[Graphical view]
PfamiPF03734. YkuD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli."
    Lawrence J.G., Roth J.R.
    J. Bacteriol. 177:6371-6380(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-33.
    Strain: K12 / EMG2.
  6. "Identification of the L,D-transpeptidases for peptidoglycan cross-linking in Escherichia coli."
    Magnet S., Dubost L., Marie A., Arthur M., Gutmann L.
    J. Bacteriol. 190:4782-4785(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.
  7. "A periplasmic reducing system protects single cysteine residues from oxidation."
    Depuydt M., Leonard S.E., Vertommen D., Denoncin K., Morsomme P., Wahni K., Messens J., Carroll K.S., Collet J.F.
    Science 326:1109-1111(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH DSBG.
    Strain: K12 / MC1000 / ATCC 39531.

Entry informationi

Entry nameiERFK_ECOLI
AccessioniPrimary (citable) accession number: P39176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.