Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

High-affinity zinc uptake system protein ZnuA

Gene

znuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the high-affinity zinc uptake transport system.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601ZincBy similarity
Metal bindingi143 – 1431ZincBy similarity
Metal bindingi207 – 2071ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport, Zinc transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ZNUA-MONOMER.
ECOL316407:JW5831-MONOMER.
MetaCyc:ZNUA-MONOMER.

Protein family/group databases

TCDBi3.A.1.15.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity zinc uptake system protein ZnuA
Gene namesi
Name:znuA
Synonyms:yebL, yzzP
Ordered Locus Names:b1857, JW5831
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12678. znuA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26263 PublicationsAdd
BLAST
Chaini27 – 310284High-affinity zinc uptake system protein ZnuAPRO_0000031875Add
BLAST

Proteomic databases

EPDiP39172.
PaxDbiP39172.
PRIDEiP39172.

2D gel databases

SWISS-2DPAGEP39172.

Expressioni

Inductioni

Maximally induced 30 minutes under zinc-limiting conditions, still induced in stationary phase.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260353. 6 interactions.
DIPiDIP-12949N.
STRINGi511145.b1857.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 325Combined sources
Helixi33 – 4311Combined sources
Turni44 – 463Combined sources
Beta strandi49 – 535Combined sources
Turni59 – 613Combined sources
Helixi68 – 747Combined sources
Beta strandi76 – 805Combined sources
Turni83 – 853Combined sources
Helixi87 – 893Combined sources
Helixi90 – 945Combined sources
Helixi98 – 1003Combined sources
Beta strandi101 – 1033Combined sources
Helixi104 – 1063Combined sources
Turni108 – 1103Combined sources
Helixi111 – 1133Combined sources
Helixi144 – 1463Combined sources
Helixi148 – 16518Combined sources
Helixi167 – 1693Combined sources
Helixi170 – 19526Combined sources
Helixi196 – 1983Combined sources
Beta strandi203 – 2086Combined sources
Helixi211 – 2177Combined sources
Beta strandi223 – 2286Combined sources
Beta strandi229 – 2313Combined sources
Helixi235 – 24713Combined sources
Beta strandi252 – 2554Combined sources
Helixi262 – 2676Combined sources
Turni268 – 2703Combined sources
Beta strandi274 – 2774Combined sources
Helixi291 – 30717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OGWX-ray1.83A/B1-310[»]
2OSVX-ray1.75A/B27-310[»]
2PRSX-ray1.70A/B27-310[»]
2PS0X-ray2.00A/B27-310[»]
2PS3X-ray2.47A/B27-310[»]
2PS9X-ray2.15A/B27-310[»]
ProteinModelPortaliP39172.
SMRiP39172. Positions 27-309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39172.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 13719His-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105NXV. Bacteria.
COG4531. LUCA.
HOGENOMiHOG000180311.
InParanoidiP39172.
KOiK09815.
OMAiLPGMHLR.
OrthoDBiEOG6PGK2R.
PhylomeDBiP39172.

Family and domain databases

InterProiIPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00690. ADHESNFAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHKKTLLFA ALSAALWGGA TQAADAAVVA SLKPVGFIAS AIADGVTETE
60 70 80 90 100
VLLPDGASEH DYSLRPSDVK RLQNADLVVW VGPEMEAFMQ KPVSKLPGAK
110 120 130 140 150
QVTIAQLEDV KPLLMKSIHG DDDDHDHAEK SDEDHHHGDF NMHLWLSPEI
160 170 180 190 200
ARATAVAIHG KLVELMPQSR AKLDANLKDF EAQLASTETQ VGNELAPLKG
210 220 230 240 250
KGYFVFHDAY GYFEKQFGLT PLGHFTVNPE IQPGAQRLHE IRTQLVEQKA
260 270 280 290 300
TCVFAEPQFR PAVVESVARG TSVRMGTLDP LGTNIKLGKT SYSEFLSQLA
310
NQYASCLKGD
Length:310
Mass (Da):33,777
Last modified:June 1, 2001 - v4
Checksum:i99500F04C6C3DBF0
GO

Sequence cautioni

The sequence AAA81030.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951L → V in AAA81030 (Ref. 1) Curated
Sequence conflicti262 – 2621A → P in AAA81030 (Ref. 1) Curated
Sequence conflicti289 – 2891K → E in AAA81030 (Ref. 1) Curated
Sequence conflicti306 – 3061C → S in AAA81030 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38702 Genomic DNA. Translation: AAA81030.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74927.2.
AP009048 Genomic DNA. Translation: BAA15665.2.
PIRiA64948.
RefSeqiNP_416371.4. NC_000913.3.
WP_001300644.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74927; AAC74927; b1857.
BAA15665; BAA15665; BAA15665.
GeneIDi946375.
KEGGiecj:JW5831.
eco:b1857.
PATRICi32119037. VBIEscCol129921_1936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38702 Genomic DNA. Translation: AAA81030.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74927.2.
AP009048 Genomic DNA. Translation: BAA15665.2.
PIRiA64948.
RefSeqiNP_416371.4. NC_000913.3.
WP_001300644.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OGWX-ray1.83A/B1-310[»]
2OSVX-ray1.75A/B27-310[»]
2PRSX-ray1.70A/B27-310[»]
2PS0X-ray2.00A/B27-310[»]
2PS3X-ray2.47A/B27-310[»]
2PS9X-ray2.15A/B27-310[»]
ProteinModelPortaliP39172.
SMRiP39172. Positions 27-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260353. 6 interactions.
DIPiDIP-12949N.
STRINGi511145.b1857.

Protein family/group databases

TCDBi3.A.1.15.5. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP39172.

Proteomic databases

EPDiP39172.
PaxDbiP39172.
PRIDEiP39172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74927; AAC74927; b1857.
BAA15665; BAA15665; BAA15665.
GeneIDi946375.
KEGGiecj:JW5831.
eco:b1857.
PATRICi32119037. VBIEscCol129921_1936.

Organism-specific databases

EchoBASEiEB2543.
EcoGeneiEG12678. znuA.

Phylogenomic databases

eggNOGiENOG4105NXV. Bacteria.
COG4531. LUCA.
HOGENOMiHOG000180311.
InParanoidiP39172.
KOiK09815.
OMAiLPGMHLR.
OrthoDBiEOG6PGK2R.
PhylomeDBiP39172.

Enzyme and pathway databases

BioCyciEcoCyc:ZNUA-MONOMER.
ECOL316407:JW5831-MONOMER.
MetaCyc:ZNUA-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP39172.
PROiP39172.

Family and domain databases

InterProiIPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00690. ADHESNFAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Robison K., O'Keeffe T., Church G.M.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / EMG2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-38.
    Strain: K12 / EMG2.
  6. Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.
    Submitted (FEB-1996) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 27-36.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. Cited for: PROTEIN SEQUENCE OF 27-30.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. "The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli."
    Patzer S.I., Hantke K.
    Mol. Microbiol. 28:1199-1210(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. Cited for: INDUCTION.
    Strain: BW21135.

Entry informationi

Entry nameiZNUA_ECOLI
AccessioniPrimary (citable) accession number: P39172
Secondary accession number(s): P76284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.