P39172 (ZNUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High-affinity zinc uptake system protein ZnuA

Gene names

Name:	znuA
Synonyms:	yebL, yzzP
Ordered Locus Names:	b1857, JW5831

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the high-affinity zinc uptake transport system.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 9 family.
Sequence caution	The sequence AAA81030.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro zinc ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Ref.5 Ref.6 Ref.7

Chain

27 – 310

284

High-affinity zinc uptake system protein ZnuA

PRO_0000031875

Regions

Compositional bias

119 – 137

19

His-rich

Sites

Metal binding

60

1

Zinc By similarity

Metal binding

143

1

Zinc By similarity

Metal binding

207

1

Zinc By similarity

Experimental info

Sequence conflict

195

1

L → V in AAA81030. Ref.1

Sequence conflict

262

1

A → P in AAA81030. Ref.1

Sequence conflict

289

1

K → E in AAA81030. Ref.1

Sequence conflict

306

1

C → S in AAA81030. Ref.1

Secondary structure

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310

Helix Strand Turn

Details...

Beta strand

28 – 32

5

Helix

33 – 43

11

Turn

44 – 46

3

Beta strand

49 – 53

5

Turn

59 – 61

3

Helix

68 – 74

7

Beta strand

76 – 80

5

Turn

83 – 85

3

Helix

87 – 89

3

Helix

90 – 94

5

Helix

98 – 100

3

Beta strand

101 – 103

3

Helix

104 – 106

3

Turn

108 – 110

3

Helix

111 – 113

3

Helix

144 – 146

3

Helix

148 – 165

18

Helix

167 – 169

3

Helix

170 – 195

26

Helix

196 – 198

3

Beta strand

203 – 208

6

Helix

211 – 217

7

Beta strand

223 – 228

6

Beta strand

229 – 231

3

Helix

235 – 247

13

Beta strand

252 – 255

4

Helix

262 – 267

6

Turn

268 – 270

3

Beta strand

274 – 277

4

Helix

291 – 307

17

Sequences

	Sequence		Length	Mass (Da)
	P39172 [UniParc]. Last modified June 1, 2001. Version 4. Checksum: 99500F04C6C3DBF0	FASTA	310	33,777
	10 20 30 40 50 60 MLHKKTLLFA ALSAALWGGA TQAADAAVVA SLKPVGFIAS AIADGVTETE VLLPDGASEH 70 80 90 100 110 120 DYSLRPSDVK RLQNADLVVW VGPEMEAFMQ KPVSKLPGAK QVTIAQLEDV KPLLMKSIHG 130 140 150 160 170 180 DDDDHDHAEK SDEDHHHGDF NMHLWLSPEI ARATAVAIHG KLVELMPQSR AKLDANLKDF 190 200 210 220 230 240 EAQLASTETQ VGNELAPLKG KGYFVFHDAY GYFEKQFGLT PLGHFTVNPE IQPGAQRLHE 250 260 270 280 290 300 IRTQLVEQKA TCVFAEPQFR PAVVESVARG TSVRMGTLDP LGTNIKLGKT SYSEFLSQLA 310 NQYASCLKGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Robison K., O'Keeffe T., Church G.M. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / EMG2.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-38. Strain: K12 / EMG2.
[6]	Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 27-36. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Protein identification with N and C-terminal sequence tags in proteome projects." Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C., Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L., Hochstrasser D.F. J. Mol. Biol. 278:599-608(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-30. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli." Patzer S.I., Hantke K. Mol. Microbiol. 28:1199-1210(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U38702 Genomic DNA. Translation: AAA81030.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74927.2.
AP009048 Genomic DNA. Translation: BAA15665.2.

PIR

A64948.

RefSeq

NP_416371.4. NC_000913.2.
YP_490119.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OGW	X-ray	1.83	A/B	1-310	[»]
2OSV	X-ray	1.75	A/B	27-310	[»]
2PRS	X-ray	1.70	A/B	27-310	[»]
2PS0	X-ray	2.00	A/B	27-310	[»]
2PS3	X-ray	2.47	A/B	27-310	[»]
2PS9	X-ray	2.15	A/B	27-310	[»]

ProteinModelPortal

P39172.

SMR

P39172. Positions 27-309.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12949N.

STRING

511145.b1857.

Protein family/group databases

TCDB

3.A.1.15.5. ATP-binding cassette (ABC) superfamily.

2D gel databases

SWISS-2DPAGE

P39172.

Proteomic databases

PaxDb

P39172.

PRIDE

P39172.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74927; AAC74927; b1857.
BAA15665; BAA15665; BAA15665.

GeneID

12931359.
946375.

KEGG

ecj:Y75_p1833.
eco:b1857.

PATRIC

32119037. VBIEscCol129921_1936.

Organism-specific databases

EchoBASE

EB2543.

EcoGene

EG12678. znuA.

Phylogenomic databases

eggNOG

COG4531.

HOGENOM

HOG000180311.

KO

K09815.

OMA

MPQNKDK.

ProtClustDB

PRK09545.

Enzyme and pathway databases

BioCyc

EcoCyc:ZNUA-MONOMER.
ECOL316407:JW5831-MONOMER.

Gene expression databases

Genevestigator

P39172.

Family and domain databases

InterPro

IPR006128. Lipoprotein_4.
IPR006127. Perip_solute-bd_prot_fam.
[Graphical view]

Pfam

PF01297. SBP_bac_9. 1 hit.
[Graphical view]

PRINTS

PR00690. ADHESNFAMILY.

ProtoNet

Search...

Other

EvolutionaryTrace

P39172.

Entry information

Entry name

ZNUA_ECOLI

Accession

Primary (citable) accession number: P39172
Secondary accession number(s): P76284

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 107 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program