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Protein

Glutathione-specific gamma-glutamylcyclotransferase

Gene

chaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.By similarity

Catalytic activityi

Glutathione = 5-oxoproline + cysteinylglycine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG12403-MONOMER.
ECOL316407:JW1209-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferaseBy similarity (EC:2.3.2.-By similarity)
Short name:
Gamma-GCGBy similarity
Alternative name(s):
Cation transport regulatory protein ChaC1 Publication
Gene namesi
Name:chaCImported
Ordered Locus Names:b1218, JW1209
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12403. chaC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Glutathione-specific gamma-glutamylcyclotransferasePRO_0000089636Add
BLAST

Proteomic databases

PaxDbiP39163.
PRIDEiP39163.

Interactioni

Protein-protein interaction databases

BioGridi4261606. 11 interactions.
IntActiP39163. 2 interactions.
STRINGi511145.b1218.

Structurei

3D structure databases

ProteinModelPortaliP39163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 546Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108NH8. Bacteria.
COG3703. LUCA.
HOGENOMiHOG000264265.
InParanoidiP39163.
KOiK07232.
OMAiWNPALEY.
PhylomeDBiP39163.

Family and domain databases

Gene3Di3.10.490.10. 1 hit.
InterProiIPR013024. Butirosin_synth_BtrG-like.
IPR006840. ChaC.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.

Sequencei

Sequence statusi: Complete.

P39163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITRDFLMNA DCKTAFGAIE ESLLWSAEQR AASLAATLAC RPDEGPVWIF
60 70 80 90 100
GYGSLMWNPA LEFTESCTGT LVGWHRAFCL RLTAGRGTAH QPGRMLALKE
110 120 130 140 150
GGRTTGVAYR LPEETLEQEL TLLWKREMIT GCYLPTWCQL DLDDGRTVNA
160 170 180 190 200
IVFIMDPRHP EYESDTRAQV IAPLIAAASG PLGTNAQYLF SLEQELIKLG
210 220 230
MQDDGLNDLL VSVKKLLAEN FPDGVLRPGF A
Length:231
Mass (Da):25,512
Last modified:December 1, 2000 - v3
Checksum:i9D7088566D553A7C
GO

Sequence cautioni

The sequence AAA20198 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA36076 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151A → V in AAA20198 (Ref. 1) Curated
Sequence conflicti79 – 791C → F in AAA20198 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28709 Genomic DNA. Translation: AAA20198.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74302.2.
AP009048 Genomic DNA. Translation: BAA36076.1. Different initiation.
PIRiG64868.
RefSeqiNP_415736.2. NC_000913.3.
WP_001336325.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74302; AAC74302; b1218.
BAA36076; BAA36076; BAA36076.
GeneIDi945793.
KEGGiecj:JW1209.
eco:b1218.
PATRICi32117694. VBIEscCol129921_1269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28709 Genomic DNA. Translation: AAA20198.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74302.2.
AP009048 Genomic DNA. Translation: BAA36076.1. Different initiation.
PIRiG64868.
RefSeqiNP_415736.2. NC_000913.3.
WP_001336325.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP39163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261606. 11 interactions.
IntActiP39163. 2 interactions.
STRINGi511145.b1218.

Proteomic databases

PaxDbiP39163.
PRIDEiP39163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74302; AAC74302; b1218.
BAA36076; BAA36076; BAA36076.
GeneIDi945793.
KEGGiecj:JW1209.
eco:b1218.
PATRICi32117694. VBIEscCol129921_1269.

Organism-specific databases

EchoBASEiEB2303.
EcoGeneiEG12403. chaC.

Phylogenomic databases

eggNOGiENOG4108NH8. Bacteria.
COG3703. LUCA.
HOGENOMiHOG000264265.
InParanoidiP39163.
KOiK07232.
OMAiWNPALEY.
PhylomeDBiP39163.

Enzyme and pathway databases

BioCyciEcoCyc:EG12403-MONOMER.
ECOL316407:JW1209-MONOMER.

Miscellaneous databases

PROiP39163.

Family and domain databases

Gene3Di3.10.490.10. 1 hit.
InterProiIPR013024. Butirosin_synth_BtrG-like.
IPR006840. ChaC.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHAC_ECOLI
AccessioniPrimary (citable) accession number: P39163
Secondary accession number(s): P77176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.