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Protein

Glutathione-specific gamma-glutamylcyclotransferase

Gene

chaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.1 Publication

Catalytic activityi

Glutathione = L-cysteinylglycine + 5-oxo-L-proline.1 Publication

Kineticsi

kcat is 13 min(-1) for glutathione.1 Publication
  1. KM=3.1 mM for glutathione1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei127Proton acceptorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • bacterial-type flagellum-dependent cell motility Source: EcoCyc
    • glutathione catabolic process Source: GO_Central

    Keywordsi

    Molecular functionLyase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12403-MONOMER
    MetaCyc:EG12403-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-specific gamma-glutamylcyclotransferase1 Publication (EC:4.3.2.71 Publication)
    Short name:
    Gamma-GCG1 Publication
    Alternative name(s):
    Cation transport regulatory protein ChaC1 Publication
    Gene namesi
    Name:chaCImported
    Ordered Locus Names:b1218, JW1209
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12403 chaC

    Subcellular locationi

    GO - Cellular componenti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000896361 – 231Glutathione-specific gamma-glutamylcyclotransferaseAdd BLAST231

    Proteomic databases

    PaxDbiP39163
    PRIDEiP39163

    Interactioni

    Protein-protein interaction databases

    BioGridi4261606, 11 interactors
    IntActiP39163, 2 interactors
    STRINGi316385.ECDH10B_1277

    Structurei

    3D structure databases

    ProteinModelPortaliP39163
    SMRiP39163
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni49 – 54Substrate bindingBy similarity6

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4108NH8 Bacteria
    COG3703 LUCA
    HOGENOMiHOG000264265
    InParanoidiP39163
    KOiK07232
    OMAiDFPYEEK
    PhylomeDBiP39163

    Family and domain databases

    CDDicd06661 GGCT_like, 1 hit
    InterProiView protein in InterPro
    IPR006840 ChaC
    IPR013024 GGCT-like
    IPR036568 GGCT-like_sf
    PANTHERiPTHR12192 PTHR12192, 1 hit
    PfamiView protein in Pfam
    PF04752 ChaC, 1 hit
    SUPFAMiSSF110857 SSF110857, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P39163-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MITRDFLMNA DCKTAFGAIE ESLLWSAEQR AASLAATLAC RPDEGPVWIF
    60 70 80 90 100
    GYGSLMWNPA LEFTESCTGT LVGWHRAFCL RLTAGRGTAH QPGRMLALKE
    110 120 130 140 150
    GGRTTGVAYR LPEETLEQEL TLLWKREMIT GCYLPTWCQL DLDDGRTVNA
    160 170 180 190 200
    IVFIMDPRHP EYESDTRAQV IAPLIAAASG PLGTNAQYLF SLEQELIKLG
    210 220 230
    MQDDGLNDLL VSVKKLLAEN FPDGVLRPGF A
    Length:231
    Mass (Da):25,512
    Last modified:December 1, 2000 - v3
    Checksum:i9D7088566D553A7C
    GO

    Sequence cautioni

    The sequence AAA20198 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA36076 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti15A → V in AAA20198 (Ref. 1) Curated1
    Sequence conflicti79C → F in AAA20198 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L28709 Genomic DNA Translation: AAA20198.1 Different initiation.
    U00096 Genomic DNA Translation: AAC74302.2
    AP009048 Genomic DNA Translation: BAA36076.1 Different initiation.
    PIRiG64868
    RefSeqiNP_415736.2, NC_000913.3
    WP_001336325.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74302; AAC74302; b1218
    BAA36076; BAA36076; BAA36076
    GeneIDi945793
    KEGGiecj:JW1209
    eco:b1218
    PATRICifig|511145.12.peg.1269

    Similar proteinsi

    Entry informationi

    Entry nameiCHAC_ECOLI
    AccessioniPrimary (citable) accession number: P39163
    Secondary accession number(s): P77176
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 1, 2000
    Last modified: May 23, 2018
    This is version 121 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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