Reviewed, UniProtKB/Swiss-Prot P39160 (UXUB_ECOLI)

Names and origin

Protein names

Recommended name:
    D-mannonate oxidoreductase
    EC=1.1.1.57
Alternative name(s):
    Fructuronate reductase

Gene names

Name:	uxuB
Ordered Locus Names:	b4323, JW4286

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-mannonate + NAD+ = D-fructuronate + NADH.
Pathway	Carbohydrate metabolism; pentose and glucuronate interconversion.
Sequence similarities	Belongs to the mannitol dehydrogenase family. UxuB subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-glucuronate catabolic process Inferred from mutant phenotype. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro fructuronate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	D-mannonate oxidoreductase		PRO_0000170747
Regions
	Nucleotide binding	25 – 36	12	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39160-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7835638682AF7B87	FASTA	486	53,580
	10 20 30 40 50 60 MTTIVDSNLP VARPSWDHSR LESRIVHLGC GAFHRAHQAL YTHHLLESTD SDWGICEVNL 70 80 90 100 110 120 MPGNDRVLIE NLKKQQLLYT VAEKGAESTE LKIIGSMKEA LHPEIDGCEG ILNAMARPQT 130 140 150 160 170 180 AIVSLTVTEK GYCADAASGQ LDLNNPLIKH DLENPTAPKS AIGYIVEALR LRREKGLKAF 190 200 210 220 230 240 TVMSCDNVRE NGHVAKVAVL GLAQARDPQL AAWIEENVTF PCTMVDRIVP AATPETLQEI 250 260 270 280 290 300 ADQLGVYDPC AIACEPFRQW VIEDNFVNGR PDWDKVGAQF VADVVPFEMM KLRMLNGSHS 310 320 330 340 350 360 FLAYLGYLGG YETIADTVTN PAYRKAAFAL MMQEQAPTLS MPEGTDLNAY ATLLIERFSN 370 380 390 400 410 420 PSLRHRTWQI AMDGSQKLPQ RLLDPVRLHL QNGGSWRHLA LGVAGWMRYT QGVDEQGNAI 430 440 450 460 470 480 DVVDPMLAEF QKINAQYQGA DRVKALLGLS GIFADDLPQN ADFVGAVTAA YQQLCERGAR ECVAAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mizobuchi K. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13329 Genomic DNA. Translation: BAA02591.1. U14003 Genomic DNA. Translation: AAA97219.1. U00096 Genomic DNA. Translation: AAC77279.1. AP009048 Genomic DNA. Translation: BAE78316.1.
PIR	S56548.
RefSeq	AP_004815.1. NP_418743.1.
3D structure databases
SMR	P39160. Positions 10-486.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11109N.
STRING	P39160.
Genome annotation databases
GeneID	946795.
GenomeReviews	Gene locus JW4286 in contig AP009048_GR. Gene locus b4323 in contig U00096_GR.
KEGG	ecj:JW4286. eco:b4323.
Organism-specific databases
EchoBASE	EB4150.
EcoGene	EG20248. uxuB.
CMR	Search...
Phylogenomic databases
eggNOG	COG0246.
HOGENOM	HBG510279.
OMA	GSDWGIC.
Enzyme and pathway databases
BioCyc	EcoCyc:MANNONOXIDOREDUCT-MONOMER. ECOL168927:B4323-MONOMER. MetaCyc:MANNONOXIDOREDUCT-MONOMER.
Gene expression databases
Genevestigator	P39160.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR013118. Mannitol_DH_C. IPR000669. Mannitol_DH_core. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UXUB_ECOLI

Accession

Primary (citable) accession number: P39160
Secondary accession number(s): Q2M5Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)