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Protein

4'-phosphopantetheinyl transferase Sfp

Gene

sfp

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide antibiotics surfactin and plipastatin B1.1 Publication

Caution

Strain 168 and its derivatives encode a truncated, inactive version of this protein. The sequence shown here corresponds to that of strain ATCC 21332 which is active.Curated

Catalytic activityi

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].1 Publication

Cofactori

Kineticsi

kcat is 7.2 min(-1) with S.mobaraensis apo-PCP BlmI as substrate and 0.89 min(-1) with S.glaucescens apo-ACP TcmM as substrate.1 Publication
  1. KM=3.7 µM for Streptomyces mobaraensis apo-PCP BlmI1 Publication
  2. KM=11 µM for Streptomyces glaucescens apo-ACP TcmM1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi107Magnesium1
    Metal bindingi109Magnesium1
    Metal bindingi151Magnesium1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processAntibiotic biosynthesis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13919

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4'-phosphopantetheinyl transferase Sfp (EC:2.7.8.71 Publication)
    Alternative name(s):
    Surfactin synthase-activating enzyme
    Gene namesi
    Name:sfp
    Synonyms:lpa-8
    Ordered Locus Names:BSU03570
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi105G → A: Almost no activity. 1 Publication1
    Mutagenesisi105G → D: Loss of activity. 1 Publication1
    Mutagenesisi107D → A: Loss of activity. 1 Publication1
    Mutagenesisi107D → E: 3000-fold reduction in activity, but no change in substrate affinity. 1 Publication1
    Mutagenesisi147W → A: 24-fold reduction in activity, but no change in substrate affinity. 1 Publication1
    Mutagenesisi147W → F: 5-fold reduction in activity, but no change in substrate affinity. 1 Publication1
    Mutagenesisi151E → A: Loss of activity. 1 Publication1
    Mutagenesisi155K → A: Loss of activity. 1 Publication1

    Chemistry databases

    DrugBankiDB01992 Coenzyme A

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002060771 – 2244'-phosphopantetheinyl transferase SfpAdd BLAST224

    Proteomic databases

    PaxDbiP39135
    PRIDEiP39135

    Interactioni

    Subunit structurei

    Monomer in solution.

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100002028

    Structurei

    Secondary structure

    1224
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 7Combined sources6
    Helixi14 – 21Combined sources8
    Helixi26 – 34Combined sources9
    Helixi38 – 58Combined sources21
    Helixi63 – 65Combined sources3
    Beta strandi85 – 91Combined sources7
    Beta strandi94 – 102Combined sources9
    Beta strandi105 – 110Combined sources6
    Helixi116 – 119Combined sources4
    Beta strandi120 – 123Combined sources4
    Helixi125 – 133Combined sources9
    Helixi136 – 157Combined sources22
    Helixi160 – 162Combined sources3
    Helixi165 – 167Combined sources3
    Beta strandi169 – 172Combined sources4
    Helixi174 – 176Combined sources3
    Beta strandi178 – 181Combined sources4
    Beta strandi190 – 194Combined sources5
    Beta strandi200 – 209Combined sources10
    Helixi220 – 224Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1QR0X-ray1.90A1-224[»]
    2GE0model-A1-224[»]
    2GE1model-A1-224[»]
    4MRTX-ray2.00A1-224[»]
    ProteinModelPortaliP39135
    SMRiP39135
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP39135

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni158 – 189Peptidyl carrier protein bindingSequence analysisAdd BLAST32

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105W8Z Bacteria
    COG2091 LUCA
    InParanoidiP39135

    Family and domain databases

    Gene3Di3.90.470.20, 3 hits
    InterProiView protein in InterPro
    IPR008278 4-PPantetheinyl_Trfase_dom
    IPR037143 4-PPantetheinyl_Trfase_dom_sf
    IPR004568 Ppantetheine-prot_Trfase_dom
    PfamiView protein in Pfam
    PF01648 ACPS, 1 hit
    SUPFAMiSSF56214 SSF56214, 2 hits
    TIGRFAMsiTIGR00556 pantethn_trn, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P39135-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIYGIYMDR PLSQEENERF MSFISPEKRE KCRRFYHKED AHRTLLGDVL
    60 70 80 90 100
    VRSVISRQYQ LDKSDIRFST QEYGKPCIPD LPDAHFNISH SGRWVICAFD
    110 120 130 140 150
    SQPIGIDIEK TKPISLEIAK RFFSKTEYSD LLAKDKDEQT DYFYHLWSMK
    160 170 180 190 200
    ESFIKQEGKG LSLPLDSFSV RLHQDGQVSI ELPDSHSPCY IKTYEVDPGY
    210 220
    KMAVCAAHPD FPEDITMVSY EELL
    Length:224
    Mass (Da):26,168
    Last modified:June 16, 2009 - v2
    Checksum:i0FEFB5D9D3534C68
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti118 – 119IA → MP in CAA46561 (PubMed:8355609).Curated2

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti22S → T in strain: oKB105. 1
    Natural varianti97C → G in strain: oKB105. 1
    Natural varianti157 – 224EGKGL…YEELL → GRQRLIASA in strain 168 and its derivatives, non surfactin-producing strains. Add BLAST68

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X63158 Genomic DNA Translation: CAA44858.1
    X65610 Genomic DNA Translation: CAA46561.1
    L17438 Unassigned DNA Translation: AAC36829.1
    X70356 Genomic DNA No translation available.
    D50562 Genomic DNA Translation: BAA09125.1
    AL009126 Genomic DNA Translation: CAB12151.2
    PIRiS20463

    Entry informationi

    Entry nameiSFP_BACSU
    AccessioniPrimary (citable) accession number: P39135
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: June 16, 2009
    Last modified: May 23, 2018
    This is version 120 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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