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Protein

UDP-N-acetylglucosamine 2-epimerase

Gene

mnaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of UDP-N-acetylglucosamine into UDP-N-acetylmannosamine, a precursor of the teichoic acid linkage unit.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine.

Pathwayi: poly(glycerol phosphate) teichoic acid biosynthesis

This protein is involved in the pathway poly(glycerol phosphate) teichoic acid biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway poly(glycerol phosphate) teichoic acid biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell wall biogenesis/degradation, Teichoic acid biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU35660-MONOMER.
MetaCyc:MONOMER-8808.
UniPathwayiUPA00827.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine 2-epimerase (EC:5.1.3.14)
Alternative name(s):
UDP-GlcNAc-2-epimerase
Gene namesi
Name:mnaA
Ordered Locus Names:BSU35660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69T → I in TS-21; temperature-sensitive, develops, at the nonpermissive temperature (47 degrees Celsius), a coccoid-like morphology, i.e. a phenotype associated with a deficient synthesis of poly(glycerol phosphate) teichoic acid. 1 Publication1
Mutagenesisi374P → L in TS-21; temperature-sensitive, develops, at the nonpermissive temperature (47 degrees Celsius), a coccoid-like morphology, i.e. a phenotype associated with a deficient synthesis of poly(glycerol phosphate) teichoic acid. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085321 – 380UDP-N-acetylglucosamine 2-epimeraseAdd BLAST380

Proteomic databases

PaxDbiP39131.
PRIDEiP39131.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019286.

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi13 – 26Combined sources14
Beta strandi32 – 38Combined sources7
Beta strandi41 – 43Combined sources3
Helixi44 – 53Combined sources10
Beta strandi58 – 60Combined sources3
Helixi70 – 87Combined sources18
Beta strandi91 – 96Combined sources6
Helixi100 – 111Combined sources12
Beta strandi115 – 119Combined sources5
Turni128 – 133Combined sources6
Helixi134 – 144Combined sources11
Beta strandi146 – 152Combined sources7
Helixi153 – 161Combined sources9
Helixi166 – 168Combined sources3
Beta strandi169 – 171Combined sources3
Helixi175 – 183Combined sources9
Helixi191 – 196Combined sources6
Beta strandi199 – 205Combined sources7
Helixi209 – 211Combined sources3
Helixi214 – 229Combined sources16
Beta strandi233 – 238Combined sources6
Helixi243 – 253Combined sources11
Beta strandi259 – 262Combined sources4
Helixi267 – 276Combined sources10
Beta strandi278 – 282Combined sources5
Helixi285 – 294Combined sources10
Beta strandi298 – 300Combined sources3
Helixi308 – 312Combined sources5
Beta strandi315 – 318Combined sources4
Helixi323 – 335Combined sources13
Helixi337 – 343Combined sources7
Helixi355 – 366Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O6CX-ray2.90A/B2-380[»]
4FKZX-ray1.69A/B1-380[»]
ProteinModelPortaliP39131.
SMRiP39131.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39131.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EAG. Bacteria.
COG0381. LUCA.
HOGENOMiHOG000076048.
InParanoidiP39131.
KOiK01791.
OMAiIMKERQT.
PhylomeDBiP39131.

Family and domain databases

CDDicd03786. GT1_UDP-GlcNAc_2-Epimerase. 1 hit.
InterProiIPR003331. UDP_GlcNAc_Epimerase_2_dom.
IPR029767. WecB-like.
[Graphical view]
PfamiPF02350. Epimerase_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00236. wecB. 1 hit.

Sequencei

Sequence statusi: Complete.

P39131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLKVMTVF GTRPEAIKMA PLVLELKKYP EIDSYVTVTA QHRQMLDQVL
60 70 80 90 100
DAFHIKPDFD LNIMKERQTL AEITSNALVR LDELFKDIKP DIVLVHGDTT
110 120 130 140 150
TTFAGSLAAF YHQIAVGHVE AGLRTGNKYS PFPEELNRQM TGAIADLHFA
160 170 180 190 200
PTGQAKDNLL KENKKADSIF VTGNTAIDAL NTTVRDGYSH PVLDQVGEDK
210 220 230 240 250
MILLTAHRRE NLGEPMENMF KAIRRIVGEF EDVQVVYPVH LNPVVREAAH
260 270 280 290 300
KHFGDSDRVH LIEPLEVIDF HNFAAKSHFI LTDSGGVQEE APSLGKPVLV
310 320 330 340 350
LRDTTERPEG VEAGTLKLAG TDEENIYQLA KQLLTDPDEY KKMSQASNPY
360 370 380
GDGEASRRIV EELLFHYGYR KEQPDSFTGK
Length:380
Mass (Da):42,634
Last modified:February 1, 1995 - v1
Checksum:i63A73C8D1E7D3B5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22516 Genomic DNA. Translation: CAA80240.1.
AL009126 Genomic DNA. Translation: CAB15583.1.
PIRiD70049.
RefSeqiNP_391446.1. NC_000964.3.
WP_003243178.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15583; CAB15583; BSU35660.
GeneIDi936209.
KEGGibsu:BSU35660.
PATRICi18979142. VBIBacSub10457_3733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22516 Genomic DNA. Translation: CAA80240.1.
AL009126 Genomic DNA. Translation: CAB15583.1.
PIRiD70049.
RefSeqiNP_391446.1. NC_000964.3.
WP_003243178.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O6CX-ray2.90A/B2-380[»]
4FKZX-ray1.69A/B1-380[»]
ProteinModelPortaliP39131.
SMRiP39131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019286.

Proteomic databases

PaxDbiP39131.
PRIDEiP39131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15583; CAB15583; BSU35660.
GeneIDi936209.
KEGGibsu:BSU35660.
PATRICi18979142. VBIBacSub10457_3733.

Phylogenomic databases

eggNOGiENOG4105EAG. Bacteria.
COG0381. LUCA.
HOGENOMiHOG000076048.
InParanoidiP39131.
KOiK01791.
OMAiIMKERQT.
PhylomeDBiP39131.

Enzyme and pathway databases

UniPathwayiUPA00827.
BioCyciBSUB:BSU35660-MONOMER.
MetaCyc:MONOMER-8808.

Miscellaneous databases

EvolutionaryTraceiP39131.

Family and domain databases

CDDicd03786. GT1_UDP-GlcNAc_2-Epimerase. 1 hit.
InterProiIPR003331. UDP_GlcNAc_Epimerase_2_dom.
IPR029767. WecB-like.
[Graphical view]
PfamiPF02350. Epimerase_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00236. wecB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNAA_BACSU
AccessioniPrimary (citable) accession number: P39131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.