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P39120 (CISY2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase 2

EC=2.3.3.16
Alternative name(s):
Citrate synthase II
Gene names
Name:citZ
Synonyms:citA2
Ordered Locus Names:BSU29140
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect.

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer Probable.

Induction

By decoyinine and nutrient depletion.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Citrate synthase 2
PRO_0000169929

Sites

Active site2571 By similarity
Active site3081 By similarity

Amino acid modifications

Modified residue2841Phosphoserine Ref.4

Experimental info

Sequence conflict391A → R in AAA96341. Ref.1
Sequence conflict268 – 2692KH → ND in AAA96341. Ref.1
Sequence conflict3321S → R in AAA96341. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39120 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: F19D7D4561EA55A7

FASTA37241,729
        10         20         30         40         50         60 
MTATRGLEGV VATTSSVSSI IDDTLTYVGY DIDDLTENAS FEEIIYLLWH LRLPNKKELE 

        70         80         90        100        110        120 
ELKQQLAKEA AVPQEIIEHF KSYSLENVHP MAALRTAISL LGLLDSEADT MNPEANYRKA 

       130        140        150        160        170        180 
IRLQAKVPGL VAAFSRIRKG LEPVEPREDY GIAENFLYTL NGEEPSPIEV EAFNKALILH 

       190        200        210        220        230        240 
ADHELNASTF TARVCVATLS DIYSGITAAI GALKGPLHGG ANEGVMKMLT EIGEVENAEP 

       250        260        270        280        290        300 
YIRAKLEKKE KIMGFGHRVY KHGDPRAKHL KEMSKRLTNL TGESKWYEMS IRIEDIVTSE 

       310        320        330        340        350        360 
KKLPPNVDFY SASVYHSLGI DHDLFTPIFA VSRMSGWLAH ILEQYDNNRL IRPRADYTGP 

       370 
DKQKFVPIEE RA 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two distinct Bacillus subtilis citrate synthase genes."
Jin S., Sonenshein A.L.
J. Bacteriol. 176:4669-4679(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21 AND 228-237.
Strain: 168 / SMY.
[2]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
Mol. Cell. Proteomics 6:697-707(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U05257 Genomic DNA. Translation: AAA96341.1.
AF008220 Genomic DNA. Translation: AAC00345.1.
AL009126 Genomic DNA. Translation: CAB14874.1.
PIRG69600.
RefSeqNP_390792.1. NC_000964.3.

3D structure databases

ProteinModelPortalP39120.
SMRP39120. Positions 3-371.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP39120. 1 interaction.
MINTMINT-8365474.
STRING224308.BSU29140.

PTM databases

PhosSiteP0802203.

Proteomic databases

PaxDbP39120.
PRIDEP39120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14874; CAB14874; BSU29140.
GeneID937381.
KEGGbsu:BSU29140.
PATRIC18977722. VBIBacSub10457_3049.

Organism-specific databases

GenoListBSU29140. [Micado]

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021225.
KOK01647.
OMAKFPTIVA.
OrthoDBEOG6P8TP4.
PhylomeDBP39120.

Enzyme and pathway databases

BioCycBSUB:BSU29140-MONOMER.
UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR011278. 2-MeCitrate/Citrate_synth_I.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01800. cit_synth_II. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY2_BACSU
AccessionPrimary (citable) accession number: P39120
Secondary accession number(s): O34435
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList