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P39118

- GLGB_BACSU

UniProt

P39118 - GLGB_BACSU

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei309 – 3091NucleophileBy similarity
    Active sitei352 – 3521Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciBSUB:BSU30980-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Alpha-(1->4)-glucan branching enzyme
    Glycogen branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    Ordered Locus Names:BSU30980
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU30980. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6276271,4-alpha-glucan branching enzyme GlgBPRO_0000188683Add
    BLAST

    Proteomic databases

    PaxDbiP39118.

    Expressioni

    Inductioni

    Expressed exclusively on media containing carbon sources that allow efficient sporulation.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi224308.BSU30980.

    Structurei

    3D structure databases

    ProteinModelPortaliP39118.
    SMRiP39118. Positions 18-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiGELSHAW.
    OrthoDBiEOG6JX7GT.
    PhylomeDBiP39118.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 2 hits.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39118-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAASPTAHD VYLFHEGSLF KSYQLFGSHY RELNGKSGYE FCVWAPHASE    50
    VRVAGDFNSW SGEEHVMHRV NDNGIWTLFI PGIGEKERYK YEIVTNNGEI 100
    RLKADPYAIY SEVRPNTASL TYDLEGYSWQ DQKWQKKQKA KTLYEKPVFI 150
    YELHLGSWKK HSDGRHYSYK ELSQTLIPYI KKHGFTHIEL LPVYEHPYDR 200
    SWGYQGTGYY SPTSRFGPPH DLMKFVDECH QQNIGVILDW VPGHFCKDAH 250
    GLYMFDGEPL YEYKEERDRE NWLWGTANFD LGKPEVHSFL ISNALYWAEF 300
    YHIDGFRVDA VANILYWPNQ DERHTNPYAV DFLKKLNQTM REAYPHVMMI 350
    AEDSTEWPQV TGAVEEGGLG FHYKWNMGWM NDVLKYMETP PEERRHCHQL 400
    ISFSLLYAFS EHFVLPFSHD EVVYGKKSLL NKMPGDYWQK FAQYRLLLGY 450
    MTVHPGKKLI FMGSEFAQFD EWKDTEQLDW FLDSFPMHQK ASVFTQDLLR 500
    FYQKSKILYE HDHRAQSFEW IDVHNDEQSI FSFIRYGQKH GEALVIICNF 550
    TPVVYHQYDV GVPFFTQYIE VLNSDSETYG GSGQINKKPL SAKKGALHHK 600
    PCYITMTIPP YGISILRAVK KRGEIKR 627
    Length:627
    Mass (Da):73,665
    Last modified:February 1, 1995 - v1
    Checksum:i64B0A5553B6767BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z25795 Genomic DNA. Translation: CAA81040.1.
    AF008220 Genomic DNA. Translation: AAC00214.1.
    AL009126 Genomic DNA. Translation: CAB15076.1.
    PIRiS40048.
    RefSeqiNP_390976.1. NC_000964.3.
    WP_004398803.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15076; CAB15076; BSU30980.
    GeneIDi937993.
    KEGGibsu:BSU30980.
    PATRICi18978102. VBIBacSub10457_3239.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z25795 Genomic DNA. Translation: CAA81040.1 .
    AF008220 Genomic DNA. Translation: AAC00214.1 .
    AL009126 Genomic DNA. Translation: CAB15076.1 .
    PIRi S40048.
    RefSeqi NP_390976.1. NC_000964.3.
    WP_004398803.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P39118.
    SMRi P39118. Positions 18-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU30980.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi P39118.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15076 ; CAB15076 ; BSU30980 .
    GeneIDi 937993.
    KEGGi bsu:BSU30980.
    PATRICi 18978102. VBIBacSub10457_3239.

    Organism-specific databases

    GenoListi BSU30980. [Micado ]

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi GELSHAW.
    OrthoDBi EOG6JX7GT.
    PhylomeDBi P39118.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci BSUB:BSU30980-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 2 hits.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation."
      Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
      Mol. Microbiol. 11:203-218(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
      Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
      Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiGLGB_BACSU
    AccessioniPrimary (citable) accession number: P39118
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3