ID   CAT8_YEAST              Reviewed;        1433 AA.
AC   P39113; D6W0A7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   27-MAR-2024, entry version 176.
DE   RecName: Full=Regulatory protein CAT8;
GN   Name=CAT8; Synonyms=MSP8; OrderedLocusNames=YMR280C;
GN   ORFNames=YM8021.06C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7891685; DOI=10.1128/mcb.15.4.1915;
RA   Hedges D., Proft M., Entian K.-D.;
RT   "CAT8, a new zinc cluster-encoding gene necessary for derepression of
RT   gluconeogenic enzymes in the yeast Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 15:1915-1922(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ENY.WA-1A;
RA   Boles E., Hettmann C., Zimmermann F.K.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- PTM: Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.
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DR   EMBL; X78344; CAA55139.1; -; Genomic_DNA.
DR   EMBL; Z49704; CAA89778.1; -; Genomic_DNA.
DR   EMBL; X94215; CAA63906.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10181.1; -; Genomic_DNA.
DR   PIR; S54587; S54587.
DR   RefSeq; NP_014007.1; NM_001182787.1.
DR   AlphaFoldDB; P39113; -.
DR   SMR; P39113; -.
DR   BioGRID; 35459; 111.
DR   DIP; DIP-4397N; -.
DR   MINT; P39113; -.
DR   STRING; 4932.YMR280C; -.
DR   GlyGen; P39113; 20 sites, 1 O-linked glycan (20 sites).
DR   iPTMnet; P39113; -.
DR   MaxQB; P39113; -.
DR   PaxDb; 4932-YMR280C; -.
DR   PeptideAtlas; P39113; -.
DR   EnsemblFungi; YMR280C_mRNA; YMR280C; YMR280C.
DR   GeneID; 855323; -.
DR   KEGG; sce:YMR280C; -.
DR   AGR; SGD:S000004893; -.
DR   SGD; S000004893; CAT8.
DR   VEuPathDB; FungiDB:YMR280C; -.
DR   eggNOG; ENOG502QTKQ; Eukaryota.
DR   HOGENOM; CLU_004300_1_0_1; -.
DR   InParanoid; P39113; -.
DR   OMA; FLAKCMI; -.
DR   OrthoDB; 1701898at2759; -.
DR   BioCyc; YEAST:G3O-32951-MONOMER; -.
DR   BioGRID-ORCS; 855323; 3 hits in 13 CRISPR screens.
DR   PRO; PR:P39113; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; P39113; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:SGD.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR   GO; GO:0061414; P:positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source; IMP:SGD.
DR   CDD; cd12148; fungal_TF_MHR; 1.
DR   CDD; cd00067; GAL4; 1.
DR   CDD; cd15485; ZIP_Cat8; 1.
DR   Gene3D; 4.10.240.10; Zn(2)-C6 fungal-type DNA-binding domain; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   InterPro; IPR001138; Zn2Cys6_DnaBD.
DR   PANTHER; PTHR46910:SF12; REGULATORY PROTEIN CAT8; 1.
DR   PANTHER; PTHR46910; TRANSCRIPTION FACTOR PDR1; 1.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; Zn2/Cys6 DNA-binding domain; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Carbohydrate metabolism; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..1433
FT                   /note="Regulatory protein CAT8"
FT                   /id="PRO_0000114940"
FT   DNA_BIND        70..97
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          20..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          936..1024
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1137..1162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1200..1236
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1324..1433
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..40
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        936..950
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        951..965
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        966..991
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        992..1009
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1324..1394
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1407..1433
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        303
FT                   /note="S -> A (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        747
FT                   /note="K -> L (in Ref. 1; CAA55139)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        768
FT                   /note="T -> A (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        999..1002
FT                   /note="Missing (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1008
FT                   /note="G -> S (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1016
FT                   /note="H -> Q (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1019
FT                   /note="Q -> P (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1061
FT                   /note="V -> M (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1072..1074
FT                   /note="TDA -> ADS (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1092
FT                   /note="N -> S (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1100
FT                   /note="I -> V (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1120
FT                   /note="M -> L (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1162
FT                   /note="S -> A (in Ref. 4; CAA63906)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1433 AA;  160485 MW;  5ED790BEFB47B632 CRC64;
     MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM IKTASPTPLS
     TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS DKLLRKAYPK GYTESLEERV
     RELEAENKRL LALCDIKEQQ ISLVSQSRPQ TSTDNTINGN FKHDLKDAPL NLSSTNIYLL
     NQTVNKQLQN GKMDGDNSGS AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT
     AISFEQDEAP GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG
     FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV KRFQSTNLSE
     FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK ELLHLFFKFW SNQVPILNND
     HFLIYFNNFV EVVKHLSTEN LETNNTTKST VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK
     YTVPKNPKAK YARLMAYYHQ LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA
     IYGVRGRIVS MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG
     VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL QIIRFAKILG
     NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY FQITVNGTVN LDEIRATNQR
     NTETKFDKKD IILFEKKILL LFYFLAKSMI HLPVIATKPL PKNVDNATKK KQSMFNNDSK
     GATNQDHMIL DVDMTSPAIR TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF
     SLLCARGSLE YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL
     KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR QRTPNVKREN
     PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA KRNAKDGGIS KGENAHNFQN
     DTKKNMSTSN LFPFSFSNTD LTALFTHPEG PNCTNTNNGN VDVCNRASTD ATDANIENLS
     FLNMAPFLQT GNSNIGQNTI ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE
     NSKNNQFHQK GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS
     ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG NFNNFMTNVN
     YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK NSIILDTTFN DDLDRSRMNA
     REVLNPTDSI LSQGMVSSVS TRNTSNQRSL SSGNDSKGDS SSQENSKSAT GNQLDTPSTL
     FQMRRTSSGP SASHRGPRRP QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK
//