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P39113

- CAT8_YEAST

UniProt

P39113 - CAT8_YEAST

Protein

Regulatory protein CAT8

Gene

CAT8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi70 – 9728Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Carbohydrate metabolism, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32951-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein CAT8
    Gene namesi
    Name:CAT8
    Synonyms:MSP8
    Ordered Locus Names:YMR280C
    ORF Names:YM8021.06C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR280c.
    SGDiS000004893. CAT8.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14331433Regulatory protein CAT8PRO_0000114940Add
    BLAST

    Post-translational modificationi

    Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.

    Proteomic databases

    MaxQBiP39113.
    PaxDbiP39113.
    PeptideAtlasiP39113.

    Expressioni

    Gene expression databases

    GenevestigatoriP39113.

    Interactioni

    Protein-protein interaction databases

    BioGridi35459. 26 interactions.
    DIPiDIP-4397N.
    IntActiP39113. 1 interaction.
    MINTiMINT-489548.
    STRINGi4932.YMR280C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39113.
    SMRiP39113. Positions 68-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi208 – 2125Poly-Pro
    Compositional biasi972 – 9765Poly-Asn

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG76428.
    GeneTreeiENSGT00530000068569.
    HOGENOMiHOG000111315.
    KOiK09240.
    OMAiLFWAIYY.
    OrthoDBiEOG7GTTDC.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view]
    SMARTiSM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39113-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM     50
    IKTASPTPLS TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS 100
    DKLLRKAYPK GYTESLEERV RELEAENKRL LALCDIKEQQ ISLVSQSRPQ 150
    TSTDNTINGN FKHDLKDAPL NLSSTNIYLL NQTVNKQLQN GKMDGDNSGS 200
    AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT AISFEQDEAP 250
    GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG 300
    FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV 350
    KRFQSTNLSE FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK 400
    ELLHLFFKFW SNQVPILNND HFLIYFNNFV EVVKHLSTEN LETNNTTKST 450
    VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK YTVPKNPKAK YARLMAYYHQ 500
    LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA IYGVRGRIVS 550
    MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG 600
    VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL 650
    QIIRFAKILG NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY 700
    FQITVNGTVN LDEIRATNQR NTETKFDKKD IILFEKKILL LFYFLAKSMI 750
    HLPVIATKPL PKNVDNATKK KQSMFNNDSK GATNQDHMIL DVDMTSPAIR 800
    TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF SLLCARGSLE 850
    YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL 900
    KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR 950
    QRTPNVKREN PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA 1000
    KRNAKDGGIS KGENAHNFQN DTKKNMSTSN LFPFSFSNTD LTALFTHPEG 1050
    PNCTNTNNGN VDVCNRASTD ATDANIENLS FLNMAPFLQT GNSNIGQNTI 1100
    ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE NSKNNQFHQK 1150
    GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS 1200
    ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG 1250
    NFNNFMTNVN YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK 1300
    NSIILDTTFN DDLDRSRMNA REVLNPTDSI LSQGMVSSVS TRNTSNQRSL 1350
    SSGNDSKGDS SSQENSKSAT GNQLDTPSTL FQMRRTSSGP SASHRGPRRP 1400
    QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK 1433
    Length:1,433
    Mass (Da):160,485
    Last modified:October 1, 1996 - v2
    Checksum:i5ED790BEFB47B632
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti303 – 3031S → A in CAA63906. 1 PublicationCurated
    Sequence conflicti747 – 7471K → L in CAA55139. (PubMed:7891685)Curated
    Sequence conflicti768 – 7681T → A in CAA63906. 1 PublicationCurated
    Sequence conflicti999 – 10024Missing in CAA63906. 1 PublicationCurated
    Sequence conflicti1008 – 10081G → S in CAA63906. 1 PublicationCurated
    Sequence conflicti1016 – 10161H → Q in CAA63906. 1 PublicationCurated
    Sequence conflicti1019 – 10191Q → P in CAA63906. 1 PublicationCurated
    Sequence conflicti1061 – 10611V → M in CAA63906. 1 PublicationCurated
    Sequence conflicti1072 – 10743TDA → ADS in CAA63906. 1 PublicationCurated
    Sequence conflicti1092 – 10921N → S in CAA63906. 1 PublicationCurated
    Sequence conflicti1100 – 11001I → V in CAA63906. 1 PublicationCurated
    Sequence conflicti1120 – 11201M → L in CAA63906. 1 PublicationCurated
    Sequence conflicti1162 – 11621S → A in CAA63906. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78344 Genomic DNA. Translation: CAA55139.1.
    Z49704 Genomic DNA. Translation: CAA89778.1.
    X94215 Genomic DNA. Translation: CAA63906.1.
    BK006946 Genomic DNA. Translation: DAA10181.1.
    PIRiS54587.
    RefSeqiNP_014007.1. NM_001182787.1.

    Genome annotation databases

    EnsemblFungiiYMR280C; YMR280C; YMR280C.
    GeneIDi855323.
    KEGGisce:YMR280C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78344 Genomic DNA. Translation: CAA55139.1 .
    Z49704 Genomic DNA. Translation: CAA89778.1 .
    X94215 Genomic DNA. Translation: CAA63906.1 .
    BK006946 Genomic DNA. Translation: DAA10181.1 .
    PIRi S54587.
    RefSeqi NP_014007.1. NM_001182787.1.

    3D structure databases

    ProteinModelPortali P39113.
    SMRi P39113. Positions 68-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35459. 26 interactions.
    DIPi DIP-4397N.
    IntActi P39113. 1 interaction.
    MINTi MINT-489548.
    STRINGi 4932.YMR280C.

    Proteomic databases

    MaxQBi P39113.
    PaxDbi P39113.
    PeptideAtlasi P39113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR280C ; YMR280C ; YMR280C .
    GeneIDi 855323.
    KEGGi sce:YMR280C.

    Organism-specific databases

    CYGDi YMR280c.
    SGDi S000004893. CAT8.

    Phylogenomic databases

    eggNOGi NOG76428.
    GeneTreei ENSGT00530000068569.
    HOGENOMi HOG000111315.
    KOi K09240.
    OMAi LFWAIYY.
    OrthoDBi EOG7GTTDC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32951-MONOMER.

    Miscellaneous databases

    NextBioi 979032.

    Gene expression databases

    Genevestigatori P39113.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    SMARTi SM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae."
      Hedges D., Proft M., Entian K.-D.
      Mol. Cell. Biol. 15:1915-1922(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Boles E., Hettmann C., Zimmermann F.K.
      Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ENY.WA-1A.
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCAT8_YEAST
    AccessioniPrimary (citable) accession number: P39113
    Secondary accession number(s): D6W0A7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3