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Protein

Regulatory protein CAT8

Gene

CAT8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi70 – 9728Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32951-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein CAT8
Gene namesi
Name:CAT8
Synonyms:MSP8
Ordered Locus Names:YMR280C
ORF Names:YM8021.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR280c.
SGDiS000004893. CAT8.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14331433Regulatory protein CAT8PRO_0000114940Add
BLAST

Post-translational modificationi

Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.

Proteomic databases

MaxQBiP39113.
PaxDbiP39113.
PeptideAtlasiP39113.

Expressioni

Gene expression databases

GenevestigatoriP39113.

Interactioni

Protein-protein interaction databases

BioGridi35459. 29 interactions.
DIPiDIP-4397N.
IntActiP39113. 1 interaction.
MINTiMINT-489548.
STRINGi4932.YMR280C.

Structurei

3D structure databases

ProteinModelPortaliP39113.
SMRiP39113. Positions 68-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi208 – 2125Poly-Pro
Compositional biasi972 – 9765Poly-Asn

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG76428.
GeneTreeiENSGT00530000068569.
HOGENOMiHOG000111315.
InParanoidiP39113.
KOiK09240.
OMAiLFWAIYY.
OrthoDBiEOG7GTTDC.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM
60 70 80 90 100
IKTASPTPLS TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS
110 120 130 140 150
DKLLRKAYPK GYTESLEERV RELEAENKRL LALCDIKEQQ ISLVSQSRPQ
160 170 180 190 200
TSTDNTINGN FKHDLKDAPL NLSSTNIYLL NQTVNKQLQN GKMDGDNSGS
210 220 230 240 250
AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT AISFEQDEAP
260 270 280 290 300
GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG
310 320 330 340 350
FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV
360 370 380 390 400
KRFQSTNLSE FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK
410 420 430 440 450
ELLHLFFKFW SNQVPILNND HFLIYFNNFV EVVKHLSTEN LETNNTTKST
460 470 480 490 500
VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK YTVPKNPKAK YARLMAYYHQ
510 520 530 540 550
LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA IYGVRGRIVS
560 570 580 590 600
MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG
610 620 630 640 650
VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL
660 670 680 690 700
QIIRFAKILG NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY
710 720 730 740 750
FQITVNGTVN LDEIRATNQR NTETKFDKKD IILFEKKILL LFYFLAKSMI
760 770 780 790 800
HLPVIATKPL PKNVDNATKK KQSMFNNDSK GATNQDHMIL DVDMTSPAIR
810 820 830 840 850
TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF SLLCARGSLE
860 870 880 890 900
YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL
910 920 930 940 950
KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR
960 970 980 990 1000
QRTPNVKREN PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA
1010 1020 1030 1040 1050
KRNAKDGGIS KGENAHNFQN DTKKNMSTSN LFPFSFSNTD LTALFTHPEG
1060 1070 1080 1090 1100
PNCTNTNNGN VDVCNRASTD ATDANIENLS FLNMAPFLQT GNSNIGQNTI
1110 1120 1130 1140 1150
ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE NSKNNQFHQK
1160 1170 1180 1190 1200
GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS
1210 1220 1230 1240 1250
ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG
1260 1270 1280 1290 1300
NFNNFMTNVN YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK
1310 1320 1330 1340 1350
NSIILDTTFN DDLDRSRMNA REVLNPTDSI LSQGMVSSVS TRNTSNQRSL
1360 1370 1380 1390 1400
SSGNDSKGDS SSQENSKSAT GNQLDTPSTL FQMRRTSSGP SASHRGPRRP
1410 1420 1430
QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK
Length:1,433
Mass (Da):160,485
Last modified:October 1, 1996 - v2
Checksum:i5ED790BEFB47B632
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3031S → A in CAA63906 (Ref. 4) Curated
Sequence conflicti747 – 7471K → L in CAA55139 (PubMed:7891685).Curated
Sequence conflicti768 – 7681T → A in CAA63906 (Ref. 4) Curated
Sequence conflicti999 – 10024Missing in CAA63906 (Ref. 4) Curated
Sequence conflicti1008 – 10081G → S in CAA63906 (Ref. 4) Curated
Sequence conflicti1016 – 10161H → Q in CAA63906 (Ref. 4) Curated
Sequence conflicti1019 – 10191Q → P in CAA63906 (Ref. 4) Curated
Sequence conflicti1061 – 10611V → M in CAA63906 (Ref. 4) Curated
Sequence conflicti1072 – 10743TDA → ADS in CAA63906 (Ref. 4) Curated
Sequence conflicti1092 – 10921N → S in CAA63906 (Ref. 4) Curated
Sequence conflicti1100 – 11001I → V in CAA63906 (Ref. 4) Curated
Sequence conflicti1120 – 11201M → L in CAA63906 (Ref. 4) Curated
Sequence conflicti1162 – 11621S → A in CAA63906 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78344 Genomic DNA. Translation: CAA55139.1.
Z49704 Genomic DNA. Translation: CAA89778.1.
X94215 Genomic DNA. Translation: CAA63906.1.
BK006946 Genomic DNA. Translation: DAA10181.1.
PIRiS54587.
RefSeqiNP_014007.1. NM_001182787.1.

Genome annotation databases

EnsemblFungiiYMR280C; YMR280C; YMR280C.
GeneIDi855323.
KEGGisce:YMR280C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78344 Genomic DNA. Translation: CAA55139.1.
Z49704 Genomic DNA. Translation: CAA89778.1.
X94215 Genomic DNA. Translation: CAA63906.1.
BK006946 Genomic DNA. Translation: DAA10181.1.
PIRiS54587.
RefSeqiNP_014007.1. NM_001182787.1.

3D structure databases

ProteinModelPortaliP39113.
SMRiP39113. Positions 68-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35459. 29 interactions.
DIPiDIP-4397N.
IntActiP39113. 1 interaction.
MINTiMINT-489548.
STRINGi4932.YMR280C.

Proteomic databases

MaxQBiP39113.
PaxDbiP39113.
PeptideAtlasiP39113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR280C; YMR280C; YMR280C.
GeneIDi855323.
KEGGisce:YMR280C.

Organism-specific databases

CYGDiYMR280c.
SGDiS000004893. CAT8.

Phylogenomic databases

eggNOGiNOG76428.
GeneTreeiENSGT00530000068569.
HOGENOMiHOG000111315.
InParanoidiP39113.
KOiK09240.
OMAiLFWAIYY.
OrthoDBiEOG7GTTDC.

Enzyme and pathway databases

BioCyciYEAST:G3O-32951-MONOMER.

Miscellaneous databases

NextBioi979032.

Gene expression databases

GenevestigatoriP39113.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae."
    Hedges D., Proft M., Entian K.-D.
    Mol. Cell. Biol. 15:1915-1922(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Boles E., Hettmann C., Zimmermann F.K.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ENY.WA-1A.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAT8_YEAST
AccessioniPrimary (citable) accession number: P39113
Secondary accession number(s): D6W0A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.