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P39113 (CAT8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein CAT8
Gene names
Name:CAT8
Synonyms:MSP8
Ordered Locus Names:YMR280C
ORF Names:YM8021.06C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1433 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.

Subcellular location

Nucleus.

Post-translational modification

Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 11024040Ref.1PubMed 8710504. Source: SGD

positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 11024040PubMed 8710504. Source: SGD

positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source

Inferred from mutant phenotype PubMed 10540293PubMed 10628872PubMed 11024040Ref.1PubMed 8710504PubMed 9894915. Source: SGD

   Cellular_componentnucleus

Inferred by curator PubMed 14685767. Source: SGD

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 10540293PubMed 11405098PubMed 14685767. Source: SGD

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 14685767. Source: SGD

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14331433Regulatory protein CAT8
PRO_0000114940

Regions

DNA binding70 – 9728Zn(2)-C6 fungal-type
Compositional bias208 – 2125Poly-Pro
Compositional bias972 – 9765Poly-Asn

Experimental info

Sequence conflict3031S → A in CAA63906. Ref.4
Sequence conflict7471K → L in CAA55139. Ref.1
Sequence conflict7681T → A in CAA63906. Ref.4
Sequence conflict999 – 10024Missing in CAA63906. Ref.4
Sequence conflict10081G → S in CAA63906. Ref.4
Sequence conflict10161H → Q in CAA63906. Ref.4
Sequence conflict10191Q → P in CAA63906. Ref.4
Sequence conflict10611V → M in CAA63906. Ref.4
Sequence conflict1072 – 10743TDA → ADS in CAA63906. Ref.4
Sequence conflict10921N → S in CAA63906. Ref.4
Sequence conflict11001I → V in CAA63906. Ref.4
Sequence conflict11201M → L in CAA63906. Ref.4
Sequence conflict11621S → A in CAA63906. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P39113 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 5ED790BEFB47B632

FASTA1,433160,485
        10         20         30         40         50         60 
MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM IKTASPTPLS 

        70         80         90        100        110        120 
TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS DKLLRKAYPK GYTESLEERV 

       130        140        150        160        170        180 
RELEAENKRL LALCDIKEQQ ISLVSQSRPQ TSTDNTINGN FKHDLKDAPL NLSSTNIYLL 

       190        200        210        220        230        240 
NQTVNKQLQN GKMDGDNSGS AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT 

       250        260        270        280        290        300 
AISFEQDEAP GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG 

       310        320        330        340        350        360 
FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV KRFQSTNLSE 

       370        380        390        400        410        420 
FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK ELLHLFFKFW SNQVPILNND 

       430        440        450        460        470        480 
HFLIYFNNFV EVVKHLSTEN LETNNTTKST VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK 

       490        500        510        520        530        540 
YTVPKNPKAK YARLMAYYHQ LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA 

       550        560        570        580        590        600 
IYGVRGRIVS MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG 

       610        620        630        640        650        660 
VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL QIIRFAKILG 

       670        680        690        700        710        720 
NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY FQITVNGTVN LDEIRATNQR 

       730        740        750        760        770        780 
NTETKFDKKD IILFEKKILL LFYFLAKSMI HLPVIATKPL PKNVDNATKK KQSMFNNDSK 

       790        800        810        820        830        840 
GATNQDHMIL DVDMTSPAIR TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF 

       850        860        870        880        890        900 
SLLCARGSLE YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL 

       910        920        930        940        950        960 
KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR QRTPNVKREN 

       970        980        990       1000       1010       1020 
PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA KRNAKDGGIS KGENAHNFQN 

      1030       1040       1050       1060       1070       1080 
DTKKNMSTSN LFPFSFSNTD LTALFTHPEG PNCTNTNNGN VDVCNRASTD ATDANIENLS 

      1090       1100       1110       1120       1130       1140 
FLNMAPFLQT GNSNIGQNTI ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE 

      1150       1160       1170       1180       1190       1200 
NSKNNQFHQK GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS 

      1210       1220       1230       1240       1250       1260 
ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG NFNNFMTNVN 

      1270       1280       1290       1300       1310       1320 
YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK NSIILDTTFN DDLDRSRMNA 

      1330       1340       1350       1360       1370       1380 
REVLNPTDSI LSQGMVSSVS TRNTSNQRSL SSGNDSKGDS SSQENSKSAT GNQLDTPSTL 

      1390       1400       1410       1420       1430 
FQMRRTSSGP SASHRGPRRP QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK 

« Hide

References

« Hide 'large scale' references
[1]"CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae."
Hedges D., Proft M., Entian K.-D.
Mol. Cell. Biol. 15:1915-1922(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]Boles E., Hettmann C., Zimmermann F.K.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ENY.WA-1A.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78344 Genomic DNA. Translation: CAA55139.1.
Z49704 Genomic DNA. Translation: CAA89778.1.
X94215 Genomic DNA. Translation: CAA63906.1.
BK006946 Genomic DNA. Translation: DAA10181.1.
PIRS54587.
RefSeqNP_014007.1. NM_001182787.1.

3D structure databases

ProteinModelPortalP39113.
SMRP39113. Positions 62-124.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35459. 26 interactions.
DIPDIP-4397N.
IntActP39113. 1 interaction.
MINTMINT-489548.
STRING4932.YMR280C.

Proteomic databases

PaxDbP39113.
PeptideAtlasP39113.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR280C; YMR280C; YMR280C.
GeneID855323.
KEGGsce:YMR280C.

Organism-specific databases

CYGDYMR280c.
SGDS000004893. CAT8.

Phylogenomic databases

eggNOGNOG76428.
GeneTreeENSGT00530000068569.
HOGENOMHOG000111315.
KOK09240.
OMALFWAIYY.
OrthoDBEOG7GTTDC.

Enzyme and pathway databases

BioCycYEAST:G3O-32951-MONOMER.

Gene expression databases

GenevestigatorP39113.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979032.

Entry information

Entry nameCAT8_YEAST
AccessionPrimary (citable) accession number: P39113
Secondary accession number(s): D6W0A7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families