P39113 (CAT8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein CAT8

Gene names

Name:	CAT8
Synonyms:	MSP8
Ordered Locus Names:	YMR280C
ORF Names:	YM8021.06C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.
Subcellular location	Nucleus.
Post-translational modification	Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11024040Ref.1PubMed 8710504. Source: SGD positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11024040PubMed 8710504. Source: SGD positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Inferred from mutant phenotype PubMed 10540293PubMed 10628872PubMed 11024040Ref.1PubMed 8710504PubMed 9894915. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD nucleus Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 10540293PubMed 11405098PubMed 14685767. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 14685767. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1433	1433	Regulatory protein CAT8		PRO_0000114940
Regions
	DNA binding	70 – 97	28	Zn(2)-C6 fungal-type
	Compositional bias	208 – 212	5	Poly-Pro
	Compositional bias	972 – 976	5	Poly-Asn
Amino acid modifications
	Modified residue	173	1	Phosphoserine Ref.5
	Modified residue	175	1	Phosphothreonine Ref.5
	Modified residue	1170	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	303	1	S → A in CAA63906. Ref.4
	Sequence conflict	747	1	K → L in CAA55139. Ref.1
	Sequence conflict	768	1	T → A in CAA63906. Ref.4
	Sequence conflict	999 – 1002	4	Missing in CAA63906. Ref.4
	Sequence conflict	1008	1	G → S in CAA63906. Ref.4
	Sequence conflict	1016	1	H → Q in CAA63906. Ref.4
	Sequence conflict	1019	1	Q → P in CAA63906. Ref.4
	Sequence conflict	1061	1	V → M in CAA63906. Ref.4
	Sequence conflict	1072 – 1074	3	TDA → ADS in CAA63906. Ref.4
	Sequence conflict	1092	1	N → S in CAA63906. Ref.4
	Sequence conflict	1100	1	I → V in CAA63906. Ref.4
	Sequence conflict	1120	1	M → L in CAA63906. Ref.4
	Sequence conflict	1162	1	S → A in CAA63906. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39113 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 5ED790BEFB47B632	FASTA	1,433	160,485
	10 20 30 40 50 60 MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM IKTASPTPLS 70 80 90 100 110 120 TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS DKLLRKAYPK GYTESLEERV 130 140 150 160 170 180 RELEAENKRL LALCDIKEQQ ISLVSQSRPQ TSTDNTINGN FKHDLKDAPL NLSSTNIYLL 190 200 210 220 230 240 NQTVNKQLQN GKMDGDNSGS AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT 250 260 270 280 290 300 AISFEQDEAP GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG 310 320 330 340 350 360 FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV KRFQSTNLSE 370 380 390 400 410 420 FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK ELLHLFFKFW SNQVPILNND 430 440 450 460 470 480 HFLIYFNNFV EVVKHLSTEN LETNNTTKST VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK 490 500 510 520 530 540 YTVPKNPKAK YARLMAYYHQ LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA 550 560 570 580 590 600 IYGVRGRIVS MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG 610 620 630 640 650 660 VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL QIIRFAKILG 670 680 690 700 710 720 NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY FQITVNGTVN LDEIRATNQR 730 740 750 760 770 780 NTETKFDKKD IILFEKKILL LFYFLAKSMI HLPVIATKPL PKNVDNATKK KQSMFNNDSK 790 800 810 820 830 840 GATNQDHMIL DVDMTSPAIR TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF 850 860 870 880 890 900 SLLCARGSLE YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL 910 920 930 940 950 960 KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR QRTPNVKREN 970 980 990 1000 1010 1020 PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA KRNAKDGGIS KGENAHNFQN 1030 1040 1050 1060 1070 1080 DTKKNMSTSN LFPFSFSNTD LTALFTHPEG PNCTNTNNGN VDVCNRASTD ATDANIENLS 1090 1100 1110 1120 1130 1140 FLNMAPFLQT GNSNIGQNTI ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE 1150 1160 1170 1180 1190 1200 NSKNNQFHQK GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS 1210 1220 1230 1240 1250 1260 ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG NFNNFMTNVN 1270 1280 1290 1300 1310 1320 YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK NSIILDTTFN DDLDRSRMNA 1330 1340 1350 1360 1370 1380 REVLNPTDSI LSQGMVSSVS TRNTSNQRSL SSGNDSKGDS SSQENSKSAT GNQLDTPSTL 1390 1400 1410 1420 1430 FQMRRTSSGP SASHRGPRRP QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae." Hedges D., Proft M., Entian K.-D. Mol. Cell. Biol. 15:1915-1922(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	Boles E., Hettmann C., Zimmermann F.K. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ENY.WA-1A.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173; THR-175 AND SER-1170, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78344 Genomic DNA. Translation: CAA55139.1. Z49704 Genomic DNA. Translation: CAA89778.1. X94215 Genomic DNA. Translation: CAA63906.1. BK006946 Genomic DNA. Translation: DAA10181.1.
PIR	S54587.
RefSeq	NP_014007.1. NM_001182787.1.
3D structure databases
ProteinModelPortal	P39113.
SMR	P39113. Positions 68-124.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4397N.
IntAct	P39113. 1 interaction.
MINT	MINT-489548.
STRING	4932.YMR280C.
Proteomic databases
PaxDb	P39113.
PeptideAtlas	P39113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR280C; YMR280C; YMR280C.
GeneID	855323.
KEGG	sce:YMR280C.
Organism-specific databases
CYGD	YMR280c.
SGD	S000004893. CAT8.
Phylogenomic databases
eggNOG	NOG76428.
GeneTree	ENSGT00530000068569.
HOGENOM	HOG000111315.
KO	K09240.
OMA	MIHLPVI.
OrthoDB	EOG40S3Q0.
Gene expression databases
Genevestigator	P39113.
GermOnline	YMR280C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR007219. Transcription_factor_fun. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Pfam	PF04082. Fungal_trans. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00906. Fungal_trans. 1 hit. SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979032.

Entry information

Entry name

CAT8_YEAST

Accession

Primary (citable) accession number: P39113
Secondary accession number(s): D6W0A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program