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Protein

Exoribonuclease II, mitochondrial

Gene

DSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for mitochondrial biogenesis.
Required for intron-independent turnover and processing of mitochondrial RNA. Participates in 3' mtRNA processing where it hydrolyzes single-stranded RNA or partially double-stranded RNA with 3' single-stranded tails.

Miscellaneous

Present with 1070 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: GO_Central
  • exoribonuclease II activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mitochondrial RNA catabolic process Source: SGD
  • rRNA processing Source: GO_Central

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32957-MONOMER.
BRENDAi3.1.13.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoribonuclease II, mitochondrial (EC:3.1.13.1)
Short name:
RNase II
Short name:
Ribonuclease II
Alternative name(s):
Deletion of SUV3 suppressor 1
Mitochondrial biogenesis protein MSU1
Gene namesi
Name:DSS1
Synonyms:MSU1
Ordered Locus Names:YMR287C
ORF Names:YM8021.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR287C.
SGDiS000004900. DSS1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41Mitochondrion1 PublicationAdd BLAST41
ChainiPRO_000003082042 – 969Exoribonuclease II, mitochondrialAdd BLAST928

Proteomic databases

MaxQBiP39112.
PRIDEiP39112.

PTM databases

iPTMnetiP39112.

Interactioni

Subunit structurei

MSU1 and SUV3 are the two components of the mitochondrial degradosome (mtEXO).1 Publication

Protein-protein interaction databases

BioGridi35467. 87 interactors.
DIPiDIP-2936N.
IntActiP39112. 6 interactors.
MINTiMINT-547765.
STRINGi4932.YMR287C.

Structurei

3D structure databases

ProteinModelPortaliP39112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNR ribonuclease family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiP39112.
KOiK19782.
OMAiSETAHEI.
OrthoDBiEOG092C0LRK.

Family and domain databases

InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR022966. RNase_II/R_CS.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiView protein in PROSITE
PS01175. RIBONUCLEASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVRRKVHVL LIARSFHSYT PCFRVTTRGK RQRSKSKQQA KVELDHTREL
60 70 80 90 100
DNDQATETVV DRSVGPEKDI ESINKDFLQR TKGLEPDIEL KQLPQIKQEF
110 120 130 140 150
NQRYKDRYVK PSEDWYVNSW RSLTKPKIPL YKLINSDFQL ITKLKAPNPM
160 170 180 190 200
EFQPVQLMES PLNVGDFVLL KMRPNELAMC VSLPSSTMDP RYTFVTIDGT
210 220 230 240 250
MCFATKNRVL LRIPHKLPAG IHSLIQPESH HKHLPIGTVK NFSNQTNILP
260 270 280 290 300
IVARQLITSR YPAQISKLAW KDLPITTKKL QLLHRSLQNY MGPWQIPFFT
310 320 330 340 350
LVGLVQKLDL NKALDDKNGI NYLTSLVNNY HTVNDIPINS PTFVSTYWAI
360 370 380 390 400
MQQQESNLWG EIHLNTALLS PISVTIIPLK SQHLYYAQVI EKLEANSYRE
410 420 430 440 450
VNKFVKLVNE RKYRDISALY PSVIQLLKDF AAGNFHNNGI IVALISKIFR
460 470 480 490 500
KIERYKDCDI TRDICQDLIN EITPNSIPNP LLLNMDLALP ASSKLVKWQQ
510 520 530 540 550
KLYDLTNIEE LQWKKSGTDD DRYDFGDLRV FCIDSETAHE IDDGVSVKNY
560 570 580 590 600
GRDGLYTLYI HIADPTSMFP ESTNVDIEGI STDILNVALK RSFTTYLPDT
610 620 630 640 650
VVPMLPQSIC HLSDLGKQGQ RTKTISFSVD VKITSKCSGK SIEIMYDSFK
660 670 680 690 700
IRKGIVSNFP KATYEDVDRI LGTPNSEASP VKKDLESLSM ISKLLREQRI
710 720 730 740 750
KNSNAVIFGE GFNKGLVMLN ADSEGELTEV TFSDQEETLS TILVSEMMIL
760 770 780 790 800
ANTLTGRYFA ENKIGGVFRC YKQLPLGEVA QQQYDSMITS TKKGIFPKLK
810 820 830 840 850
DIVKLSSLLN SSFYTGRPFR HEMIGAKQYL TVTSPLRRFP DLINHLQIHR
860 870 880 890 900
HLQKKPLCFN QTQIDSLIWP IQSRADILKR ASRNSSTYWT LNYLKKLTKL
910 920 930 940 950
EPERTFDVMV TSVPQNGFTG CVFPDLSFAR GTLKLHPSSM HYPMIGDIVK
960
NCKISKIDCL EGMLELEKL
Length:969
Mass (Da):110,822
Last modified:October 1, 1996 - v2
Checksum:iE8D898F2DB4EFA2D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222H → R in AAC49144 (PubMed:8590460).Curated1
Sequence conflicti870P → T in D90217 (PubMed:2061283).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15461 Genomic DNA. Translation: AAC49144.1.
Z49704 Genomic DNA. Translation: CAA89785.1.
D90217 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA10188.1.
PIRiS54594.
RefSeqiNP_014014.1. NM_001182794.1.

Genome annotation databases

EnsemblFungiiYMR287C; YMR287C; YMR287C.
GeneIDi855331.
KEGGisce:YMR287C.

Similar proteinsi

Entry informationi

Entry nameiDSS1_YEAST
AccessioniPrimary (citable) accession number: P39112
Secondary accession number(s): D6W0B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names