ID   CIN4_YEAST              Reviewed;         191 AA.
AC   P39110; D6VZW1;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 178.
DE   RecName: Full=GTP-binding protein CIN4;
DE   AltName: Full=Chromosome instability protein 4;
GN   Name=CIN4; Synonyms=GTP1, UGX1; OrderedLocusNames=YMR138W;
GN   ORFNames=YM9375.07;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RA   Stearns T., Clark C.D., Hoyt M.A., Kahn R.A., Botstein D.;
RT   "A small GTP-binding protein implicated in yeast microtubule function.";
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7874754; DOI=10.1007/bf00309951;
RA   Wolter R., Richter D., Niegemann E., Brendel M.;
RT   "Molecular characterisation of GTP1, a Saccharomyces cerevisiae gene
RT   encoding a small GTP-binding protein.";
RL   Curr. Genet. 26:564-566(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Implicated in yeast microtubule function.
CC   -!- MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; L36669; AAA50350.1; -; Genomic_DNA.
DR   EMBL; X77086; CAA54358.1; -; Genomic_DNA.
DR   EMBL; Z47071; CAA87352.1; -; Genomic_DNA.
DR   EMBL; AY558409; AAS56735.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10035.1; -; Genomic_DNA.
DR   PIR; S51496; S51496.
DR   RefSeq; NP_013858.1; NM_001182640.1.
DR   AlphaFoldDB; P39110; -.
DR   SMR; P39110; -.
DR   BioGRID; 35315; 119.
DR   DIP; DIP-1256N; -.
DR   IntAct; P39110; 7.
DR   MINT; P39110; -.
DR   STRING; 4932.YMR138W; -.
DR   MaxQB; P39110; -.
DR   PaxDb; 4932-YMR138W; -.
DR   PeptideAtlas; P39110; -.
DR   EnsemblFungi; YMR138W_mRNA; YMR138W; YMR138W.
DR   GeneID; 855169; -.
DR   KEGG; sce:YMR138W; -.
DR   AGR; SGD:S000004746; -.
DR   SGD; S000004746; CIN4.
DR   VEuPathDB; FungiDB:YMR138W; -.
DR   eggNOG; KOG0073; Eukaryota.
DR   GeneTree; ENSGT00940000157941; -.
DR   HOGENOM; CLU_040729_12_3_1; -.
DR   InParanoid; P39110; -.
DR   OMA; MNIDRHW; -.
DR   OrthoDB; 5349301at2759; -.
DR   BioCyc; YEAST:G3O-32831-MONOMER; -.
DR   BioGRID-ORCS; 855169; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P39110; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; P39110; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0015630; C:microtubule cytoskeleton; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006457; P:protein folding; IGI:SGD.
DR   GO; GO:0007021; P:tubulin complex assembly; IMP:SGD.
DR   CDD; cd00878; Arf_Arl; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR044612; ARL2/3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR   NCBIfam; TIGR00231; small_GTP; 1.
DR   PANTHER; PTHR45697:SF2; ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2; 1.
DR   PANTHER; PTHR45697; ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED; 1.
DR   Pfam; PF00025; Arf; 1.
DR   PRINTS; PR00328; SAR1GTPBP.
DR   SMART; SM00177; ARF; 1.
DR   SMART; SM00178; SAR; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51417; ARF; 1.
PE   1: Evidence at protein level;
KW   GTP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..191
FT                   /note="GTP-binding protein CIN4"
FT                   /id="PRO_0000122464"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         69..73
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         131..134
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   191 AA;  22067 MW;  CF30AF932A2D2566 CRC64;
     MGLLSIIRKQ KLRDKEIRCL ILGLDNSGKS TIVNKLLPKD EQNNDGIMPT VGFQIHSLMI
     KDVTISLWDI GGQRTLRPFW DNYFDKTQAM IWCIDVSLSM RFDETLQELK ELINRDENRI
     GYECAVIVVL NKIDLVEDKS ELHRRCLLVE SELKCLFKPD IRIELVKCSG VTGEGIDNLR
     DRLVESCHFT Q
//