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Protein

Metal resistance protein YCF1

Gene

YCF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi663 – 670ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1306 – 1313ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • bilirubin transmembrane transporter activity Source: SGD
  • glutathione S-conjugate-exporting ATPase activity Source: SGD

GO - Biological processi

  • bilirubin transport Source: SGD
  • cell redox homeostasis Source: SGD
  • glutathione metabolic process Source: SGD
  • response to cadmium ion Source: UniProtKB-KW
  • response to metal ion Source: SGD
  • transmembrane transport Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cadmium resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29733-MONOMER.
ReactomeiR-SCE-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-SCE-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-SCE-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.208.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Metal resistance protein YCF1
Alternative name(s):
Yeast cadmium factor 1
Gene namesi
Name:YCF1
Ordered Locus Names:YDR135C
ORF Names:YD9302.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR135C.
SGDiS000002542. YCF1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32VacuolarSequence analysisAdd BLAST32
Transmembranei33 – 53Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini54 – 73CytoplasmicSequence analysisAdd BLAST20
Transmembranei74 – 94Helical; Name=2PROSITE-ProRule annotationAdd BLAST21
Topological domaini95 – 99VacuolarSequence analysis5
Transmembranei100 – 120Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini121 – 130CytoplasmicSequence analysis10
Transmembranei131 – 151Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini152 – 169VacuolarSequence analysisAdd BLAST18
Transmembranei170 – 190Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini191 – 278CytoplasmicSequence analysisAdd BLAST88
Transmembranei279 – 299Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini300 – 345VacuolarSequence analysisAdd BLAST46
Transmembranei346 – 366Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini367 – 422CytoplasmicSequence analysisAdd BLAST56
Transmembranei423 – 443Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini444 – 446VacuolarSequence analysis3
Transmembranei447 – 467Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini468 – 530CytoplasmicSequence analysisAdd BLAST63
Transmembranei531 – 551Helical; Name=10PROSITE-ProRule annotationAdd BLAST21
Topological domaini552 – 572VacuolarSequence analysisAdd BLAST21
Transmembranei573 – 593Helical; Name=11PROSITE-ProRule annotationAdd BLAST21
Topological domaini594 – 943CytoplasmicSequence analysisAdd BLAST350
Transmembranei944 – 964Helical; Name=12PROSITE-ProRule annotationAdd BLAST21
Topological domaini965 – 1001VacuolarSequence analysisAdd BLAST37
Transmembranei1002 – 1023Helical; Name=13PROSITE-ProRule annotationAdd BLAST22
Topological domaini1024 – 1066CytoplasmicSequence analysisAdd BLAST43
Transmembranei1067 – 1087Helical; Name=14PROSITE-ProRule annotationAdd BLAST21
Topological domaini1088VacuolarSequence analysis1
Transmembranei1089 – 1109Helical; Name=15PROSITE-ProRule annotationAdd BLAST21
Topological domaini1110 – 1180CytoplasmicSequence analysisAdd BLAST71
Transmembranei1181 – 1201Helical; Name=16PROSITE-ProRule annotationAdd BLAST21
Topological domaini1202 – 1205VacuolarSequence analysis4
Transmembranei1206 – 1226Helical; Name=17PROSITE-ProRule annotationAdd BLAST21
Topological domaini1227 – 1515CytoplasmicSequence analysisAdd BLAST289

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi713Missing : Loss of function. 1
Mutagenesisi908S → A: Loss of function. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000934491 – 1515Metal resistance protein YCF1Add BLAST1515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineCombined sources1
Modified residuei873PhosphoserineCombined sources1
Modified residuei903PhosphoserineCombined sources1
Modified residuei908PhosphoserineCombined sources1
Modified residuei911PhosphothreonineCombined sources1
Modified residuei914PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39109.
PRIDEiP39109.

PTM databases

iPTMnetiP39109.

Interactioni

Protein-protein interaction databases

BioGridi32190. 62 interactors.
DIPiDIP-2612N.
IntActiP39109. 4 interactors.
MINTiMINT-424452.

Structurei

3D structure databases

ProteinModelPortaliP39109.
SMRiP39109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini287 – 590ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST304
Domaini626 – 853ABC transporter 1PROSITE-ProRule annotationAdd BLAST228
Domaini951 – 1235ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST285
Domaini1272 – 1507ABC transporter 2PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133687.
InParanoidiP39109.
OMAiNRHEDKE.
OrthoDBiEOG092C0QQU.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI
60 70 80 90 100
RDLVNLCKKK HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN
110 120 130 140 150
FTIVSQYAST MLSLFVALAL HWIEYDRSVV ANTVLLFYWL FETFGNFAKL
160 170 180 190 200
INILIRHTYE GIWYSGQTGF ILTLFQVITC ASILLLEALP KKPLMPHQHI
210 220 230 240 250
HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE ADLYKLPRNF
260 270 280 290 300
SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL
310 320 330 340 350
AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF
360 370 380 390 400
AMFLVGFTQT SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS
410 420 430 440 450
STGDIVNLMS VDVQKLQDLT QWLNLIWSGP FQIIICLYSL YKLLGNSMWV
460 470 480 490 500
GVIILVIMMP LNSFLMRIQK KLQKSQMKYK DERTRVISEI LNNIKSLKLY
510 520 530 540 550
AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP FLVSCCTFAV
560 570 580 590 600
FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT
610 620 630 640 650
FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN
660 670 680 690 700
FQAKKGNLTC IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP
710 720 730 740 750
WIMNGTVKEN ILFGHRYDAE FYEKTIKACA LTIDLAILMD GDKTLVGEKG
760 770 780 790 800
ISLSGGQKAR LSLARAVYAR ADTYLLDDPL AAVDEHVARH LIEHVLGPNG
810 820 830 840 850
LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI TKDADSPLWK
860 870 880 890 900
LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD
910 920 930 940 950
AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS
960 970 980 990 1000
VCVFILFIVI SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI
1010 1020 1030 1040 1050
GSALATLIQT IVLWVFCTIH ASKYLHNLMT NSVLRAPMTF FETTPIGRIL
1060 1070 1080 1090 1100
NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF TITVICATTW QFIFIIIPLS
1110 1120 1130 1140 1150
VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA TVRGYSQQKR
1160 1170 1180 1190 1200
FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK
1210 1220 1230 1240 1250
QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK
1260 1270 1280 1290 1300
SEAPLIVEGH RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE
1310 1320 1330 1340 1350
KVGIVGRTGA GKSSLTLALF RMIEASEGNI VIDNIAINEI GLYDLRHKLS
1360 1370 1380 1390 1400
IIPQDSQVFE GTVRENIDPI NQYTDEAIWR ALELSHLKEH VLSMSNDGLD
1410 1420 1430 1440 1450
AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV DVETDKVVQE
1460 1470 1480 1490 1500
TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS
1510
LFYSLCMEAG LVNEN
Length:1,515
Mass (Da):171,121
Last modified:May 30, 2000 - v2
Checksum:i30F92FDDBAF60431
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti680L → R in AAA50353 (PubMed:7521334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35237 Genomic DNA. Translation: AAA50353.1.
Z48179 Genomic DNA. Translation: CAA88217.1.
BK006938 Genomic DNA. Translation: DAA11979.1.
PIRiS51863.
RefSeqiNP_010419.3. NM_001180442.3.

Genome annotation databases

EnsemblFungiiYDR135C; YDR135C; YDR135C.
GeneIDi851713.
KEGGisce:YDR135C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35237 Genomic DNA. Translation: AAA50353.1.
Z48179 Genomic DNA. Translation: CAA88217.1.
BK006938 Genomic DNA. Translation: DAA11979.1.
PIRiS51863.
RefSeqiNP_010419.3. NM_001180442.3.

3D structure databases

ProteinModelPortaliP39109.
SMRiP39109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32190. 62 interactors.
DIPiDIP-2612N.
IntActiP39109. 4 interactors.
MINTiMINT-424452.

Protein family/group databases

TCDBi3.A.1.208.11. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiP39109.

Proteomic databases

MaxQBiP39109.
PRIDEiP39109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR135C; YDR135C; YDR135C.
GeneIDi851713.
KEGGisce:YDR135C.

Organism-specific databases

EuPathDBiFungiDB:YDR135C.
SGDiS000002542. YCF1.

Phylogenomic databases

GeneTreeiENSGT00860000133687.
InParanoidiP39109.
OMAiNRHEDKE.
OrthoDBiEOG092C0QQU.

Enzyme and pathway databases

BioCyciYEAST:G3O-29733-MONOMER.
ReactomeiR-SCE-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-SCE-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-SCE-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiP39109.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYCFI_YEAST
AccessioniPrimary (citable) accession number: P39109
Secondary accession number(s): D6VSB9, Q03905
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 175 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.