P39107 (MNN9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan polymerase complexes subunit MNN9
Short name=M-pol I subunit MNN9

Alternative name(s):
M-Pol II subunit MNN9

Gene names

Name:	MNN9
Ordered Locus Names:	YPL050C
ORF Names:	P7102.01

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex. Ref.7
Pathway	Protein modification; protein glycosylation.
Subunit structure	The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.4 Ref.6
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note: Cis-Golgi. Recycles between endoplasmic reticulum and Golgi. Ref.4 Ref.5 Ref.6
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ANP1/MMN9/VAN1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	barrier septum assembly Inferred from mutant phenotype. Source: SGD cell wall mannoprotein biosynthetic process Traceable author statement Ref.6. Source: UniProtKB protein N-linked glycosylation Inferred from direct assay. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell alpha-1,6-mannosyltransferase complex Inferred from physical interaction Ref.6. Source: SGD cis-Golgi network Traceable author statement. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannosyltransferase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANP1	P32629	6	EBI-11082,EBI-2595
VAN1	P23642	5	EBI-11082,EBI-20237

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 395	394	Mannan polymerase complexes subunit MNN9		PRO_0000193670
Regions
	Topological domain	2 – 17	16	Cytoplasmic Potential
	Transmembrane	18 – 33	16	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 395	362	Lumenal Potential
Experimental info
	Mutagenesis	236	1	D → A: Reduced activity of the M-Pol I complex. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P39107 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 461783BB4D700987	FASTA	395	45,955
	10 20 30 40 50 60 MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH KWAHEKENTF 70 80 90 100 110 120 YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD LNKLHSTSEA AVNKEHILIL 130 140 150 160 170 180 TPMQTFHQQY WDNLLQLNYP RELIELGFIT PRTATGDLAL KKLENAIKKV QTDKKTQRFS 190 200 210 220 230 240 KITILRQNSQ SFDKLMEKER HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII 250 260 270 280 290 300 ETPPSLIQDM TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD 310 320 330 340 350 360 EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD GAMFPNFPFY 370 380 390 HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins." Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L. Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed: 8146181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus." Hashimoto H., Yoda K. Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed: 9434768] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, SUBCELLULAR LOCATION, SUBUNIT.
[5]	"Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum." Todorow Z., Spang A., Carmack E., Yates J., Schekman R. Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed: 11095735] [Abstract] Cited for: PROTEIN SEQUENCE OF 143-153, SUBCELLULAR LOCATION. Strain: RSY455.
[6]	"Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity." Jungmann J., Munro S. EMBO J. 17:423-434(1998) [PubMed: 9430634] [Abstract] Cited for: ACTIVITY OF M-POL COMPLEXES, SUBUNIT, SUBCELLULAR LOCATION.
[7]	"The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis." Stolz J., Munro S. J. Biol. Chem. 277:44801-44808(2002) [PubMed: 12235155] [Abstract] Cited for: FUNCTION, COMPOSITION OF THE M-POL I COMPLEX, MUTAGENESIS OF ASP-236.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23752 Genomic DNA. Translation: AAA53677.1. U44030 Genomic DNA. Translation: AAB68171.1. BK006949 Genomic DNA. Translation: DAA11380.1.
PIR	S43746.
RefSeq	NP_015275.1. NM_001183864.1.
3D structure databases
ProteinModelPortal	P39107.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-783N.
IntAct	P39107. 22 interactions.
MINT	MINT-552187.
STRING	P39107.
Protein family/group databases
CAZy	GT62. Glycosyltransferase Family 62.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL050C; YPL050C; YPL050C.
GeneID	856057.
KEGG	sce:YPL050C.
NMPDR	fig|4932.3.peg.6408.
Organism-specific databases
SGD	S000005971. MNN9.
Phylogenomic databases
eggNOG	fuNOG05503.
GeneTree	EFGT00050000002721.
HOGENOM	HBG396793.
OMA	GLPNYLV.
OrthoDB	EOG4J9R7K.
Gene expression databases
ArrayExpress	P39107.
Genevestigator	P39107.
GermOnline	YPL050C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005109. Anp1. [Graphical view]
KO	K05529.
Pfam	PF03452. Anp1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981026.

Entry information

Entry name

MNN9_YEAST

Accession

Primary (citable) accession number: P39107
Secondary accession number(s): D6W3W4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program