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Protein

Mannan polymerase complexes subunit MNN9

Gene

MNN9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: UniProtKB
  • mannosylation Source: GOC
  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33963-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan polymerase complexes subunit MNN9
Short name:
M-pol I subunit MNN9
Alternative name(s):
M-Pol II subunit MNN9
Gene namesi
Name:MNN9
Ordered Locus Names:YPL050C
ORF Names:P7102.01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL050C.
SGDiS000005971. MNN9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 17CytoplasmicSequence analysisAdd BLAST16
Transmembranei18 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini34 – 395LumenalSequence analysisAdd BLAST362

GO - Cellular componenti

  • alpha-1,6-mannosyltransferase complex Source: SGD
  • cis-Golgi network Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi cis cisterna Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi236D → A: Reduced activity of the M-Pol I complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001936702 – 395Mannan polymerase complexes subunit MNN9Add BLAST394

Proteomic databases

MaxQBiP39107.
PRIDEiP39107.

Interactioni

Subunit structurei

The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANP1P326296EBI-11082,EBI-2595
VAN1P236423EBI-11082,EBI-20237

Protein-protein interaction databases

BioGridi36130. 31 interactors.
DIPiDIP-783N.
IntActiP39107. 21 interactors.
MINTiMINT-552187.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi101 – 103Combined sources3
Turni108 – 114Combined sources7
Beta strandi116 – 122Combined sources7
Helixi128 – 135Combined sources8
Helixi141 – 143Combined sources3
Beta strandi144 – 150Combined sources7
Helixi154 – 171Combined sources18
Helixi175 – 177Combined sources3
Beta strandi180 – 186Combined sources7
Helixi204 – 224Combined sources21
Beta strandi229 – 235Combined sources7
Beta strandi239 – 241Combined sources3
Helixi246 – 251Combined sources6
Beta strandi256 – 268Combined sources13
Turni269 – 272Combined sources4
Beta strandi273 – 278Combined sources6
Helixi288 – 295Combined sources8
Turni308 – 312Combined sources5
Helixi317 – 320Combined sources4
Beta strandi331 – 335Combined sources5
Beta strandi338 – 344Combined sources7
Helixi345 – 349Combined sources5
Helixi364 – 374Combined sources11
Beta strandi379 – 389Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZF8X-ray1.98A93-395[»]
ProteinModelPortaliP39107.
SMRiP39107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ANP1/MMN9/VAN1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP39107.
KOiK05529.
OMAiPYDFNNW.
OrthoDBiEOG092C2GA4.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH
60 70 80 90 100
KWAHEKENTF YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD
110 120 130 140 150
LNKLHSTSEA AVNKEHILIL TPMQTFHQQY WDNLLQLNYP RELIELGFIT
160 170 180 190 200
PRTATGDLAL KKLENAIKKV QTDKKTQRFS KITILRQNSQ SFDKLMEKER
210 220 230 240 250
HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII ETPPSLIQDM
260 270 280 290 300
TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD
310 320 330 340 350
EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD
360 370 380 390
GAMFPNFPFY HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH
Length:395
Mass (Da):45,955
Last modified:January 23, 2007 - v3
Checksum:i461783BB4D700987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23752 Genomic DNA. Translation: AAA53677.1.
U44030 Genomic DNA. Translation: AAB68171.1.
BK006949 Genomic DNA. Translation: DAA11380.1.
PIRiS43746.
RefSeqiNP_015275.1. NM_001183864.1.

Genome annotation databases

EnsemblFungiiYPL050C; YPL050C; YPL050C.
GeneIDi856057.
KEGGisce:YPL050C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23752 Genomic DNA. Translation: AAA53677.1.
U44030 Genomic DNA. Translation: AAB68171.1.
BK006949 Genomic DNA. Translation: DAA11380.1.
PIRiS43746.
RefSeqiNP_015275.1. NM_001183864.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZF8X-ray1.98A93-395[»]
ProteinModelPortaliP39107.
SMRiP39107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36130. 31 interactors.
DIPiDIP-783N.
IntActiP39107. 21 interactors.
MINTiMINT-552187.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

Proteomic databases

MaxQBiP39107.
PRIDEiP39107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL050C; YPL050C; YPL050C.
GeneIDi856057.
KEGGisce:YPL050C.

Organism-specific databases

EuPathDBiFungiDB:YPL050C.
SGDiS000005971. MNN9.

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP39107.
KOiK05529.
OMAiPYDFNNW.
OrthoDBiEOG092C2GA4.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-33963-MONOMER.

Miscellaneous databases

PROiP39107.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMNN9_YEAST
AccessioniPrimary (citable) accession number: P39107
Secondary accession number(s): D6W3W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.