P39107 (MNN9_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 106.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Mannan polymerase complexes subunit MNN9 Short name=M-pol I subunit MNN9 Alternative name(s): M-Pol II subunit MNN9 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 395 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex. Ref.7 |
| Pathway | |
| Subunit structure | The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.4 Ref.6 |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note: Cis-Golgi. Recycles between endoplasmic reticulum and Golgi. Ref.4 Ref.5 Ref.6 |
| Miscellaneous | Present with 1630 molecules/cell in log phase SD medium. Ref.8 |
| Sequence similarities | Belongs to the ANP1/MMN9/VAN1 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ANP1 | P32629 | 6 | EBI-11082,EBI-2595 | |
| VAN1 | P23642 | 5 | EBI-11082,EBI-20237 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.4 | ||||||
| Chain | 2 – 395 | 394 | Mannan polymerase complexes subunit MNN9 | PRO_0000193670 | |||||
Regions | |||||||||
| Topological domain | 2 – 17 | 16 | Cytoplasmic Potential | ||||||
| Transmembrane | 18 – 33 | 16 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 34 – 395 | 362 | Lumenal Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 236 | 1 | D → A: Reduced activity of the M-Pol I complex. Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins." Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L. Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed: 8146181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus." Hashimoto H., Yoda K. Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed: 9434768] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, SUBCELLULAR LOCATION, SUBUNIT. |
| [5] | "Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum." Todorow Z., Spang A., Carmack E., Yates J., Schekman R. Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed: 11095735] [Abstract] Cited for: PROTEIN SEQUENCE OF 143-153, SUBCELLULAR LOCATION. Strain: RSY455. |
| [6] | "Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity." Jungmann J., Munro S. EMBO J. 17:423-434(1998) [PubMed: 9430634] [Abstract] Cited for: ACTIVITY OF M-POL COMPLEXES, SUBUNIT, SUBCELLULAR LOCATION. |
| [7] | "The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis." Stolz J., Munro S. J. Biol. Chem. 277:44801-44808(2002) [PubMed: 12235155] [Abstract] Cited for: FUNCTION, COMPOSITION OF THE M-POL I COMPLEX, MUTAGENESIS OF ASP-236. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L23752 Genomic DNA. Translation: AAA53677.1. U44030 Genomic DNA. Translation: AAB68171.1. BK006949 Genomic DNA. Translation: DAA11380.1. |
| PIR | S43746. |
| RefSeq | NP_015275.1. NM_001183864.1. |
3D structure databases | |
| ProteinModelPortal | P39107. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-783N. |
| IntAct | P39107. 22 interactions. |
| MINT | MINT-552187. |
| STRING | P39107. |
Protein family/group databases | |
| CAZy | GT62. Glycosyltransferase Family 62. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YPL050C; YPL050C; YPL050C. |
| GeneID | 856057. |
| KEGG | sce:YPL050C. |
| NMPDR | fig|4932.3.peg.6408. |
Organism-specific databases | |
| SGD | S000005971. MNN9. |
Phylogenomic databases | |
| eggNOG | fuNOG05503. |
| GeneTree | EFGT00050000002721. |
| HOGENOM | HBG396793. |
| OMA | GLPNYLV. |
| OrthoDB | EOG4J9R7K. |
Gene expression databases | |
| ArrayExpress | P39107. |
| Genevestigator | P39107. |
| GermOnline | YPL050C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR005109. Anp1. [Graphical view] |
| KO | K05529. |
| Pfam | PF03452. Anp1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 981026. |
Entry information
| Entry name | MNN9_YEAST | ||||||||
| Accession | Primary (citable) accession number: P39107 Secondary accession number(s): D6W3W4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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