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P39107 (MNN9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan polymerase complexes subunit MNN9

Short name=M-pol I subunit MNN9
Alternative name(s):
M-Pol II subunit MNN9
Gene names
Name:MNN9
Ordered Locus Names:YPL050C
ORF Names:P7102.01
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex. Ref.7

Pathway

Protein modification; protein glycosylation.

Subunit structure

The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.4 Ref.6

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note: Cis-Golgi. Recycles between endoplasmic reticulum and Golgi. Ref.4 Ref.5 Ref.6

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ANP1/MMN9/VAN1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ANP1P326296EBI-11082,EBI-2595
VAN1P236423EBI-11082,EBI-20237

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 395394Mannan polymerase complexes subunit MNN9
PRO_0000193670

Regions

Topological domain2 – 1716Cytoplasmic Potential
Transmembrane18 – 3316Helical; Signal-anchor for type II membrane protein; Potential
Topological domain34 – 395362Lumenal Potential

Experimental info

Mutagenesis2361D → A: Reduced activity of the M-Pol I complex. Ref.7

Secondary structure

............................................. 395
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P39107 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 461783BB4D700987

FASTA39545,955
        10         20         30         40         50         60 
MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH KWAHEKENTF 

        70         80         90        100        110        120 
YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD LNKLHSTSEA AVNKEHILIL 

       130        140        150        160        170        180 
TPMQTFHQQY WDNLLQLNYP RELIELGFIT PRTATGDLAL KKLENAIKKV QTDKKTQRFS 

       190        200        210        220        230        240 
KITILRQNSQ SFDKLMEKER HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII 

       250        260        270        280        290        300 
ETPPSLIQDM TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD 

       310        320        330        340        350        360 
EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD GAMFPNFPFY 

       370        380        390 
HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins."
Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L.
Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26109 / X2180.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus."
Hashimoto H., Yoda K.
Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, SUBCELLULAR LOCATION, SUBUNIT.
[5]"Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum."
Todorow Z., Spang A., Carmack E., Yates J., Schekman R.
Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 143-153, SUBCELLULAR LOCATION.
Strain: RSY455.
[6]"Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity."
Jungmann J., Munro S.
EMBO J. 17:423-434(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVITY OF M-POL COMPLEXES, SUBUNIT, SUBCELLULAR LOCATION.
[7]"The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis."
Stolz J., Munro S.
J. Biol. Chem. 277:44801-44808(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COMPOSITION OF THE M-POL I COMPLEX, MUTAGENESIS OF ASP-236.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23752 Genomic DNA. Translation: AAA53677.1.
U44030 Genomic DNA. Translation: AAB68171.1.
BK006949 Genomic DNA. Translation: DAA11380.1.
PIRS43746.
RefSeqNP_015275.1. NM_001183864.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZF8X-ray1.98A93-395[»]
ProteinModelPortalP39107.
SMRP39107. Positions 95-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36130. 29 interactions.
DIPDIP-783N.
IntActP39107. 21 interactions.
MINTMINT-552187.
STRING4932.YPL050C.

Protein family/group databases

CAZyGT62. Glycosyltransferase Family 62.

Proteomic databases

MaxQBP39107.
PaxDbP39107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL050C; YPL050C; YPL050C.
GeneID856057.
KEGGsce:YPL050C.

Organism-specific databases

SGDS000005971. MNN9.

Phylogenomic databases

eggNOGNOG73762.
GeneTreeENSGT00390000005201.
HOGENOMHOG000167461.
KOK05529.
OMAPYDFNNW.
OrthoDBEOG73V6VP.

Enzyme and pathway databases

BioCycYEAST:G3O-33963-MONOMER.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP39107.

Family and domain databases

InterProIPR005109. Anp1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF03452. Anp1. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Other

NextBio981026.

Entry information

Entry nameMNN9_YEAST
AccessionPrimary (citable) accession number: P39107
Secondary accession number(s): D6W3W4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways