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Reviewed, UniProtKB/Swiss-Prot P39107 (MNN9_YEAST)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Mannan polymerase complexes subunit MNN9
      Short name=M-pol I subunit MNN9
Alternative name(s):
    M-Pol II subunit MNN9
Gene names
Name: MNN9
Ordered Locus Names: YPL050C
ORF Names: P7102.01
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex. Ref.6

Pathway

Protein modification; protein glycosylation.

Subunit structure

The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.3 Ref.5

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note: Cis-Golgi. Recycles between endoplasmic reticulum and Golgi. Ref.3 Ref.5 Ref.4

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the ANP1/MMN9/VAN1 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 395394Mannan polymerase complexes subunit MNN9
PRO_0000193670

Regions

Topological domain2 – 1716Cytoplasmic Potential
Transmembrane18 – 3316Signal-anchor for type II membrane protein Potential
Topological domain34 – 395362Lumenal Potential

Experimental info

Mutagenesis2361D → A: Reduced activity of the M-Pol I complex. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P39107-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 461783BB4D700987

FASTA39545,955
        10         20         30         40         50         60 
MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH KWAHEKENTF 

        70         80         90        100        110        120 
YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD LNKLHSTSEA AVNKEHILIL 

       130        140        150        160        170        180 
TPMQTFHQQY WDNLLQLNYP RELIELGFIT PRTATGDLAL KKLENAIKKV QTDKKTQRFS 

       190        200        210        220        230        240 
KITILRQNSQ SFDKLMEKER HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII 

       250        260        270        280        290        300 
ETPPSLIQDM TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD 

       310        320        330        340        350        360 
EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD GAMFPNFPFY 

       370        380        390 
HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins."
Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L.
Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed: 8146181] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26109 / X2180.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus."
Hashimoto H., Yoda K.
Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed: 9434768] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, SUBCELLULAR LOCATION, SUBUNIT.
[4]"Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum."
Todorow Z., Spang A., Carmack E., Yates J., Schekman R.
Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed: 11095735] [Abstract]
Cited for: PROTEIN SEQUENCE OF 143-153, SUBCELLULAR LOCATION.
Strain: RSY455.
[5]"Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity."
Jungmann J., Munro S.
EMBO J. 17:423-434(1998) [PubMed: 9430634] [Abstract]
Cited for: ACTIVITY OF M-POL COMPLEXES, SUBUNIT, SUBCELLULAR LOCATION.
[6]"The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis."
Stolz J., Munro S.
J. Biol. Chem. 277:44801-44808(2002) [PubMed: 12235155] [Abstract]
Cited for: FUNCTION, COMPOSITION OF THE M-POL I COMPLEX, MUTAGENESIS OF ASP-236.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

L23752 Genomic DNA. Translation: AAA53677.1.
U44030 Genomic DNA. Translation: AAB68171.1.
PIRS43746.
RefSeqNP_015275.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:783N.
IntActP39107. 24 interactions.

Protein family/group databases

CAZyGT62. Glycosyltransferase Family 62.

Genome annotation databases

EnsemblYPL050C. Saccharomyces cerevisiae. [Contig view]
GeneID856057.
GenomeReviewsGene locus YPL050C in contig U00094_GR.
KEGGsce:YPL050C.
NMPDRfig|4932.3.peg.6408.

Organism-specific databases

CYGDYPL050c.
SGDS000005971. MNN9.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP39107.
OMAP39107. GLPNYLV.

Gene expression databases

GermOnlineYPL050C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR005109. Anp1.
[Graphical view]
PfamPF03452. Anp1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio981026.

Entry information

Entry nameMNN9_YEAST
AccessionPrimary (citable) accession number: P39107
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents