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P39107

- MNN9_YEAST

UniProt

P39107 - MNN9_YEAST

Protein

Mannan polymerase complexes subunit MNN9

Gene

MNN9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The M-Pol I and M-Pol II complexes possess alpha-1,6-mannosyltransferase activity and are probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. May also provide alpha-1,2-mannosyltransferase activity to the M-Pol I complex.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell wall mannoprotein biosynthetic process Source: UniProtKB
    2. mannosylation Source: GOC
    3. protein N-linked glycosylation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33963-MONOMER.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT62. Glycosyltransferase Family 62.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan polymerase complexes subunit MNN9
    Short name:
    M-pol I subunit MNN9
    Alternative name(s):
    M-Pol II subunit MNN9
    Gene namesi
    Name:MNN9
    Ordered Locus Names:YPL050C
    ORF Names:P7102.01
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000005971. MNN9.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha-1,6-mannosyltransferase complex Source: SGD
    2. cis-Golgi network Source: SGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. Golgi cis cisterna Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi236 – 2361D → A: Reduced activity of the M-Pol I complex. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 395394Mannan polymerase complexes subunit MNN9PRO_0000193670Add
    BLAST

    Proteomic databases

    MaxQBiP39107.
    PaxDbiP39107.

    Expressioni

    Gene expression databases

    GenevestigatoriP39107.

    Interactioni

    Subunit structurei

    The M-Pol I complex contains MNN9 and VAN1. The M-Pol II complex is composed of ANP1, MNN9, MNN10, MNN11 and HOC1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ANP1P326296EBI-11082,EBI-2595
    VAN1P236423EBI-11082,EBI-20237

    Protein-protein interaction databases

    BioGridi36130. 29 interactions.
    DIPiDIP-783N.
    IntActiP39107. 21 interactions.
    MINTiMINT-552187.
    STRINGi4932.YPL050C.

    Structurei

    Secondary structure

    1
    395
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi101 – 1033
    Turni108 – 1147
    Beta strandi116 – 1227
    Helixi128 – 1358
    Helixi141 – 1433
    Beta strandi144 – 1507
    Helixi154 – 17118
    Helixi175 – 1773
    Beta strandi180 – 1867
    Helixi204 – 22421
    Beta strandi229 – 2357
    Beta strandi239 – 2413
    Helixi246 – 2516
    Beta strandi256 – 26813
    Turni269 – 2724
    Beta strandi273 – 2786
    Helixi288 – 2958
    Turni308 – 3125
    Helixi317 – 3204
    Beta strandi331 – 3355
    Beta strandi338 – 3447
    Helixi345 – 3495
    Helixi364 – 37411
    Beta strandi379 – 38911

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZF8X-ray1.98A93-395[»]
    ProteinModelPortaliP39107.
    SMRiP39107. Positions 95-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1716CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 395362LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 3316Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ANP1/MMN9/VAN1 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG73762.
    GeneTreeiENSGT00390000005201.
    HOGENOMiHOG000167461.
    KOiK05529.
    OMAiPYDFNNW.
    OrthoDBiEOG73V6VP.

    Family and domain databases

    InterProiIPR005109. Anp1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF03452. Anp1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39107-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH    50
    KWAHEKENTF YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD 100
    LNKLHSTSEA AVNKEHILIL TPMQTFHQQY WDNLLQLNYP RELIELGFIT 150
    PRTATGDLAL KKLENAIKKV QTDKKTQRFS KITILRQNSQ SFDKLMEKER 200
    HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII ETPPSLIQDM 250
    TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD 300
    EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD 350
    GAMFPNFPFY HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH 395
    Length:395
    Mass (Da):45,955
    Last modified:January 23, 2007 - v3
    Checksum:i461783BB4D700987
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23752 Genomic DNA. Translation: AAA53677.1.
    U44030 Genomic DNA. Translation: AAB68171.1.
    BK006949 Genomic DNA. Translation: DAA11380.1.
    PIRiS43746.
    RefSeqiNP_015275.1. NM_001183864.1.

    Genome annotation databases

    EnsemblFungiiYPL050C; YPL050C; YPL050C.
    GeneIDi856057.
    KEGGisce:YPL050C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23752 Genomic DNA. Translation: AAA53677.1 .
    U44030 Genomic DNA. Translation: AAB68171.1 .
    BK006949 Genomic DNA. Translation: DAA11380.1 .
    PIRi S43746.
    RefSeqi NP_015275.1. NM_001183864.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZF8 X-ray 1.98 A 93-395 [» ]
    ProteinModelPortali P39107.
    SMRi P39107. Positions 95-394.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36130. 29 interactions.
    DIPi DIP-783N.
    IntActi P39107. 21 interactions.
    MINTi MINT-552187.
    STRINGi 4932.YPL050C.

    Protein family/group databases

    CAZyi GT62. Glycosyltransferase Family 62.

    Proteomic databases

    MaxQBi P39107.
    PaxDbi P39107.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL050C ; YPL050C ; YPL050C .
    GeneIDi 856057.
    KEGGi sce:YPL050C.

    Organism-specific databases

    SGDi S000005971. MNN9.

    Phylogenomic databases

    eggNOGi NOG73762.
    GeneTreei ENSGT00390000005201.
    HOGENOMi HOG000167461.
    KOi K05529.
    OMAi PYDFNNW.
    OrthoDBi EOG73V6VP.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:G3O-33963-MONOMER.

    Miscellaneous databases

    NextBioi 981026.

    Gene expression databases

    Genevestigatori P39107.

    Family and domain databases

    InterProi IPR005109. Anp1.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF03452. Anp1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins."
      Yip C.L., Welch S.K., Klebl F., Gilbert T., Seidel P., Grant F.J., O'Hara P.J., Mackay V.L.
      Proc. Natl. Acad. Sci. U.S.A. 91:2723-2727(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26109 / X2180.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus."
      Hashimoto H., Yoda K.
      Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11, SUBCELLULAR LOCATION, SUBUNIT.
    5. "Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum."
      Todorow Z., Spang A., Carmack E., Yates J., Schekman R.
      Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 143-153, SUBCELLULAR LOCATION.
      Strain: RSY455.
    6. "Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity."
      Jungmann J., Munro S.
      EMBO J. 17:423-434(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVITY OF M-POL COMPLEXES, SUBUNIT, SUBCELLULAR LOCATION.
    7. "The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis."
      Stolz J., Munro S.
      J. Biol. Chem. 277:44801-44808(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COMPOSITION OF THE M-POL I COMPLEX, MUTAGENESIS OF ASP-236.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMNN9_YEAST
    AccessioniPrimary (citable) accession number: P39107
    Secondary accession number(s): D6W3W4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1630 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3